... and the analysis of their binding activity to the receptor toinvestigate the role of mutated amino acid residues; (b) the analysis of the influence of the modification of the cysteine residues of ... BtR175From the results of the IAsys binding analysis, the cys-teine substitutions at R281, Q293 and G442 did notseem to influence the conformation of Cry1Aa toxins,as the binding abilities of these ... IAF-modified forms, similar toG442C. In addition, the binding curve of DF-boundY445C was the same as that of the nonmodified form,which showed lower affinity (data not shown). Analysis of the binding...