Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx

... 2004 Synergy in activation of the IFN-b gene (Eur. J. Biochem. 271) 3703 Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon- b ... arrange-ment of the cis-acting elements in the promoter and to the nature of the factors they bind. To investigate the effect of promoter context on the transcription al activity of IRFs, weused ... IFN-b gene and that there are interactions unique to the arrangement of regulatory elements in the promoter that favor the involv-ment of IRF-7 in its activation. Ó FEBS 2004 Synergy in activation...

Báo cáo khoa học: Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase docx

... (from 2 to 9 in increments of 0.5) in buffers containing 150 mm NaCl by incubating at37 °C for 3 h. Again, there was no evidence for the production of a speciﬁc product; instead, the reactionmixture ... varying amounts of BTH and for different times as indicated: (B) 0.05 lg of BTH, 5 min incuba-tion; (C) 0.5 lg of BTH, 5 min incubation; (D) 5 lg of BTH, 5 minincubation; (E) 5 lg of BTH, 10 min ... these conclusions, and in addition showed the impor-tance of the reducing end of the substrate for the enzyme to recognize the length of the saccharide.AbbreviationsBTH, bovine testicular hyaluronidase;...

Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt

... switch in renal cell carcinoma (RCC) is attributed to the inactivation of the von Hippel–Lindau tumor suppressor, stabilization of hypoxia inducible factor- 1 transcription factor and increased ... potent and versatile angiogenesis inhibitorthat is critical for the maintenance of the antiangiogen-ic microenvironment in multiple organ sites, includingbreast, brain, colon and skin [19]. ... one of the most studied angiogenesis inhibi-tors [21], both in terms of regulation and mechanism of action. It is lost in multiple tumor types: ﬁbrosar-coma, glioblastoma and carcinomas of the...

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the ... the b-strandsb2, b3, b4 and b8 of the (b ⁄ a)8barrel domain, and for rBAT also with the short stretch near the C-terminus of domain B [9,19]. From the sequence–structure point of view, the basic ... in region I covering the b3-strand; (b) a segment of up to ﬁve residues(307_GVDGF in human rBAT) preceding the func-tional arginine (arginine 197 in oligo-1,6-glucosidase) in region II of the...

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... changes in the cytoskeleton thatmay occur during the initial 6 h window of infection.We infected RPE cells with K26GFP [27], and stainedcells for F-actin, using phalloidin at 30 min and 6 hpostinfection, ... fullyunderstand HSV-1 infection of all target cells, includ-ing RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies ... nec-tins induce formation of ﬁlopodia and lamellipodiathrough the respective activation of Cdc42 and Racsmall G proteins. J Biol Chem 277, 50749–50755.Supporting information The following supplementary...

Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx

... set of control data was obtained in the absence of the protein. The data set obtained in the absence of the protein was then subtracted from the correspondingdata set obtained in the presence of ... (association and dissociation).Therefore, the rate constants for the binding of DHNP and DHMP were ﬁxed at the values measured for the corresponding oxidized pterins NP and MP during the initial ... experiments. We ran the forwardreaction (the formation of HP) using both DHNP and DHMP as the substrates and the reverse reaction (the formation of DHNP and DHMP) with HP and GA. For the forward reaction,...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... demonstrating that the individualbinding constants of divalent metal ions for the a domain are larger than those for the b domain.Further interpretation of these data resulted in the proposal of positively ... elucidate the meta-llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation details of the two-domain protein. The results presented ... ﬁve of the 11cysteinyl sulfurs act as bridging ligands between twometal centers, and the remaining six act as terminalligands by coordinating to a single metal center. The numbering of the...

Tài liệu Báo cáo khoa học: Progress for dengue virus diseases Towards the NS2B–NS3pro inhibition for a therapeutic-based approach ppt

... 40–66 of the cofactor is important in the folding of the protein and that the C-terminal part of the cofactor,which is absent in the truncated form, directly interactswith the substrate-binding ... interaction between the NS2B cofactor and NS3pro seems to be important not only for the correctfold of the protease but also for the correct interactionwith the substrate. The binding site of the NS2B ... of the substrate. The substrate is oriented by the binding of the amino acid side chain of the P1resi-due in the S1pocket [96], a hydrogen bond between the backbone NH of the P1residue and...

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

... activity in crude yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. The tagged protein was puriﬁed and then used for furtheranalysis. In the ... nkatÆmg)1. The GLD1 pro-tein was tagged with a histidine tag at the N-terminalend by adding the coding sequence for six histidines to the end of the open reading frame, and then expressed in S. ... The tagged protein had a similar activ-ity in the crude extract as the nontagged protein, indi-cating that the tag did not affect the protein activity. The tagged protein was then puriﬁed and...

Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx

... couldparticipate in proton translocation or, in the case of E103, in the binding of the twin-arginine motif in the signal peptide. The absence of any translocationdefects leads us to conclude that these ... 2275Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocaseClaire M. L. Barrett and Colin RobinsonDepartment of Biological ... synthesis with cleavable, N-ter-minal signal peptides that almost invariably contain anessential twin-arginine motif in the N-terminal domain[9,10]. They then interact with a translocon in the inner...

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