Báo cáo khoa học: Elements of the C-terminal t peptide of acetylcholinesterase that determine amphiphilicity, homomeric and heteromeric associations, secretion and degradation docx
Báo cáo khoa học: Elements of the C-terminal t peptide of acetylcholinesterase that determine amphiphilicity, homomeric and heteromeric associations, secretion and degradation docx
... All of these results show that various elements and features of the t peptide contribute to determine the cellular fate of the protein and deserve furtherinvestigations. It is highly probable that ... less extended C-terminal segments of the t peptide, in Torpedo AChE T subunits, to determine the structural basis of the character-istic properties that the t peptide confers to AChE T subunits.Materials ... Oligomerization and secretion of the AChE t peptide (Eur. J. Biochem. 271) 1487 Elements of the C-terminal t peptide of acetylcholinesterase that determine amphiphilicity, homomeric and heteromeric associations, secretion...
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Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... yeastextract as the untagged protein, indicating that the tagwas not interfering with the protein activity. The tagged protein was purified and then used for furtheranalysis.In the reductive ... used: 5¢-gaattcagaatggcctccaagacgta-3¢ and 5¢-gaattcttattcctcctctggccaaa-3¢. The PCR product was cloned, similar to gld1, first in a TOPOvector and then in the expression vector p2159. The S. cere-visiae ... that the tag did not affect the protein activity. The tagged protein was then purified and further ana-lysed. The purified GLD1 showed activity with dl-glycer-aldehyde and NADPH as a cofactor. It...
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Báo cáo khoa học: Val216 decides the substrate specificity of a-glucosidase in Saccharomyces cerevisiae doc
... 5¢-AGAAGCCATTGCTGAGCAATTTTTGTTC-3¢(underlining i ndicates t he Bpu1102I restriction site) and t hereverse primer 5¢-AAAAAGCTTGCACTAATTTTATTTGAC-3¢ (underliningindicates the HindIII restriction site and stop ... 5¢-ATGACTATTTCTTCTGCACATCCAGAGACAGAAC-3¢ con tains the initiation codon,while the reverse primer 5¢-CTTTCTGCAGACTCATTCGCTGATATATATTC-3¢ linked a PstI restriction siteto the termination codon. PCR ... acidfragment of pYIM in pYMA were t he same as those of Mun/Bst. Therefore, these results indicate that the fragmentincluding consensus region II c ontributes to the determin-ation of the substrate...
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Báo cáo khoa học: "Completing on the partial basis parses of ill-formed sentences of discourse information" docx
... date collocation patterns: "use -(on)- desktop" and '%lder -(on)- desktop." such as the 1st sentence to the 20th, the 51st to the 70th, and the 701st to the 720th. Thus, ... analyses of other sentences in the same text, thus, attempting to generate the analy- sis most appropriate to the discourse. The results of our experiments show the effectiveness of this method; ... to determine whether it can be joined to the third parse, then the examination moves to the next parse, and so on. Two partial parses are joined if the root (head node) of either parse tree...
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Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro pot
... domain mutants. Letters above indicate the mutation: the first letter is the originalresidue, the number its position in the protein, and the second letter the residue that it was substituted into. ... residue of strand b4¢. The V260E mutation couldprevent the formation of this strand as glutamate acts as astrand breaker [61]. Altogether, these data suggest a strongstructural role for the terminal ... repeated at least twice and the independentexperiments gave similar results (integration efficienciesrelative to that of wild-type IN). The K225H mutant had anactivity close to that of the wild-type...
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Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"
... unaware that the study was being conducted.Ethical approval was not sought, because at the time auditswere not within the remit of the local ethics committee. Prior tointroduction of CPOE, ... al.R519prescribed. These intercepted errors were not administered to the patient because either the pharmacist intercepted the prescription before administration or the nurse recognised the error. A potentially ... 0.01; Table 3). It is of note that the only major errors encountered were the threemajor intercepted errors attributed to CPOE. It appears that CPOE was associated with more minor errors that did...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... (protists). On the other hand, it is not possible to exclude that in the future some new sequences of hcHAT-like proteins of nonmetazoan origin may become available that together with the results ... rooting the tree that is on the branch leading to the enzymes originating fromnon-Metazoa (the eventual outgroup).It is worth mentioning, however, that if the evolu-tionary tree of all proteins ... that possesses the amino acid transportation activity,although without interacting with the heavy subunit itis unable to reach the plasma membrane. Thus, the role of the heavy subunit is to recognize the...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... or tripalmitin asstandards. The experimental data were normalized to the intensity of the incident beam and corrected for the detec-tor response, the buffer scattering was substracted, and the statistical ... constants, determinedat about 10-fold lower protein concentration than the measurements of inactivation, show the sametrends with respect to the effects of NaCl, CaCl2 and EDTA as the inactivation ... contrast to the activation effect of calcium ions, inactivation at high calcium ion concen-trations is dependent on the counterion. In contrastto the stabilizing effects of salts according to the Hofmeister...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... identified the multiple transcription initi-ation sites of the HO-2 gene and confirmed that the HO-2 gene promoter is juxtaposed to the HSCARGgene in the opposite direction. Thus, the HO-2 gene and the ... Priorto the functional analysis of the HO-2 gene promoter,we determined its transcription initiation site by5¢-RACE, and this indicated that transcription isinitiated from multiple sites (Fig. ... with respect to the intensity for the 28S RNA signal. The intensity representing HO-1 or HO-2 mRNA at the 0 time (0 h) is considered to be 100%. The ratio of each normalized value to the 0 time...
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Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... (WT)bacteriophage and ts mutants. Two mutations that appearto lead to the formation of cavities, one in the hydrophobiccore (M88V) and the other in the protein–RNA interaction (T4 5S), decrease the ... assembling the whole particle. It is also interest-ing that the mutants had a ts phenotype, which means ahigh sensitivity to both high and low temperatures. The presence of cavities that confer the ... in the emission of the probe occurred (Fig. 7A), suggesting that the conformation of the pressure-denatured state isdifferent from that of the urea-denatured state.DiscussionIn the last 20...
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