Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... speci-
fic roles of the various members of the DIM family,
e.g. DIM A and DIM B, in these functions have never
been investigated. The recent developments in our
understanding of the biosynthetic ... between the number of CFUs of
the wild-type strain and that of the PMM56 mutant
strain in lungs and in the spleen (Fig. 7A). Indeed,
both strains multi...
... base-pairs in the centre of the junctions [19]. Detailed
understanding of the functional role of these interactions
would require the crystal structure of the Ydc2–junction
complex.
In this study the ... [14,20]. Binding of CCE1 results in the
complete unpairing of the four base-pairs in the centre of
the junctions with a lesser disruption of the...
... least for the
Fig. 2. Schematic view of the parallel
between Pin1 and CKS in protein degrada-
tion. Top: Model of the SCF
CDC4
E3 ligase
and the role of Pin1. Pin1 is brought in with
the cyclin E ... similar binding
pockets of Pin1 and FKBP and the structural resem-
blance between their respective high-affinity inhibitors
[28] further underscore the si...
... were
subcloned into pBSSK (Stratagene). The structure of the
gene including the 5¢ and 3¢ flanking regions was determined
by double-stranded DNA sequencing, exons were identified
according to the sequence of ... sites within the MGP coding sequence
(Fig. 2 ). Analysis of the phase of each of the xMGP introns
located within the coding region revealed that introns 2 a...
... structures of the
wild-type, single- and double-mutant EhPGKs in a
closed conformation will certainly help to clarify the
molecular arrangement in the guanine ring binding site
during the binding of ... B);
and Val339, Gly340 and Val341 (patch C).
The corresponding reported amino acid residues
involved in adenine binding in the other crystallized
proteins, as w...
... gain insights into the structural dynamics of the DNA-binding
domains of two prototypic strains, human papillomavirus strain 16 and the
bovine papillomavirus strain 1. The simulations underline ... to
alanine of the residues Lys325 and Lys327, located in
the b2–b3 loop, produce a decrease in the DNA bind-
ing [22] that is restored upon back mutation into
arginine,...
... B
(transactivation domain), C (DNA-binding domain),
D (hinge region) and E ⁄ F (ligand- or hormone-binding
domain) [8,9]. The X-ray crystal structures of the
ligand-binding domains of EcR, USPs and RXRs have
been ... specific binding of LaEcR-A was not increased in
the presence of LaRXR. These results are in contrast to
the insect receptors where the specific binding...
... [12,14,15,19,20,22],
there are no reports on the true nature of the RGD-
containing proteins and their interacting partners.
The involvement of the PLDa C2 domain in these
Fig. 7. C2 domain is degraded by cardosin ... at the
tip of a loop protruding away from the core of the
protein. This amino-acid motif mimics RGD in terms
of charge and is positioned at th...
... linked, and incorrect
binding of ATP can affect serine binding and vice
versa [22]. Asn435, together with Asp416 and Glu432,
contributes to binding of the Mg
2+
ion between the
a- and b-phosphates of ... r.m.s.d of C
a
atoms after superposition of the
catalytic domains in complex with different substrates and the cata-
lytic domain of the apo-enzyme. The...
... strains
revealed the presence of a single point mutation in
1337 nucleotides of BmIVD. In the sku mutant, the
1127th guanine from the start codon was substituted
by thymine and none of the other sites ... acts in the third step of leucine degradation and the
sku mutant accumulates branched-chain amino acids in haemolymph, this
mutant may be useful in the...