Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
... Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein Cristina Lanni, Michela Mazzucchelli, Emanuela ... overexpres-sion of the PKCe V1 region, which binds specifically to the receptor for activated C -kinase (RACK), blocking the activation of the kinase specifically [13]. These experimentsresulted in a reduced ... PKC in a cellular model of downregulation o f PKCe and/ or PKCa to understand the respective roles of these speci c isoforms of PKC in the activation of APP proteolytic processing.Materials and...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... Finland). The concentration of the oxidant in the samples was calculated using the standard curve,which was made by adding known concentrations of the authentic H2O2instead of the samples.Visualization ... examined the cytotoxic effects of selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the microtiter tetrazoliummethod [41–43]. The cytotoxicities of the compounds in ... mole-cule to the cell membrane upon stimulation withPMA. Figure 5 shows the red fluorescent phallodinstaining (F-actin) and the blue fluorescence of the nuclei. Green fluorescence marks the p47phoxtranslo-cated...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... mm Ca2+. According to calculationsusing the spherical shape of macromolecules, the hydrodynamic radius of the protein will increase by27% on formation of the dimer [22]. The increase in size ... activating the Ca2+release-activated Ca2+(CRAC) channel by first sensing the changes in Ca2+concentration in the endoplasmic reticulum ([Ca2+]ER) via itsluminal canonical EF-hand motif and ... EF-hand domainfrom STIM1, the helical content of the engineered protein CD2.STIM1.EF increased, indicating that the inserted EF-hand motif at least partially maintains the natural helical structure...
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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot
... synthesize the N-terminal fragment of the poneratoxin gene. Two others: forward 5¢-GCCGCCCGTGATACAGGCGATCCACGATGCGCAGAGGTAGTAATGAG-3¢ and reverse 5¢-AATTCTCATTACTACCTCTGCGCATCGTGGATCGCCTGTATCACGGG-3¢ ... poneratoxin gene [11]. Two oligonucleotides: forward5¢-2GATCCATGTTTCTTCCGCTTCTGATCCTTGGCTCTCTTCTGATGAC-3¢ and reverse 5¢-CGGCGTCATCAGAAGAGAGCCAAGGATCAGAAGCGGAAGAAACATG-3¢, were used to synthesize ... 12 nucleotidescomplementary to the 3¢ end of poneratoxin gene (number4): 5¢-TCTGCCTTTGCATGCTTTCTTCCGCTTCTG-3¢ and 5¢-GAATTCTCATTACTACCT-3¢. Finally, the mix-ture of these two PCR reactions...
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Báo cáo khoa học: Novel diadenosine polyphosphate analogs with oxymethylene bridges replacing oxygen in the polyphosphate chain pdf
... asexpected, none of the three enzymes can cleave the P–O bond in the P-O-CH2-P linkage. Second, the asymmetric cleaving enzymes accept the P-O -C- Pbridge in the position adjacent to the P-O-P cleavagelocus ... in the a,b active site. Takentogether, the results of cleavage of compounds 3 and 5 show that the asymmetrically acting Ap4A hydro-lases can reach the scissile bond either by extending the ... Saccharomyces cerevisiae.FEBS Lett 561, 83–88.12 Guranowski A (2000) Speci c and nonspeci c enzymes involved in the catabolism of mononucleoside and dinucleoside polyphosphates. Pharmacol Ther...
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Báo cáo khoa học: "A Dynamic Bayesian Framework to Model Context and Memory in Edit Distance Learning: An Application to Pronunciation Classification" ppt
... Z; and consistency nodes sc and tc, which enforce the con-sistency constraint discussed in section 2. Because of symmetry we will explain the upper half of the graph involving the source string ... Distance introduced in the papertakes local statistical dependencies into account, the edit costsare still fixed and not corpus-driven.4 The concept of d-separation is useful to read independencerelations ... symbol is indeed consumed.Note that we can obtain the equivalent of the to-tal edit distance cost by using Viterbi inference and adding a costivariable as a deterministic child of the random...
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Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx
... (2005) Relaxin-3 ⁄ insulin-like peptide 5 chimeric peptide, a selective ligand for G protein- coupled receptor (GPCR)135 and GPCR142over leucine-rich repeat-containing G protein- coupledreceptor ... expression in neurons of the nucleus incertus of the dorsal pons, and a few other regions of the brain-stem. On the other hand, relaxin-3-expressing nerve fibers and the relaxin-3receptors, RXFP3 and ... fluo-rescent protein gene, we observed that the increase in intracellular cAMP induced by dibutyryl cAMP and forskolin treatment increased relaxin-3 promoter activ-ity. These increases were inhibited...
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Báo cáo khoa học: Tryptophan 243 affects interprotein contacts, cofactor binding and stability in D-amino acid oxidase from Rhodotorula gracilis ppt
... DAAO.Kinetics of reconstitution of apoprotein with FADBecause of the weaker binding of the FAD cofactor to the apoprotein of the W243 mutants, we studied the kinetics of reconstitution of the apoprotein–FAD ... titrating the apoprotein with increasing amounts of FAD and following the decrease in protein fluorescence: a Kdvalue of 0.37 and 0.035 lm of the apoprotein–FADcomplex was obtained for the W243I ... to Glu322), con-necting b-strands F5 and F6 and the negatively chargedresidues belonging to the a-helices I3¢ and I3¢¢ of the other monomer and (b) the interactions in the core of the dimer (where...
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Báo cáo khoa học: Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebrafish (Danio rerio) docx
... NY.17 Wu Q, Andolfatto P & Haunerland NH (2001) Cloning and sequence of the gene encoding the muscle fatty acidbinding protein from the desert locust, Schistocercagregaria. Insect Biochem Mol ... shown in capitalletters, with the coding sequences of eachexon underlined and the deduced aminoacid sequence indicated below. The nucleo-tide positions in the gene sequence areindicated by the ... muta-tions accumulate in the protein coding region, leadingto gene silencing and subsequent loss from the gen-ome, the most common fate of a duplicated gene; and neofunctionalization, in which mutation...
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Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation potx
... aggregation and assisting in proper folding [2]. The other class of proteinsincludes enzymes that catalyze speci c steps of protein folding. This group of proteins includes disulfide isomerases,which catalyze ... discussionDetection of a protein with increased cellular contentat a low temperatureCellular contents of the proteins in the bacterial cells areoften affected by the culture condition of these cells. ... kDa and an isoelectric point of four greatly increased at 4 C compared to that at 20 C. Determination of the N-terminal amino acid sequence, fol-lowed by the cloning and sequencing of the...
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