... PCRtemplate. For PCR, the tag-specific primer was used as the sense primer, and consisted of the 14-base tagnucleotides and 5¢ inosine nucleotides to increase the annealing temperature of the primers ... sequence of the PCR product has been obtained, check the tag to see whether its location is right after the last CATG site before the polyA signal in the PCR product, if possible, before further analysis. ... excise and purify individual bands, and cloned into T-vectors.Step 6. Verification of tag sequenceOnce the complete sequence of the PCR product has been obtained, check the tag to see whether...