Báo cáo khoa học: The transmembrane domain of subunitbof theEscherichia coli F1FOATP synthase is sufficient for H + -translocating activity together with subunitsaandc doc
... The transmembrane domain of subunit
b
of the
Escherichia coli
F
1
F
O
ATP synthase is sufficient for H
+
-translocating activity
together with subunits
a
and
c
Jo¨ rg-Christian Greie, Thomas Heitkamp ... photosynthesis. Although the distinct com-
position of this multisubunit enzyme complex varies some-
what between species, all F
1
F
O
complexes share h...
... mechanism, it was proposed
that the C-terminal 17 residues of the A domain of
FnBPB form the latching peptide and play a similar
role in the interaction of FnBPB with Fg. To test this
hypothesis, ... objective of this project was to determine whether
the A domain of FnBPB binds Fg by the same mecha-
nism as the A domain of ClfA. A three-dimensional
molecular...
... is the fact that topoisomer-
ase activity is associated with the methylase/endonuclease
with a 5¢-CG-3¢ in the recognition sequence. In the light of
the similarities at the amino-acid level observed ... (Figs 2A,B). The first
15 amino acids of fragment p26 are LKLIRSKLDLTQK
QA. The sequence obtained for the first 20 amino acids of
p18 is GIPQKRKRFYLVAFLNQNI...
... thus showing the existence of such dimers in the inner
mitochondrial membrane [16]. Whether oligomeric forms of
the ATP synthase exist in the inner mitochondrial mem-
brane is still a matter of ... analysis the bands were revealed by the ATPase activity. The
oligomeric form (lane 1), the high band (lane 2), the low band (lane 3)
of the dimeric forms and the...
... same
characteristics of the interior of the protein matrix. The
script a is the peak–peak distance between the maximum
at %287 nm and the minimum at %283 nm, and the
script b is the peak–peak distance ... address the analysis of the
complex with information about the structure of the
individual complexes. Unfortunately little is known about
the 3D str...
... access
for the nuclease compared with the distal portion of
this SL which contains more G–C base pairs. Con-
trary to the left arm of the predicted SL1 structure,
few bases within the right arm showed ... determined
that the ROQ domain in the N-terminus of the protein
is the minimal region required for stress granule induc-
tion and localization. It is stri...
... not
required with respect to the role of HSP70 in the inhi-
bition of the release of Smac and apoptosis.
The role of the ATP-binding domain of HSP70
in the prevention of the release of Smac and
apoptosis ... role of HSP70, which comprises a
mechanism that involves the inhibition of Smac release
from mitochondria, and the suppression of caspase
activat...
... radiologist on
the day the CXR was performed. Similar to the ICU physicians,
the radiologist structurally interpreted the CXR for each
patient (for example, the radiologist ticked whether radiologi-
cal ... read the medical records, checked
the patient data management system (Metavision, iMDsoft,
Sassenheim, The Netherlands) and searched the hospital
information system...
... analyses is that the
exact binding location is not identified in the experi-
ments. Therefore, it is not clear whether the CDE ⁄ CHR
is involved in binding these factors.
Consistent with a function of ... transcription together with the
E2F site [20,21]. The DRS ⁄ CHR in the B-Myb pro-
moter deviates most from other CHR sequences with
its two-nucleotide exchange f...
... result of the
different pHs used in the NMR experiments [54]. The
structure of the monomer with the transiently formed
helix was determined at neutral pH, whereas that of
the monomer with the structured ... model of the NTD hexamer from MLV,
the superimposed model of the NTD hexamer from
HIV-1, the high-resolution X-ray model of the modified
CA hexamer f...