... Structure-activity of Drosophila ACEs
FEBS Journal 273 (2006) 362–373 ª 2005 The Authors Journal compilation ª 2005 FEBS 373
Structural diversity of angiotensin-converting enzyme
Insights from structure–activity ... (1995) Race: a
Drosophila homolog of the angiotensin-converting
enzyme. Mech Dev 51, 157–168.
22 Taylor CAM, Coates D & Shirras AD (1996) The Acer
g...
... similar levels of Neu5Ac [26] as well as glycine [20].
From the results (data not shown) of sugar (of LPS) and
methylation (of LPS-OH) analysis, ESI-MS (of LPS-OH
and OS),
1
H NMR (of LPS-OH) and ... ESI-MS) of the Hep3-glycoforms men-
tioned above.
Characterization of OS fractions from
lpsA
mutant
of NTHi isolate 1233 by NMR
NMR data indicated the major Hep3-glycoform (Hex1...
... representation of one coil of sbwAFP
(red, residues G ly34 to T hr49) and TmAFP (blue, residues
Asn29fiGly41). Letters de note the five residues of on e of the three
sides of s bwAFP o r s ix residues of on ... [53].
Role of the TXT motif and water in activity
Examination of the crystal structures of the insect AFPs
also revealed the presence of an array of water molecules...
... visualization of most of the
macromolecular compounds of the ASM on the same
gel. N63 exhibits an important shift of approximately
10 kDa, which represents a loss of apparent molecular
weight of 14%. ... formation of the shell nacreous
layer, we have investigated the biochemistry of Nautilin-63, one of the
main nacre matrix proteins of the cephalopod Nautilus macromphalus....
... substantially
lower that that of t he U4-hairpin. This suggests that poor
excision of U from the U2-hairpin could be a consequence
of its lower affinity to for the enzyme. A caveat to this
interpretation, ... major
groove side of the DNA to make a ppropriate contacts in the
active site of the enzyme. Presumably, the potential energy
required for these structural changes to occu...
... representative
ofthediversitywithinthetypefcluster.
Previous studies of LPS from H. influenzae have
resulted in a structural model consisting of a conserved
Fig. 4. Selected region of the two- dimensional
NOESY ... of LPS with Neu5Ac is widespread
among H. influenzae strains [7]. However, levels of
Fig. 5. Selected region of the two- dimensional
NOESY spectrum (mixing time 250...
... mainly built
up by residues from the C-terminus and consists of a
two- stranded anti-parallel b-sheet composed of b2 and
b9 and an a-helix from the C-terminus (Fig. 1A). The
two subunits in the asymmetric ... structural characterization of numerous com-
plexes of the human HPRT and several bacterial and
protozoan HPRTs have been undertaken [13–17]. The
structure of human HP...
... wide
range of spectroscopic techniques.
Suitable methods for investigation of the secondary
structure and morphology of such structures, however,
require the presence of large amounts of the oligomeric
state, ... distribu-
tion of 100 l
M proinsulin C-peptide in the
presence of divalent Ca
2+
and Mg
2+
ions
under native conditions (B), and of 100 l
M
C-peptide in the presence...
... is regulated in dual-
flavin enzymes like NOS is a topic of current interest.
Characteristics of NOS
NOS enzymes catalyze the NADPH- and O
2
-depen-
dent conversion of l-arginine (Arg) to citrulline ... studies
[17–22].
NOS enzymes have novel features
NOS are heme-thiolate enzymes and catalyze oxygen
activation by a mechanism similar to that of the cyto-
chrome P450 (CYP) enzy...
... respec-
tively [18]. All of the enzymes used in these syntheses
(excluding MR) are available from Sigma-Aldrich (St.
Louis, MO, USA) and the coenzymes are available
from Melford Laboratories ... transi-
tion state theory, the KIE arising from a full tunnelling
reaction (described by Eqns 1–3) can be any value
[10,46].
Preparation of coenzymes
The methods of coenzyme deuteration...