... 2006)doi:10.1111/j.1742-4658.2006.05249.xA novel elicitor that induces chitinases in tobacco BY-2 cells was isolated from Alternaria alternata 102. Six other fungi, including A. alternata IFO6587, could not induce, or weakly induce ... genes. In comparison with laminarin and laminarin sulfate, which are reported tobe potent elicitors of defense responses in tobacco, the expression patternof genes induced by the purified b-1,3-, ... PCR wasperformed with denaturing, annealing and extensiontemperatures of 94 °C for 0.5 min, 50–58 °C for 0.5 minand 72 °C for 0.5 min, respectively, for 24–34 cycles. Theannealing temperature...