... monomeric forms of native collagen types I and
III at 37 °C and 25 °C, respectively. Activity against collagens I and III
was abolished by metalloproteinase inhibitors and was not present in the
concentrated ... demonstrating specific cleavage of native
type III collagen with this enzyme (Fig. 4, type III, T)
[27]. The more extensive digestion of denature...
... matrix binding properties of recombinant
fibronectin type II-like modules of human 72-kDa
gelatinase ⁄ type IV collagenase. High affinity binding
to native type I collagen but not native type IV ... internalization and
degradation.
Role of exosites in regulation of activity
The complex substrate specificity of individual MMPs
is not only determined by their substrate-bind...
... zinc ion, peptidomimetis are subdivided into
Table 2. Potential matrix metalloproteinase inhibitors.
MMPI Type of drug ⁄ source Enzymes inhibited
Synthetic inhibitors
Batimastat Peptidomimetic ... generation of MMPIs, tetracycline derivatives,
inhibit both the enzymatic activity and the synthesis of
MMPs via blocking gene transcription. Chemically
modified tetracyclines, lacking an...
... the similarity with animal insulin in binding
the basic 7S globulin and stimulating its protein kinase
activity [16], the 4 kDa soybean peptide was initially
designated leginsulin [12] but this ... a
cross-kingdom bioactive peptide family. Interestingly,
similarity searches using the Swiss Institute of Bio-
informatics (SIB) BLAST network service (http://
www.expasy.org/cgi-bin/blast.pl...
... used in this work are listed in Table 5.
Split-ubiquitin technique
For the split-ubiquitin interaction assays, 10
5
wild-type cells
carrying the split-ubiquitin plasmids were spotted onto
SC-His ... proximity. This
reconstituted quasi -native ubiquitin is recognized by ubiquitin-
specific proteases (USPs), which cleave off the reporter RUra3,
which is fused to the PAK. Released RUra3, a modifi...
... Angelo Vianello
2
1 Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole, Universita
`
di Trieste, Italy
2 Dipartimento di Biologia ed Economia Agro-Industriale, Sezione di Biologia Vegetale, ... bilirubin, biliverdin, section B) and in Table 2 (K
d
of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin
3-glucoside, sections C and D).
A Michaeli...
... Choedchai Saehuan
2,
* and
Michael J. McLeish
1
1 Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, IN, USA
2 Department of Medicinal Chemistry, University ... when alternative pathways are avail-
able, it is unlikely to play any significant role in the
catabolism of the branched-chain amino acids or of
methionine.
Identification and...
... C-terminal region of GRIP1 is involved in
the repression of transactivation activity and self-
association of GRIP1 (Figs 1–5), we examined the
relationship between transactivation and co-activation
of ... 2183
Modulation of glucocorticoid receptor-interacting
protein 1 (GRIP1) transactivation and co-activation
activities through its C-terminal repression and
self-associat...
... xtracellular
matrix could occur through the Fas I domains or putative
GPI unit, if one exists in CLAP as it does in Drosophila
fasciclin I and many other fasciclin-containing proteins
[36,46]. Fas I domains in ... the
dimer retained about 70% of its initial activity under these
conditions (Fig. 4C). Similar differences in cathepsin L
activity were observed at a ll incubation t...
... to virus infection induces sufficient
apoptosis to limit the levels of virus multiplication,
thus mimicking an antiviral effect. An alternative
mechanism, that spermine acts by inhibition of virus
entry, ... ArgII expression was IFN-independent
(Fig. 3).
ArgI and ornithine decarboxylase (ODC) are not
induced in response to virus infection
As the two isoforms of arginase, I (hepatic i...