Báo cáo khoa học: Analyses of co-operative transitions inPlasmodium falciparumb-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding pdf

Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

... retinoid binding, and x-ray analysis of a human retinol -binding protein. Proc Natl Acad Sci USA 98, 3710–3715.5 Folli C, Calderone V, Ramazzina I, Zanotti G &Berni R (2002) Ligand binding and ... retinol -binding proteins (CRBPs) and the cel-lular retinoic acid -binding proteins (CRABPs). CRBPs,including CRBPI, CRBPII, CRBPIII and CRBPIV,show greatest binding afﬁnity for retinol and retinal,but ... genome.AbbreviationsCRABPIa and CRABPIb, cellular retinoic acid -binding protein type I a and b; CRBP, cellular retinol -binding protein; hpf, hours post fertilization;iLBP, intracellular lipid -binding protein; ...

Báo cáo khoa học: Analyses of co-operative transitions inPlasmodium falciparumb-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding pdf

... Plasmodiumfalciparum b-ketoacyl acyl carrier protein reductase upon co-factor and acyl carrier protein binding Krishanpal Karmodiya and Namita SuroliaMolecular Biology and Genetics Unit, Jawaharlal ... Plasmodium b-ketoacyl- acyl carrier protein reductase exhibits negative, homotropic co-operative binding for NADPH, which is enhanced in the presence of acyl carrier pro-tein. Acyl carrier protein increases ... the accessibility of NADPH to b-keto- acyl- acyl carrier protein reductase, as evident from the increase in theaccessibility of the tryptophan of b-ketoacyl -acyl carrier protein reductase to acrylamide,...

Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modiﬁcations and in enhanced protective activity in sepsis doc

... appearance of a number of novel antigen -binding speciﬁcities (Fig. 1B).Enhanced binding of low-pH buffer-exposed IVIgto recombinant human interferon-c (IFN-c) and other self-antigensInteractions of ... detectable binding to CRP at the concentration and during theperiod of observation used in the experiment.Interaction analyses using increasing concentrations of the native and of the low-pH ... others, that the in vitro exposure of monoclonal and of polyclonal IgG to various protein- destabilizing factors may result in dramatic enhance-ment of their binding polyreactivity. These treatmentsinclude...

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... for Protein Analyses and Targeted Protein Research Program of the Ministry of Education,Culture, Sports, Science and Technology of Japan(MEXT), by Core Research for Evolutional Science and ... system.Fig. 1. Comparison of 1H-13C constant-time HSQC NMR spectra of 0.6 mM of UL At3g16450.1 and 0.2 mM of SAIL At3g16450.1. (A) Fullspectrum of UL At3g16450.1. (B) Full spectrum of SAIL At3g16450.1. ... eachcomposed of three b-sheets. The fold of each domainis nearly identical to that of an agglutinin (Protein Data Bank code 1JOT), which shares sequence identi-ties of 26 and 33% with the N- and C-terminaldomains...

Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc

... amounts of protein forpurification. We also attempted to isolate the protein from the E. coli periplasm but could not find any evi-dence of activity, indicating a lack of export of the protein. ... cysteine-histi-dine thioether bridge in functional units of molluscanhaemocyanins and location of the disulfide bridges infunctional units d and g of the bC-haemocyanin of Helix pomatia. Eur J Biochem 248, 879–888.29 ... newexample of this interesting family of enzymes. In addition, we show thatthis enzyme can be readily overproduced and purified and that it will proveuseful in furthering the understanding of these...

Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt

... Howard S. Young3 and Kunio S. Misono11 Department of Biochemistry, University of Nevada, Reno, NV, USA2 Department of Chemistry, University of Nevada, Reno, NV, USA3 Department of Biochemistry, ... ﬂuorescence studies of Trpresidues, taking advantage of the fact that Trp74occurs at the hh interface and that its local environ-ment changes upon ANP binding, whereas the envir-onment of other Trp ... thesolution structures of apoECD and ANP–ECD.The ﬂuorescence emission spectra of apoECD and ANP–ECD are shown in Fig. 4C. Comparison of thespectra shows that addition of ANP causes an approxi-mately...

Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

... Site-directedmutational analysis for the ATP binding of DnaA pro-tein. Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP -binding domain of DnaA protein in vitro and in vivo. J Biol Chem ... Mg2+ and ATP concentrations during electropho-retic separation, may inﬂuence binding, and we thinkthis the more likely explanation; direct comparison of the ATP dependence of EMSA and binding of ... withrecombinant protein, and this difference may arisefrom different characteristics of native HepG2 protein and recombinant protein, such as the presence of aHis-tag on the latter. Although ATP-cS is often...

Báo cáo khoa học: Inhibition of the NF-jB transcriptional activity by protein kinase A pot

... pGal4-CREB (1–280),pCRE-luc containing four tandem repeats of the CREB- binding sites, pGal4-luc containing ﬁve tandem repeats of Gal4 -binding sites, and the mammalian expression vectorpCMV-PKAc ... conditions and luciferase assayTransient transfection of Jurkat T-lymphocytes wasaccomplished by using 5 lL of SuperFect transfectionreagent (Qiagen) per ml of culture medium and a total of 1 lg of ... mMNa3VO4,1mMphenylmethane-sulfonyl ﬂuoride, and 2.5 lgÆmL)1each of aprotinin,leupeptin and pepstatin). Equal amounts of samples(20 lg of total protein) were subjected to 10% SDS/PAGE and transferred onto a nitrocellulose...

Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... stabiliza-tion of labile drugs, control of volatility and sublimat-ion, physical isolation of incompatible compounds,long-term protection of color, odor and ﬂavor, and sup-pression of hemolysis and the ... pockets of a-CD and b-CD are not able to form stable complexes withGA, the binding of a-CD to GA is not observed and that of b-CD is much weaker than that of c-CD. Thedifference in binding ... dif-ferent conditions of pH, buffer and temperature. Inaddition, NMR and DLS analyses demonstrated thecharacteristic properties of GA. Our results of themolecular interaction between GA and c-CD can...

Báo cáo khoa học: Selection of full-length IgGs by tandem display on ﬁlamentous phage particles and Escherichia coli ﬂuorescence-activated cell sorting screening doc

... wereexpressed and purified by protein A affinity chroma-tography, and IgG in yields of 0.5–3 mgÆL)1wereobtained. Biacore analysis of binding kinetics revealedthat the affinities of the IgGs derived ... highest-affinity and lowest-affinity IgGswere determined to have KDvalues of 1 and 440 nm,respectively.Enrichment of high-affinity and moderate-affinityIgGsTo evaluate the utility of tandem phage ... establishment of anIgG phage display system, we took advantage of thefUSE5–ZZ phage. These phage particles display theFc -binding ZZ protein on all five copies of the phagep3 coat protein, and are...

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