Báo cáo khoa học: Structural requirements for antimicrobial versus chemoattractant activities for dermaseptin S9 pptx

... al. Antimicrobial and chemotactic dermaseptin S9 FEBS Journal 275 (2008) 4134–4151 ª 2008 The Authors Journal compilation ª 2008 FEBS 4151 Structural requirements for antimicrobial versus chemoattractant ... Drs S9 has formed self-associ-ated oligomers with potent antimicrobial activity. D7, after 7 days, Drs S9 has formedamyloid-like ﬁbrils.C. Auvynet et al. Antimicrobial and chemotactic dermaseptin ... 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol; Drs B2, dermaseptin B2; Drs B4, dermaseptin B4; Drs S9, dermaseptin S9; ERK, extracellular signal-regulated kinase;fMLP, formyl methionyl-leucyl-phenylalanine; FPRL-1, formyl peptide...

Báo cáo khoa học: Structural requirements for Caenorhabditis elegans DcpS substrates based on ﬂuorescence and HPLC enzyme kinetic studies pdf

... Journal 277 (2010) 3003–3013 ª 2010 The Authors Journal compilation ª 2010 FEBS 3013 Structural requirements for Caenorhabditis elegans DcpSsubstrates based on ﬂuorescence and HPLC enzymekinetic ... HIT motif iscritical for catalysis [14,20]. This histidine is involvedin the formation of a covalent nucleotidyl phosphohist-idyl intermediate, the nucleophilic agent for thec-phosphate group ... method. The Michaelis–Menten curves (vo versus co) obtained for these compounds are presentedin Fig. 3.The initial velocity data showed that the kinetics for MMG and TMG caps were hyperbolic...

Báo cáo khoa học: Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) potx

... complex. Science 284,812–815.1876 A. T. Nies et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Structural requirements for the apical sorting of human multidrugresistance protein 2 (ABCC2)Anne T. Nies1,Jo¨rg ... °Cfor1minandrehydratedinNaCl/Pi. C ells were incubated w ith the primary antibody for 60 min at room temperature, washed three times withNaCl/Pi, incubated with the secondary antibody for ... this way. For analysis o fthe s teady-state distribution of GFP–MRP2 proteins, cellswere induced with 5 mMbutyrate for 24 h [16] and observed48 h a fter start o f transfection. For a nalysis...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... goodmodel for performing structural studies. In the presentstudy, we therefore used SDS to structurally character-ize the oligomerization process of C-peptide and ana-lyzed the formation of ... whereas formamide breaks hydrophobicones. The addition of 50–150 mm NaCl reduced theoligomer formation of C-peptide (Fig. 1D) and form-amide had no effect (Fig. 1D). The combined resultstherefore ... aggregates are predominantly inb-sheet conformation, and that the oligomerization process shows charac-teristic features of amyloid formation. The formation of large, insoluble,b-sheet amyloid-like...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... reductase (PET-NR) from Enterobacter cloacae. For reference, inTable 1, we summarize the flavoproteins for which aspecific H-tunnelling study has been performed. Gener-ally, the KIEs of H transfer ... NAD(P)H binding and dissocia-tion can be measured by following the formation of thisCT absorbance at, for example, 555 nm while perform-ing a concentration dependence [35,39]:kobs¼ koffþ kon½NADðPÞHð6ÞSimilarly, ... availablefrom Melford Laboratories (Chelsworth, UK). We useextinction coefficients of 6.22 mm)1Æcm)1at 340 nm for NAD(P)H isotopologues and 16.8 mm)1Æcm)1at 289nm for NAD(P)H4[34]....

Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

... long-term function-oriented structural effort aimed at understanding the molecular bases ofpolyQ disease proteins, we report here a study of the structural determinants for the interactions of Anp32awith ... London) for providing the Lanp clone,which was produced from a cDNA originally providedby Dr A. Matilla (ICH, London), Filippo Prischi for help with the gromacs software, and Dr L Masino for helpful ... Journal compilation ª 2008 FEBS Structural bases for recognition of Anp32⁄ LANP proteinsCesira de Chiara, Rajesh P. Menon and Annalisa PastoreNational Institute for Medical Research, The Ridgeway,...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... cleft formed by the twodomains. A common functional conformational change has been reportedin this protein family, which involves switching from an open formto a sugar-transporter bindable form, ... designated a closed form. TheTvuCMBP–c-cyclodextrin complex structurally resembles the closed formof EcoMBP, indicating that TvuCMBP complexed with c-cyclodextrinadopts the closed form. The fluorescence ... defined in terms not of the affinities for sugars but of whether the protein adopts the openform or the closed form. TvuCMBP–c-CD complex isseen as the closed form (Fig. 2A,B), and this struc-ture...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... deconvolu-tion program for each p26 variant generated by site-directedmutagenesis and for purified wild-type (WT) p26 from transformedEscherichia coli and Artemia embryos. Structural elementsR110G(%)F112R(%)R114A(%)Y116D(%)WT(E. ... Sequencing Facility, Center for Applied Genomics, Hospital for Sick Children,Toronto, ON, Canada).Bacterial synthesis and purification of p26p26 was synthesized in transformed E. coli BL21PRO(Clontech ... col-onies were counted after incubation for 24 h at 37 °C. Allexperiments were performed in triplicate. Between datagroups, two-sample t-tests were performed at a confidencelevel of 95% with...

Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx

... transcriptase (Promega) at 37 °C for 1 h in the appropriate buffer. The reaction was stopped byincubation at 70 °C for 10 min. iQTMSYBR Green supermixPCR kit (Bio-Rad) was used for real time monitoring ... well), incubated at 28 °C for up to 24 h and then exam-ined by microscopy for phenotype analysis. Unfertilizedeggs were removed after 4 h. Alternatively, embryos wereﬁxed for in situ hybridization ... clarify whetherthis receptor is active or not in its full length form.Expression of both the wild type and dominant negat-ive forms of Cg-TGFbsfR2 give rise to the same phe-notype and modiﬁed...

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... Biochem 150, 76–85.T. Meier et al. Structural evidence for a constant c11ring stoichiometryFEBS Journal 272 (2005) 5474–5483 ª 2005 FEBS 5483 Structural evidence for a constant c11ring stoichiometryin ... feature for function [14]. The number ofbinding sites on the c ring determines the ATP toproton ⁄ Na+ratio, and therefore this stoichiometry isan important bioenergetic parameter for the ... (–) and the c ring incubated at 95 °C for 5 min. The gel was stained with silver. A molecular mass stand-ard is shown.T. Meier et al. Structural evidence for a constant c11ring stoichiometryFEBS...

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