Báo cáo khoa học: Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles pdf
Báo cáo khoa học: Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles pdf
... investi-gated in submitochondrial particles by studying the IF1- mediated ATPase inhibition in the presence and absence of a protonmotive force. In the presence of protonmotiveforce, IF1 added during ... Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles Mikhail Galkin1,*, Rene´e Venard1, ... shown in Fig. 3B. Curve 3 (dashed) was obtained by mul-tiplying ordinates of curve 1 by the ratio between uncoupled and coupled GTPase rates in the absence of IF1. It indicates expected uncoupled...
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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf
... immunoblotting data, it was of interestto re-examine the fate of cals equestrin by studying the entirecomplement of key Ca2+-binding elements in dystrophin-deficient skeletal muscle fibres. Because the ... n the expression of Ca2+-bindingproteins. The relatively unique combination o f the pI valueand molecular mass of individual 2D spots can be useful in the initial identification of proteins. ... USA)withIMAGEQUANTV3.0 software. Major Ca2+-bindingproteins were identified by labelling with the cationiccarbocyanine dye, ÔStains-AllÕ, according t o the metho d of Campbell et al. [32]. Following the second...
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Báo cáo khoa học: Dual modulation of prothrombin activation by the cyclopentapeptide plactin pptx
... (A) The bind-ing of [14C]plactin-50 to human prothrombin was determined in the presence of the indicated concentrations of [14C]plactin-50. Specificbinding data are shown. (B) The intrinsic ... 4). These properties of the plactin–prothrombin interaction suggested a change in the conformation of prothrombin after plactin bind-ing. We measured the intrinsic fluorescence of prothrombin ... (second incubation). After incubation, cellularuPA activity was determined using a chromogenic u-PA substrate in the presence of aprotinin, an inhibitor of plasmin. Line indicates the average of...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
... by xanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto-chrome c being reduced by superoxide in the course of the reaction. The principle of ... catalysis, ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], ... used to examine the coordination of Fe2+ in the active site of the resting enzyme. The best fit of the extended X-ray absorption fine structure (EX-AFS) data indicated a five-coordinate or six-coordi-nate...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... in the size of the side chain may create steric hindrancefor the substrate, making the base moiety of the sub-strates bind in a nonoptimal conformation.Another interesting feature concerning ... acids lining thisspace. Binding of BVDU to Dm-dNK-Y70W compen-sates for the loss of one hydrogen bond to 3¢-OH by atighter fitting of the bromovinyl group, thus restoring the tight binding lost ... ion and arginines of the Lid-region sta-bilize the negative phosphates of this state. The moststriking effect of mutation of arginine to histidine is analmost 2000-fold decrease in the kcatvalue...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identification of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 ... eletion of 111 amino acids f rom the N-terminal region (BMAL1:112–626) did not affect the binding of BMAL1 to DEC1; deletion of 235 amino acids(BMAL1:236–626) slightly diminished the binding,although ... with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for Ala...
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Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
... Llinas M (1988) Lysi-ne ⁄ fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96.20 Thorsen S (1975) Differences in the binding to fibrin of native ... contributeto this inhibitory effect.We then investigated the consequence of the inhibi-tion of plasminogen binding on plasmin formation by fibrin-bound tissue plasminogen activator (t-PA) or by CHO-K1 ... mechanism was further explored by measuring the effect of the mAbs on the interaction of purifiedplasmin and Lys-Pg with fibrin and cells. The binding of Lys-Pg was partially inhibited by both 34D3...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure ... base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junctioncomplex. In this study the ... lacking leucine in the presence of thiamine for inhibition of the nmt promoter. Forvisualization of Ydc2–GFP constructs, yeast cultures weregrown in selective media in the absence of thiamine...
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Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx
... rates in the presence of kirromycin The effect of kirromycin on the growth of GRd.tsf wasinvestigated by measuring growth rates of cultures in LBcontaining various concentrations of kirromycin. ... reaction in EF-Tu was investigated by studying the functional effects of deleting the motif in EF-Ts, both in vivo and in vitro. The motif was deleted in endogenous EF-Ts in E. coli strain UY211 by ... show the positions of the protruding coiled-coil motif (residues 187–226) and the C-terminal module. The terminal positions of the deletion of the coiled-coil motif and the insertion of the linker...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... containing the last 326 amino acids in the case of Pta-F1, the last 352amino acids in the case of Pta-F2, and the last 470 aminoacids in the case of Pta-F3 (Fig. 2).PCR reactions performed in ... also the function of the DRTGG domain. Taking into account the importantrole of Ptas, the results obtained in the present work,dissecting the different domains forming E. coli Pta,will help in ... assembly of this protein.On the other hand, in order to evaluate the contri-bution of the N-terminal end to the formation of the final oligomeric state of Pta, the native conformationalstate of the...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDENghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)Chiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP