... pre-pro-tyrosinase (Fig. S1) can be dividedapproximately into three domains: a twin argininetranslocase (TAT) signal peptide, a core domain con-taining the two copper -binding motifs and a C-termi-nal ... constructs without thepredicted N-terminal signal peptide (amino acids1–36), we retained amino acid 36, an alanine, ratherthan using amino acid 37, a lysine, because it isknown that, after a post-translational ... motifArg40Cys84Tyr349Tyr347Copper binding motifTrypsinisedpro-tyrosinase 332 amino acidsCore domain 36–370Copper binding motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet...