Báo cáo khoa học: Nerve inﬂuence on myosin light chain phosphorylation in slow and fast skeletal muscles pdf

... Bozzo et al. Myosin phosphorylation and denervationFEBS Journal 272 (2005) 5771–5785 ª 2005 FEBS 5785 Nerve in uence on myosin light chain phosphorylation in slow and fast skeletal muscles Cyril ... involved in the control of post-translational modiﬁcations of myosin, we analysed the phosphorylation of alkali myosin light chains (MLC1) and regulatory myo-sin light chains (MLC2) in rat slow ... (MM ⁄ BM), and distribution of slow and fast myosin heavy chain (MHC) and myosin light chain (MLC) isoforms determined by SDS ⁄ PAGE and densitometry in the soleus and EDL muscles of controluntreated...

Báo cáo khoa học: "A Comparative Study on Generalization of Semantic Roles in FrameNet" ppt

... verbs and nominalizations) into uniﬁed seman-tic representations, SRL has attracted much at-tention from researchers into various NLP appli-cations including question answering (Narayanan and ... group Giv-ing::Donor (in the hierarchical-relation groups)contains the roles Giving::Donor and Com-merce pay::Buyer. The remainder of this sectiondescribes the grouping criteria in detail.4.1 ... determinedautomatically in the training process.6 Experiment and DiscussionWe used the training set of the Semeval-2007Shared task (Baker et al., 2007) in order to ascer-tain the contributions...

Báo cáo khoa học: Action of palytoxin on apical H+/K+-ATPase in rat colon pdf

... ouabain (g-strophan-thine), the most well known inhibitor of the pump. Based on these and other experiments showing the formation ofpalytoxin-induced ion channels in membranes containing in vitro-translated ... latter findings, as the palytoxin-inducedconductance was insensitive to 1 mMouabain while beinginhibited at low concentrations of orthovanadate.Similar findings showing a palytoxin-induced K+effluxthat ... Thus, in the current investigation, wedescribe the action of palytoxin on the H+/K+-ATPasefrom the rat colon and demonstrate that the interaction ofthis toxin with the enzyme results in specific...

Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf

... mixture lacking heliquinomycin, and the proportions in the mixtures containing heliquinomycin were cal-culated in relation to the control value. The horizontal line is dis-played on a logarithmic ... RNA in the control reaction mixture lack-ing heliquinomycin was considered to be 100%, and that recordedfor the reaction mixtures containing heliquinomycin was presented in relation to this control ... of the control reaction mixture lacking heliquinomycin wasconsidered to be 100%, and that recorded for the reaction mixturecontaining heliquinomycin was presented in relation to this controlvalue....

Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

... possibilities for the binding of ligands on the ironof Fe(porphyrin))13G1 0 complexes: (A) binding of H2O2 on the iron of13G10-(Fe(ToCPP), (B) bind ing of two CN±ligands on the ironof 13G10-(Fe(ToCPP), ... edge -on bindingof Fe(ToCPP) occurs inside the binding pocket of 13G10(Fig. 7) that allows 2 CN±ligands to bind on the iron atom,one on each face of the porphyrin. This hyp othesis is in agreement ... imidazoleligands on the iron atom, and the only one that enters theactive site bind on the less hindered face of the porphyrinwhich bears only one ortho-COOH substituent (Fig. 7C).The inhibition of...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... identiﬁcation of complexes, binding to the VEGFmRNA 5¢ -and3 ¢-UTR, that contain cold shock domain(CSD) and polypyrimidine tract binding (PTB) RNAbinding proteins. Analysis of the CSD/PTB binding sitesrevealed ... [62]. Interestingly, we observed that theinsulin mRNA PTB binding site has an overlapping consen-sus CSD binding site. PTB, to date, has only been implicated in stability of insulin and VEGF ... 5¢-UTR contains sequences, at +1 to +22, that arerequired for mRNA stabilization in T cells. Both dbpB/YB-1 and another RNA binding protein, nucleolin, bind to thisregion and are involved in stabilization...

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... positive control [15].Immunoﬂuorescent staining for PLDNonopsonized zymosan was sonicated and added toneutrophils (25 · 106cellsÆmL)1 in NaCl/PicontainingCaCl2 and MgCl2) in a 5 : ... expression ofPLD2 (Fig. 5A). Identical data were obtained using eosi-nophil-depleted neutrophils, conﬁrming that these signalswere not consequent on the 1–5% eosinophil contaminationpresent in ... aspositive controls.The subcellular distribution of PLD and the conse-quences of priming and activation were determined usingimmunoprecipitation and Western blot analysis of neutro-phil cytosol and...

Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... oxidizing conditions. (c) Apo-ACP (O) and holo-ACP (h) in each fraction was quantiﬁed by measuring the intensity of the silver-stained protein bands usingQUANTITY ONE software and plotted against ... two-state assumption forthe unfolding reaction, and linear pretransition and post-transition baselines, which are dependent on the concentra-tion of guanidine hydrochloride by the equation Xo+ aX[guanidine ... divided into four parts (I, II, III and IV):I, interactions with amino acids 30–33; II, interactions with amino acids 33–34; III, interactions with amino acids 35–37; IV, interactions withamino...

Báo cáo khóa học: Nerve growth factor mediates activation of the Smad pathway in PC12 cells doc

... in multiple interdependent signalsbetween pathways originating from receptor serine/threo-nine kinases and receptor tyrosine kinases. Cellularresponses induced by bone morphogenetic protein(BMP) ... Identiﬁcation and functional characterization of a Smadbinding element (SBE) in the JunB promoter that acts as atransforming growth factor-b, activin, and bone morphogeneticprotein-inducible ... tyrosine kinase receptor; ECD, extracellular domain;MAPK, mitogen activated protein kinase; RSK, receptor serine/threonine kinase; BMP, bone morphogenetic protein; GFP, greenﬂuorescent protein.(Received...

Báo cáo khoa học: Amino acid residues on the surface of soybean 4-kDa peptide involved in the interaction with its binding protein potx

... Tager, H.S. (1987) Role of the COOH-terminal B -chain domain in insulin–receptor interactions:identiﬁcation of perturbations involving the insulin mainchain.J. Biol. Chem. 262, 12054–12058.20. ... protein;protein–protein interaction; alanine-scanning mutagenesis;surface plasmon resonance.A 43-kDa protein in legume seeds has been shown to bindto animal insulin [1]. This 43-kDa protein consists ... functions as a proteinkinase and controls cell proliferation and diﬀerentiation. Asthis peptide stimulates protein kinase activity, the interactionbetween the 4-kDa peptide (leginsulin) and...

Xem thêm