Báo cáo khoa học: Diversity of human U2AF splicing factors Based on the EMBO Lecture delivered on 7 July 2005 at the 30th FEBS Congress in Budapest pptx

... 305–308. U2AF diversity I. Mollet et al.4816 FEBS Journal 273 (2006) 48 07 4816 ª 2006 The Authors Journal compilation ª 2006 FEBS THE EMBO LECTURE Diversity of human U2AF splicing factors Based on the EMBO ... domains in relation to U2AF 65is indicated. (B) The putative functional domains in each proteinare aligned with U2AF 35, and the similarity(% identity) of these domains in relation to U2AF 35is ... gene revealed that the exon ⁄ intronboundaries are located in the same positions as in the human U2AF1 gene, although the introns are much U2AF 65 U2AF 35 U2AF 26 U2AF 35R1 U2AF 35R2PUF60CAPERαCAPERβFig....

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... junction.Conﬁrmation of utilization of exon 1a asa transcription initiation exon and occurrence of alternative splicing To examine whether exon 1a is used as a transcriptionstarting exon in QGP-1 ... or in vitro, including characterization of the promoterregion of the gene and the associated transcriptional factors. Therefore, delineation of the transcriptionalregulation of human DNASE1 may ... indicates the splicing junction betweenexon 1a and a portion of exon 1. The thick straight lines represent the intron sequence. +1 indicates the position of the transcriptionstart site of exon...

Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

... partialdisulfide bonds. Two conventional methods are often used in the investigation of disulfide bond-containing proteinunfolding. One such method is denaturation, which requires the use of denaturants ... vitro. Intermediates are namedusing the disulfide bonds they contain. Arrows with dashed linesindicate the folding pathway for the first formation of the intra-Adisulfide bonds. Another major folding ... have investigated the guanidine hydrochloride-induced equilibrium denaturation of insulin and proinsulin[29,30]. The results of previous work on insulin areconsistent with a two-state denaturation...

Báo cáo khoa học: ` Inhibition of human ether a go-go potassium channels by 2+ Ca ⁄calmodulin binding to the cytosolic N- and C-termini potx

... under control conditions, i.e. with Ca2+-free bath solu-tion, upon application of the indicated concentrations of hCaM in the presence of 1 lM free Ca2+solution, and after washing off the CaM ... 3A–C)coding for the putative binding sites in hEAG1. In the presence of 25 lm free Ca2+, the aspartate-to-alanine mutations in the N-terminal half of CaM(hCaM-EF12) increased the dissociation constant ... one domain in the N-terminus of hEAG1. The latter one bindsCaM only in the presence of Ca2+and is functional in a sense as point mutations in this domain abolishedCa2+-sensitivity of the channel....

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... permeabilization induced by the proteinswas expressed as a percentage of the maximal permeabiliza-tion obtained at the end of the assay by the addition of Triton X-100 to a ﬁnal concentration of 2 ... 17. 6Fig. 5. Insertion of steﬁns into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into ... thatinteraction with the monolayer induces cooperativeconformational rearrangements or further oligomeriza-tion on the surface of the monolayer. The increase in pressure was measured as a functionof...

Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx

... contribution of the cellular kinases in tissues and is oftendetermined using only one saturating concentration of eachsubstrate. Obviously, choosing a high substrate concentra-tion, as one usually ... iscorrelated to the presence of the 2¢-OH of the sugar in the cisposition. The latter is absent in dCMP andL-3TCMP, andis in trans in the arabinose epimer. The nonproductivebinding of NMP ... according to the conformation in the complex withthekinaseasfoundin3UKD.PDB.b-L-3TCMPwaspositionedintheactive site by choosing the same orientation for the cytidine basemoiety as in the complex...

Báo cáo khoa học: Comparison of human RNase 3 and RNase 7 bactericidal action at the Gram-negative and Gram-positive bacterial cell wall pot

... a bin-ocular microscope at ·50 magniﬁcation. The agglutinatingactivity is expressed as the minimum agglutinating concen-tration of the sample tested, corresponding to the ﬁrst con-dition ... al. 171 4 FEBS Journal 277 (2010) 171 3– 172 5 ª 2010 The Authors Journal compilation ª 2010 FEBS in the present study therefore contributes towards elu-cidating the main determinants of their distinct ... RNase 7 bactericidal activity FEBS Journal 277 (2010) 171 3– 172 5 ª 2010 The Authors Journal compilation ª 2010 FEBS 172 5Comparison of human RNase 3 and RNase 7 bactericidalaction at the Gram-negative...

Báo cáo khoa học: Overexpression of human histone methylase MLL1 upon exposure to a food contaminant mycotoxin, deoxynivalenol docx

... addition, the level of Sp1 is enriched in the Sp1-binding regions of the MLL1 promoter upon exposure to DON. These resultsdemonstrated that Sp1 acts a mediator in translating the effects of DON on ... demonstrated that DON-induced MLL1 and HoxA7 gene upregulation werealleviated in the presence of PP2. These observationsdemonstrated that Src kinases are involved in upstream events in DON-mediated ... Rbbp5 (data not shown), suggesting the involvement of alternate pathways in the regulation of these genes.Our sequence analysis demonstrated that the MLL1promoter contains multiple binding sites...

Báo cáo khoa học: Diversity of metallothioneins in the American oyster, Crassostrea virginica, revealed by transcriptomic and proteomic approaches potx

... as either a or b, based on the number and conﬁguration of the cysteine motifs. The Ônoncoding exonÕ region represents the b-domain region of the transcript that hasbeen truncated by the introduction ... members. Initially, the mutation of a lysine codon toa stop codon in the linker region would have truncated the MT protein after the a-domain. Subsequent tandemduplications of the a-encoding sequence ... (1,2)intron phase. The three genes differed remarkably in theirintron lengths. The ﬁrst introns in the two CvMT-I geneswere the only ones of similar length. The second intron wasmuch shorter in...

Báo cáo khoa học: Inhibition of human MDA-MB-231 breast cancer cell invasion by matrix metalloproteinase 3 involves degradation of plasminogen docx

... tumour cellinvasion in vitro by a mechanism involving plasminogendegradation to fragments that limit plasminogen activationand the degradation of laminin. This supports the hypothesisthat MMP-3, ... components and activates MMPsresulting in further ampliﬁcation of BM degradation[ 17 21]. Amongst the MMP family, MMP-3 (stromelysin-1)is considered pivotal to the integration of plasmin andMMP ... that inhibitors of plasminactivity, including the anti-catalytic anti-plasmin antibodyused in this study, abrogate the invasion stimulating effects of plasminogen upon MDA-MB-231 cells, conﬁrmingdependence...

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