... article.
PACSIN 1 oligomerization A. Halbach et al.
782 FEBS Journal 274 (2007) 773–782 ª 2007 The Authors Journal compilation ª 2007 FEBS
PACSIN 1 forms tetramers via its N-terminal F-BAR domain
Arndt ... of PACSIN 1 with an N-terminal F-BAR
domain and a C-terminal SH3 domain. The
F-BAR domain was formerly known as the
CDC15-NT domain, and includes a con...
... B-II: 0 .1% SDS, 1% Triton X -10 0, 2 mm
EDTA, 500 mm NaCl, 20 mm Tris ⁄ HCl, pH 8; B-III:
0.25 m LiCl, 1% Nonidet P-40, 1% sodium deoxycholate,
1mm EDTA, 10 mm Tris ⁄ HCl, pH 8; B-IV: 1 mm
EDTA ,10 mm ... containing 1% SDS, 10 mm
EDTA, 50 mm Tris ⁄ HCl, pH 8. Lysates were centrifuged at
14 000 g for 10 min at 4 °C, and the supernatant obtained
was diluted 10 -fold using IP buffer...
... 7 8 910 111 213
PDI
P2X
7
R
-Cop
Flotillin -1
B
Triton X -10 0
soluble insoluble
P2X
7
R
Cav -1
Flotillin -1
T1
TfR
Fig. 2. (A) Solubility of Caveolin -1, Flo -1 and P2X
7
receptor in Triton X -10 0. E10 ... Caveolin -1 protein in E10
cells, we designed two different shRNAs targeted
to the mouse Caveolin -1 mRNA. Figure 6B shows
Cav -1
12345678 910 111 213 14
P2X
7
R
Flotillin -1
-Co...