... al.
2800 FEBS Journal 273 (2006) 2789–2800 ª 2006 The Authors Journal compilation ª 2006 FEBS
RNA reprogramming of a-mannosidase mRNA sequences
in vitro by myxomycete group IC1 and IE ribozymes
Tonje ... April
2006)
doi:10.1111/j.1742-4658.2006.05295.x
Trans-splicing group I ribozymes have been introduced in order to mediate
RNA reprogramming (including...
... DNA-binding motif in nucleolin [21–
27]. The RGG domain of FMRP, which is an RNA-
binding protein involved in nerve cell differentiation,
interacts with the G-quartet forming RNA [22–25]. In
addition, ... guanidini-
um group of the Arg in the RGG domain in the
C-terminal of EWS.
Results and Discussion
Several DNA-binding proteins that bind to G-quadru-
plex DNA have be...
... examined cal-
pain 7–IST1 interactions by in vitro and in vivo binding
experiments, using purified recombinant proteins and
cultured mammalian cells expressing epitope-tagged
proteins. We also investigated ... autolysis of mGFP–cal-
pain 7–Strep by GST–IST1 and GST–MIM in vitro.
Fig. S2. Intermolecular proteolysis of mGFP–cal-
pain 7
C290S
–Strep and mGFP–calpain 7 DM...
... protein concentra-
tion was determined using the Coomassie Plus Protein Assay
Reagent (Pierce) with bovine serum albumin as the standard.
Expression and purification of recombinant protein was
confirmed ... that of
the enzyme-containing solution and was expressed as nmols
of 2,6-dichloroindophenol reduced per mg of protein per
min. The extinction coefficient of 2,6-dichloroindophen...
... screening and identifying ligand binding to
protein receptors. Angewandte Chemie-International Edi-
tion 42, 864–890.
16 Mayer M & Meyer B (1999) Characterization of ligand
binding by saturation ... was minimized in two
stages. In the first stage, we kept the protein fixed and only
minimized the position of the water molecules and ions. In
A. Viegas et al. Determinants of...
... in ammatory cytokines, including IL-1b, TNF-a and
IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into
the joints. In addition to functioning in cell trafficking, several
chemokines have ... IFN-c, interferon-c; IL, interleukin; IP-10, interferon-c-inducible protein-10; MAPK,
mitogen-activated protein kinase; MCP, monocyte chemoattractant protein; Mig, monokine induced...
... that the N-terminal domain is involved in
protein–protein interactions with other hydrophilic domains
of neighboring integral membrane subunits, and interspecies
interactions are incompatible.
From ... [15–18]. In the
bovine complex a large subdomain is made up of 20
integral membrane proteins contributing > 60 transmem-
brane segments. Some of these must be intimately involv...
... clustering task, with over 15 research groups
submitting results in each campaign. Since the re-
lease of the first datasets, this task is becoming an
increasingly popular research topic among Infor-
mation ... types of features from each
document. First, we applied a simple preprocess-
ing to the HTML documents in the corpus, con-
verting them to plain text and tokenizing. Then,
we...
... (Table 1). The binding affinities of the inhibitor
to both binding sites, yielding the IE or EI complex, were
only slightly different. On the other hand, the binding
affinity of the second inhibitor ... Ala743 of the enzyme, between
the side chain carbonyl group of Asn7 of Tat(1–9) and the
side chain of Lys554 of DP IV and finally, between the
carbonyl group of Ile8 of...
... this
protease at pH9 [10]. Further interest in the aromatic
stacking is in the role of the electrostatic properties in
enzymatic catalysis of API, and in distinguishing the
functionally catalytic ... confirmed by energy minimization (Fig. 3). The charge
interaction between Asp113 and His210 is weakened with
increasing solvent-accessibility of the His210 side-chain. In
the...