Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

... Merck and Co., Inc. Journal compilation ª 2007 FEBS 1803 Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella ... 2007)doi:10.1111/j.1742-4658.2007.05728.x A highly active glutathione S-transferase was purified from adult German cockroaches, Blattella germanica. The purified enzyme appeared as a singleband of 24 kDa by SDS⁄ PAGE, and had a different ... classes of cytosolic GSTs in insects [2]. The majority of GSTsare in the Delta and Epsilon classes, and the remain-ing enzymes are in the Omega, Sigma, Theta and Zetaclasses. The German cockroach...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... [34]. An evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinitychromatography as it gave a higher fold of purification and percentage ... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. ... SDS/PAGE gave a singleband at apparent molecular mass of 34 kDa. The binding affinity of the lectin in the hemolymph of the freshwater crab, Paratelphusa jacquemontii, expressedO-acetyl sialic acid...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the polypeptides of the puriﬁed enzyme. N-Terminal sequen ... 3 bp). The region 81–65 bp upstream of t he start c odon of AF499 was identiﬁed asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity ... proteincomplex then is translocated across the cytoplasmic mem-brane via the TAT translocase. Such a mechanism has beenfound for periplasmic oxidoreductases [27]. The characterization of the A. fulgidus...

Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

... in the biosynthesis of methylated dianthramide-derivatives, the carnation phyto-alexins. However, no data are available regarding the role of this enzymatic activity in the biosynthesis of methylatedphenols ... activity was weak and did not change statisticallyalong the 72 h of the observation period. A statisticallysigniﬁcant increase of the F 4¢-OMT activity could berecorded in the same observation ... compo-nents of each reaction mixture, after their chromatographicseparation and puriﬁcation, were submitted to1HNMR(nuclear magnetic resonance) and FABMS (fast atombombardment mass spectrum) analyses...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon doc

... number AI563351) was used to design fourGAT-speciﬁc primers; CLGAT 5a (5¢-GGCATCAACAT-CACAAGCAACAAGTGCAAG-3¢), CLGAT5b (5¢-TCCT-CCATCAATCTGCTTCCATGGACCATC-3¢), CLGAT 3a (5¢-GCTGTGGCTACGAATGAGGCCGCC-3) ... FEBS Puriﬁcation and characterization of glutamateN-acetyltransferase involved in citrulline accumulationin wild watermelonKentaro Takahara, Kinya Akashi and Akiho YokotaGraduate School of ... NotI-d(T)18bifunctional primer (5¢-TAA-CTGGAAGAATTCGCGGCCGCAGGAAT(18)-3¢). The single-stranded cDNA was used for PCR w ith the primers Not1(5¢-AACTGGAAGAATTCGCGGCCGC-3¢) and CLGAT 3a. An aliquot from t...

Báo cáo khoa học: Puriﬁcation and characterization of zebraﬁsh hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... forward: 5¢-CTGAACTTCTCTACACACTGAGG-3¢, reverse: 5¢-CCTTATCACCATCACCTCACTTC-3¢; ZHE2 forward: 5¢-CTCCACACACTGAGACTAAATGG-3¢, reverse: 5¢-GGAAATAAGAGCACGTACTGTGG-3¢. The cycle number of PCR was ... using a sense primer, 5¢-CATATGAATGCTCTCATCTG CGAGGACA-3¢, containing a 5¢ NdeI site and start methionine resi-due, and an antisense primer, 5¢-GGATCCTAGTGATGGTGATGGTGGCATCCATACAGCTTATTGATCC-3¢,which ... Maruyama K, Kawazu K, Kaneko T, Ohtani-Kaneko R & Yasumasu S (2004) Structure and develop-mental expression of hatching enzyme genes of the Japanese eel Anguilla japonica: an aspect of the evolution...

Báo cáo khoa học: Puriﬁcation and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... translation showed that they hold the characteristic features of all known papain -class cysteine proteinases, and a phylogenetic analysis revealed the existence of several papain and chymopapain ... commonancestor of the genera Carica and Vasconcelleaalready contained at least two different cysteine pro-teinases in its latex (papain and chymopapain). After the divergence of these genera, their ... Inaddition, it has been demonstrated that repeatedmechanical wounding of the fruit profoundly affectsTable 1. Protein concentration and amidase activity of latex of Vasconcellea and C. papaya fruits.SpeciesProteinconcentration(mgÆg...

Báo cáo khoa học: Puriﬁcation and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx

... Hiroko Saika, Keizo Yuasa and Masami NagahamaDepartment of Biological Science and Technology, University of Tokushima Graduate School, JapanGenome analysis has indicated that plants, likeanimals, ... most of the activity was lost. CBCP had optimal activity at40 °C. The activity of the puriﬁed enzyme was alsoexamined against various synthetic substrates and compared with that of human cathepsin ... cotyledons obtained 8 days afterimbibition. From cotyledons obtained 3 days afterimbibition, a faint 28 kDa band and additional bandswere also detected. A clear 28 kDa band and addi-tional bands were...

Báo cáo khoa học: Puriﬁcation and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily doc

... arginase and H. pyloriarginase-containing extracts with divalent cationsMammalian arginases require manganese for optimalcatalytic activity, although some of these arginases may beactivated ... puriﬁed and characterized,most are from the genus Bacillus; like eukaryotic arginases,these prokaryotic arginases were found to have an optimalcatalytic activity with manganese and showed a highlyalkaline ... at )20 °C until measurement of arginase activity. No arginase activity was detected in the correspondingpellets.Arginase activity assay The colorimetric arginase assay measures the amount of ornithine...

Báo cáo khoa học: Puriﬁcation and characterization of NTPDase1 (ecto-apyrase) and NTPDase2 (ecto-ATPase) from porcine brain cortex synaptosomes doc

... changed their afﬁnitytowards ATP and ADP [11–15].NTPDase1 differs from NTPDase2 in respect to reactionproducts of ATP hydrolysis and in the ratio of the rate of ATP hydrolysis to the rate of ... conditions and in a 10% acrylamide SDS gel(Figs 1, 2A) . Ion exchange chromatography on a MonoQHR 5/5 column was a crucial step of puriﬁcation and separation of synaptosomal NTPDases. Chromatographyon ... 5 lm) and computer softwareCHROMA from PolLab (Warsaw, Poland). MonoQ HR 5/5 column wasobtained from Pharmacia. Nitrocellulose membrane NC2was obtained from Serva. Silver stain kit and BCIP/NBTfast...

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