Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx

Báo cáo khoa học: Amyloid structure – one but not the same: the many levels of ﬁbrillar polymorphism potx

... 2010 FEBSmolecular packing of the prion domain into the amy-loid structure [32]. However, the discovery of self-propagating variants of insulin [33], b2-microglobulin[34], Ab [35] and glucagon ... wherecombinations of spectroscopic, thermostability and seed extensionkinetic data indicate distinct protofilament structures. Scalebar = 50 nm. The emission intensity of ThT staining of type ... Tmappvaluesrepresent thermal melting midpoints during 90 °CÆh)1temperatureramping of 25 mgÆL)1fibrils in 25 mM glycine ⁄ HCl (pH 2.67). The CD spectrum shows the reproducible unique fingerprint features of fibrils...

Tài liệu Báo cáo khoa học: Domain deletions and substitutions in the modular protein evolution doc

... domain deletions are more com-mon at either of the protein termini, and that theiroccurrence is slightly higher at one of the termini,depending on the number of domains in the proteinand the ... C−terminusLength of the N terminus in % of the deleted domainLength of the C terminus in % of the deleted domainPfam, N−terminus Pfam, C −terminusNumber of occurencesNumber of occurences2500035000550000 ... size of the deleted domain was calculated for the proteins with the complete arrangement. To take into account the variability of the length of the complete domain, the length of the N-terminal...

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitriﬁcans pptx

... substrate carbinolamine intermediate;III, iminoquinone intermediate; IV, product Schiff base intermediate;V, aminoquinol intermediate. In the oxidative half-reaction the ami-noquinol intermediate ... substrates. The lack of inhibition observed with benzylamine (Fig. 5A) in comparison to the inhibition observed with tryptamine(Fig. 5B) suggests differences in binding of the twosubstrates within the ... kinetically in uentialionizations were identiﬁed in plots of klim⁄ Kd of pKavalues 7.1 and 9.3,again with the maximum value realized in the alkaline region. The poten-tial origin of these kinetically...

Tài liệu Báo cáo khoa học: Human intrinsic factor expressed in the plant Arabidopsis thaliana doc

... at the internal site …PSNPﬂGPGP. The key properties of the preparation obtained were identical to those of native IF: the binding curves of vitamin B12to recombinant IF and gastricIF were the ... this Cblbinder hampers the uptake of the minute amounts of the vitamin present in food. Only around 1% of the ingestedCbl can be absorbed by passive diffusion [6].Classical vitamin B12deﬁciency ... (IF) is the gastric protein that promotes the intestinal uptake of vitamin B12. Gastric IF from animalsources is used in diagnostic tests and in vitamin pills.However, administration of animal...

Tài liệu Báo cáo khoa học: "THERE STILL IS GOLD IN THE DATABASE MINE" potx

... and mine it very deeply, not necessarily discovering every aspect of the domain but requiring that the various aspects be integrated with one another to produce a coherent whole. Even in the ... of our art? Instead of assuming that the problem is one of using typed English to access and/or update a file or files in a single database system, let us define a spectrum of potential natural ... ambiguity (the word "yield" is ambiguous if there is both financial and productivity data in the knowledge base), and other complex linguistic phenomena. Let us go on to define the other...

Báo cáo khoa học: Adipocyte hyperplasia and RMI1 in the treatment of obesity doc

... molecules,including cyclins, cyclin-dependent kinases, their inhib-itors and the retinoblastoma protein (RB), as well asE2Fs.E2F and RB family members appear to participate in the regulation of cell-cycle ... December2010)doi:10.1111/j.1742-4658.2010.07980.x The escalating prevalence of obesity is one of the most pressing healthconcerns of the modern era, yet existing medicines to combat this globalpandemic are disappointingly limited in terms ... cyclin ⁄ cyclin-dependentkinase holoenzymes, releasing the E2F complex andthereby resulting in activation of the E2F target genes[9]. Fajas et al. [10] demonstrated that the E2F proteinfamily...

Báo cáo khoa học: Nanoparticles can induce changes in the intracellular metabolism of lipids without compromising cellular viability docx

... HIF-1ais involved directly in the accumulation of lipids trig-gered by QDs under the conditions of uncompromisedcell survival in the presence of serum.On the basis of the results obtained in this ... storage in LDs. The PI3K ⁄ Akt signaling pathwaystimulates lipogenesis via SREBP-1. FAS is the enzyme responsiblefor the synthesis of palmitate, the precursor of FFAs. The inhibition of signaling ... possibility; however, the inhibition of PI3K with LY294002 and the inhibition of FASwith cerulenin caused the disappearance of dropletsand prevented the formation of new droplets duringexposure to...

Báo cáo khoa học: C ﬁ G base mutations in the CArG box of c-fos serum response element alter its bending ﬂexibility Consequences for core-SRF recognition potx

... provide information on the localrepartition of the angles involved in the SREfoshelixbending.Relative effect of bending strainThere are several indications of a redistribution of the strains ... Indeed, the change in the array of the hydrogen bonds at thymine of SREGGfosis probably a sign of perturbation in the hydration scheme along the minor groove of the (A ⁄ T)domain. The G–C ... cm)1at 25 °C of about the sameintegral intensity, indicating a larger population of lin-ear conformers. The upshift and increase in intensity of the peak at 838 cm)1suggest that the backboneconformation...

Báo cáo khoa học: "PARSING VS. TEXT PROCESSING IN THE ANALYSIS OF DICTIONARY DEFINITIONS" pot

... passives inverted, inflected forms analyzed, and other modifications of the kind often brought under the rubric of "transformations." The LSP can do this sort of thing very welL The defining ... the node to the right of the taxonomy marker 'W") serving as a pointer to its own defining node. The resulting network is quite dense. We simplify by leaving out other parts of the ... most of the duplicate relational triples. Finding relational triples by text processing As the performance statistics above show, parsing is painfully slow. For the simple business of finding...

Báo cáo khoa học: Localizing matrix metalloproteinase activities in the pericellular environment doc

... andMMP-1 bind the a2b1 integrin via the I domain of the a2 integrin subunit and that the linker peptide and the hemopexin domain of MMP-1 are required for optimalbinding [39]. Basal keratinocytes ... in their function. The N-terminal pro-peptide acts to maintain the latency of the MMPs by the presence of a cysteine residue in the active sitecoordinated to the catalytic zinc ion [6–9]. The ... (known as the ‘cysteine switch’)involves proteolytic cleavages of the propeptide caus-ing a destabilization of the cysteine–zinc interaction[10]. The extra catalytic domains of the MMPs cancontribute...

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