Báo cáo khoa học: IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain pdf
Báo cáo khoa học: IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain pdf
... 2006 The Authors Journal compilation ª 2006 FEBS IMP1 interacts with poly(A)-binding protein (PABP) and the autoregulatory translational control element of PABP-mRNA through the KH III-IV domain Gopal ... PABP and IMP1 interact with each other, regardless of the presence of the ARS, through the conserved C-terminal PABP-C and K-homology (KH) III-IV domains,respectively. Interaction of PABP with the ... PABP and the PABP-C peptide (Fig. 7D:lanes 6 and 7). Furthermore, the IMP1 KH III-IV pep-tide was also able to pull down the full length IMP1, and IMP1 peptides containing the KH III-IV domains(Fig....
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Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc
... N-acetylated and neutralcarbohydrates [8].It is synthesized in the liver and, like the other ficolins, has the potential of initiating the MBL ⁄ lectin complement cascade through the MASPs [12–15].SP-A ... [12–15].SP-A and SP-D are lung surfactant proteins. LikeMBL and the ficolins they are pattern-recognitionmolecules but do not share the property of binding the MASPs and therefore do not activate the ... satura-ble. The interaction between CD91 and MBL and CD91 and L-ficolin could be inhibited by ligands of both CD91 (RAP, C1q) and MBL (MASP-3) and therefore fulfilled the requirements of a specific...
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Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... encoded the full-lengthcDNA of the calcium and integrin binding protein (CIB) inframe with the GA L4 AD.Mapping of regions involved in the interactionshow that CIB and FEZ1 bind in the same domain of ... sequencesimilarity with the m urine homologue. The murine Nbr1 geneliesheadtoheadwiththeBrca1 gene, with the intragenicregion of 289 bp suggesting the possibility of co-ordinatedexpression of the two genes. ... O-glycosylated protein c orrelate with the expected p roperties of the polypeptide carrying the CA125 epitope [4].NBR1 shares little h omology with other k nown proteins,but does contain a number of protein...
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Báo cáo khoa học: Nucling interacts with nuclear factor-jB, regulating its cellular distribution potx
... the regulation of the activation of NF-jB following treatment with TNFa.According to the standard model of classical NF-jBsignaling, the activation of NF-jB is promoted by the degradation of ... Research and a Grant for the 21stCentury COE Program from the Ministry of Educa-tion, Science, Sports and Culture of Japan for the Promotion of Science, and a Research Grant from the Ministry of ... In the pres-ent study, a weak band of COX2 protein was observedin WT MEFs. We considered that the expression of COX2 was induced by the presence of nuclear NF-jB-p65 in WT MEFs in half of the...
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Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
... that the gene nor-mally encodes a putative protein of 157 amino acids with a predicted molecular mass of 18.2 kDa. Interro-gation of the NCBI nucleotide database revealed the identity of A1 with ... combinations of Table 1. Isolation of Rp-interacting proteins in yeast. The number of colonies obtained (clone A1 to A37), their growth on various selectivemedia (SM), and their production of a- and ... transformed with the combination of plasmids shown. The growth of single yeast colonies containingthese plasmids are shown in (B) and (E) onSD plusL-histidine and adenine and (C) and (F) on...
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Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc
... acids of the core domain and completeheadpiece domain mediated binding with the DH domain of human Ras-GRF2 (Fig. 1). In contrast, a bait constructcontaining only the headpiece domain of dematin ... cytoskeleton.Elucidation of the crystal structure of tandem DH a ndPH domains of human Sos1 protein highlights the dramaticcomplexity of the DH domain mediated interactions [35]. The c rystal structure ... cleave the dematin(224±383) domain from the GST fusion p rotein.A homogeneous sample of the dematin(224±383) (free of the GST domain) was immobilized ( 1.0 ng of protein permm2 of surface) to the...
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Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt
... 7Spre-rRNA, and as Nop53p interacts with the exosomesubunit Rrp6p and activates the RNase activity of the complex in vitro, Nop53p may be involved in recruit-ment of the exosome to the 7S pre-rRNA ... two-hybrid analyses and into YCplac33GAL-A, fusedto the protein A tag, under the control of the GAL1promoter [31]. Subsequently, the fragments coding for the N- and C-terminal regions of NOP53 were ... of 7S, a phenotypesimilar to that caused by the depletion of core exo-some subunits [31]. Nop53p interacts with the nucleo-lar proteins Nop17p and Nip7p [31], both of whichinteract with the...
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Tài liệu Báo cáo khoa học: Amprenavir complexes with HIV-1 protease and its drug-resistant mutants altering hydrophobic clusters docx
... bonds to the carbonyl of Gly27 and the amide of Asp29 and a H2OÆÆÆp interaction with the ani-line group of APV. The interactions of H2OBare con-served in PR complexes with DRV and antiviralinhibitors ... contacts with the side chains of Val56,Leu76 and the main chain atoms of residues 77–78, and Ile32¢ showed new interactions with the side chains of Ile47¢, Ile50 and Val56¢ in the flaps (Fig. 3A). The flaps ... C-HÆÆÆO interactions with the hydroxyloxygen of Thr80¢ and carbonyl oxygen of Pro79¢, and the Cd1 of Ile50¢ interacts with the hydroxyl of Thr80.Mutation from Ile50 to Val shortens the side chain...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... interacts with the carboxy group on the substrate and con-trols the preference for carboxylated and decarboxylated substrates. The second is the size of the residue isosteric with the aromatic ... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS.Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor,L-dopa. ... but the differentiation between these two types of enzymeis not clear [4]. Looking at the sequences of the twoenzymes, both show absolute conservation of the histi-dine residues of the CuA and...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
... test the interaction of Nop53p with other proteins. (A) Test forpositive interactions between Nop53p and other proteins fused to the Gal4p activation domain (AD), or to the lexA DNA binding domain ... colocalizes with RFP–Nop1p The interaction of Nop53p with Nop17p, a nucleolar protein [28], and Nip7p, a protein that localizes to the nucleus and the cytoplasm [9], raised the question of where ... data, the authors found that the depletion of Nop53p has stronger effects on the maturation of the 25S rRNA, and not on the 5.8S.Our data show that Nop53p coprecipitates the 27S and 7S preRNAs and...
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