Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf

... FEBS Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum Deborah K. Shoemark1, Matthew J. Cliff2, Richard B. Sessions1and Anthony R. Clarke11 Department of ... polarity of the environment of the ﬂuorophore. In the case of PfLDH, the signal changeon binding to the active site was too small to be usedas a reliable reporter of the formation of the binarycomplex. ... the rate of the conformationalchange, k3H the hydride transfer and k4 the rate of the product-off step. Hence, in these circumstances, the steady-state KMis expected to be smaller than the apparent...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... tetramers from the hexamer wasconsidered to result from direct action of the enzymes.On the other hand, the pentamer appeared to beformed by the condensation of smaller intermediates.Note that the ... (GlcNAc)3+(GlcNAc)5. The rates of forma-tion were in the order of (GlcNAc)6>(GlcNAc)5> (GlcNAc)8for both enzymes. The ratios of the maximal yields of the synthesized productsobtained by the mutant ... ESIMS, the kinetic properties of chitinase A with pNP-glycosides, chitooligosaccharides, and chitin. The ini-tial velocity of the enzyme for concentrations of the substrates ranging from 0...

Báo cáo khoa học: Enzymatic investigation of the Staphylococcus aureus type I signal peptidase SpsB – implications for the search for novel antibiotics ppt

... and therefore the FRET assay was used tostudy the effect of pH on the enzyme. The stability of the synthetic SceD peptide substrate was determinedby incubating it in different buffers in the ... using these assays. This line of research has the potential to result in a new class of Fig. 7. A comparison of the activities of the full-length and the truncated SpsB derivatives. (A) The enzyme ... analysis of secreted proteins indicated in the genomic sequence data of S. aureus. The criteriafor selection of the substrate were a good prediction of the presence and location of the signal...

Báo cáo khóa học: Structural properties of the protein SV-IV potx

... identiﬁed by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... and the equilibrium of self-association appears to control the biological properties of the protein [7]. Moreover, the immunomodulatoryactivity is related to the structural integrity of the ... by the addition of 5.4 mMSDS (Fig. 1), whereas in the 1–70 region, the a-helixcontent increases from 2–3% to  23% in the presence of SDS (data not shown). This suggests that the effect of...

Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... changing the activity of the enzyme by x%results in a signiﬁcant change of the pathway ﬂux byat least 0.5x% (whether 0.5x% or higher is a matter of convention). The way that the change of enzyme ... iscapable of reducing the level of fructose 2,6-P2inde-pendent of the phosphorylation state of iPFK-2.Reducing the level of fructose 2,6-P2and thus the activity of PFK-1 improves the supply of ... produc-tion of (neurotoxic) lactate [58]. Such a hypotheticalfunction of HK-2 can hardly be reconciled with the notion of excessive lactate production being the ulti-mate goal of the Warburg...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... con-served in the primary structure of the GTPase domain of hGBP5, so the formation of GMP must be impairedin an as yet unknown manner. Sequence alignments of the large GTP-binding domains of hGBP1, ... for the lack of GMP formation(see Fig. S2). The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are incontrast to that of the analogous deletion mutant of hGBP1 ... manner, the entropic contributions of nucleotide binding and the coupled oligomerizationshow opposing signs. In the case of hGBP1, release of water from the nucleotide binding pocket and the pro-tein...

Báo cáo khóa học: Functional properties of the protein disulﬁde oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulﬁde-isomerase doc

... sites. Refolding of the scrambled RNase in the presence o f P DI was used as apositive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus ... wasusedasanegativecontrol. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efﬁcient when usingPDI. However, the temperature of the assay, which islimited by the stability of the protein ... (225 mM)9:1(25 mM). At the end of the incubation, 40 lL of the mixturewas added to each of the ﬁve solutions; these were then leftto react on ice for 5 min. The reaction mixtures w ereanalysed...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... the superimpo-sition of ApeSOD with the cambialistic SOD from P. shermanii. The overall structures of these enzymeswere similar; the rmsd of the 747 Ca atoms was0.695 A˚.ApeSOD eluted from ... ligands. The additional water occupied the postulated superoxide bind-ing site. The thermal stability of the enzyme was compared with superoxidedismutase from Thermus thermophilus, a thermophilic ... ligand,NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A˚out of the equatorial plane(Table 3). The angles around the metal...

Báo cáo khoa học: Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase doc

... responsible for the change of the oligomeric state.These data point to an essential function of the small subunit of the decarboxylase and may indicate unique regulatory properties of the system.Keywords: ... allowin-frame cloning of the genes. While the BamHI sitesintroduced in the 3¢-UTR of the genes did not affect the resulting amino acid sequences, the introduction of NheIorNcoI sites next to the start ... 0.5 UÆmg)1). The recombinant decarboxylase eluted from the anionexchange column in a similar position to the enzyme from C. diﬃcile. In contrast, the behaviour of this enzyme on the size exclusion...

Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... strain X-33 with the addition of asignal sequence for secretion of the recombinant pro-tein into the medium. Approximately 12 mg of the recombinant lectin was puriﬁed from the medium of a1 L culture ... incomplete process-ing of the signal peptide was also reported [16,17]. The uncleaved part of the a-mating sequence at the N-ter-minus as well as the histidine tag at the C-terminus of the recombinant ... Foundation and the Center of Excellence project (PF 10 ⁄ 08) of the K.U. Leuven.Bassam Al Atalah is recipient of a doctoral grant from the Special Research Council of Ghent Univer-sity. The authors...

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