Báo cáo khoa học: RNAi-mediated knockdown of juvenile hormone acid O-methyltransferase gene causes precocious metamorphosis in the red flour beetle Tribolium castaneum pdf
Báo cáo khoa học: RNAi-mediated knockdown of juvenile hormone acid O-methyltransferase gene causes precocious metamorphosis in the red flour beetle Tribolium castaneum pdf
... 2008 The Authors Journal compilation ª 2008 FEBS 2931 RNAi-mediated knockdown of juvenile hormone acid O-methyltransferase gene causes precocious metamorphosis in the red flour beetle Tribolium castaneum Chieka ... positions of the introns are indicated by red lines, and the numbers above indicate the positions of the introns asdescribed in the text.C. Minakuchi et al. Function of JHAMT gene in Tribolium FEBS ... expressedspecifically in CA until the beginning of the final larvalinstar [9]. In contrast, the BmJHAMT transcript isundetectable in CA at the beginning of the spinningstage, but is detected at low levels in the...
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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf
... Similarly, the 33 orderedresidues in the T structure (that are disordered in the N form) are not related to the Tris-binding site resi-dues in any way. Thus, the effect of Tris binding on the glutaminase ... aa; nine aa in total) were locatednear the interface of the physiological dimer. The C-terminal domain may contain structural featuresinvolved in protein–protein interactions to form the physiological ... report the crystal structure of the intact glu-taminase under four different conditions: in the absence of the additives (referred to as N); in the presence of Tris (referred to as T); in the presence...
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Báo cáo khoa học: Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir-binding site potx
... of 3.8 A˚ .In PRV82A, the change in the position of the main chainatoms placed the Cb atom of Ala82 within reasonable vander Waals distance of indinavir (4.1 A˚), resulting in a loss of ... oxygen of Gly27, the OD1 of Asp29¢ and the NE atom of Arg8. The N4 atom of indinavir interacted with the carbonyl oxygen of Gly27¢. The indinavir O1 and O3 atoms formedhydrogen bond interactions ... change in position of the pyridyl end in PRL90M(Fig. 4A). The pyridylgroup of indinavir is accessible to the surface, while all the other groups in indinavir are shielded either by a network of water...
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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... demonstrating that the binding of Glc at the active site indeed induces a large-scale domain move-ment that closes the active site cleft and creates the stereochemical environment for binding of the ... involved in the binding of D-glucose in the super-open state (PDB identity: 1v4t). The structural imageswere generated usingPYMOL, version 0.99.Activation of glucokinase byD-glucose binding ... amino acids in the L-domain (residuesN204, D205, N231, E256 and E290) and the S-domain(residues T168 and K169) (Fig. 1B). To identify the contact residues involved in the initial binding of...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... RNA-bindingand enoyl-CoA-hydratase activity. In addition, it was shown that muta-tions in the AUH gene are linked to MGA1. Here we present kinetic data of the purified gene product of AUH using ... hydratase’). The RNA-binding activity of the human protein and also of the murine homologue was investigated further, its biologi-cal function, however, remained unclear [16,17]. The three-dimensional ... from human brain cells by affinity chro-matography using the immobilized RNA-oligonucleo-tide (AUUUA)5or ‘AU’ followed by cloning of the corresponding gene [15]. Interestingly, the gene showedsequence...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
... weredetermined (Table 1). The binding affinities of the inhibitorto both binding sites, yielding the IE or EI complex, wereonly slightly different. On the other hand, the bindingaffinity of the second ... Ile8 of the peptide interacting with the Tyr752 hydroxyl group of the enzyme. A hydrophobicinteractionofthesidechainofMet1ofTat(1–9)withthephenyl ring of the substrate Ala-Pro-pNA resulted in ... Asp739 of DP IV resulting in a considerable stabilization of the complex. The interactions of the C-terminus as well as the N-terminus of Tat(1–9)-related peptides permitted the dock-ing of the inhibitor...
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Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt
... c552proteins,respectively. The band denoted by the asterisk on the right-handside is the result of nonspecific staining of the extracts containing the PH c552protein.Biogenesis of cytochrome c¢ H. Inoue et ... results that the co-expression of the ccm genes in the pEC86 plasmid and the presence of the DsbD protein are not necessarily required for the heterologous synthesis of the PHCP protein byE. coli, ... 50 mL of the same buffer containing 0, 0.2or 1.0 m NaCl, a red- colored fraction containing the PHCPprotein being eluted with 0.2 m NaCl. The red- coloredfraction was further dialyzed against...
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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot
... by the main fuelsources in muscle and by insulin through directlyaltering the expression of the human PDK2 and PDK4isoforms. In addition to our findings, insulin has been shownto decrease the ... expression of these isoforms. The regulatory in uence of glucose, fatty acidsand insulin on PDK2 and PDK4 mRNA expressionWe examined the effects of the two predominant meta-bolic fuels in muscle, namely ... 2A,B).Downstream targets of PtdIns3K include glycogen syn-thase kinase-3 (GSK3) and the mammalian target of rapamycin (mTOR). GSK3 is inactivated in responseto insulin via a PtdIns3K ⁄ protein kinase B (PKB)-dependent...
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Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deficiency in the inner ear of rats with D-galactose-induced aging pdf
... 5.1-fold,respectively.Increased protein level of TFAM induced byD-GalTo further understand the expression of TFAM protein in the inner ear, western blot analysis was performed. The protein levels of TFAM in the ... in mutant mtDNA harboring the com-mon deletion. By applying the specific probe, we caneven detect the presence of the common deletion in the inner ear in the control group. The proportions of ... explore the possible mechanisms involved in the development of presbycusis. In the present work, we demonstrated significantlyincreased levels of the mitochondrial common deletion in the inner ear of...
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Báo cáo khoa học: Intracellular degradation of somatostatin-14 following somatostatin-receptor 3-mediated endocytosis in rat insulinoma cells doc
... min. The recov-ery of surface binding sites was determined as describedabove. Interestingly, during the first 15 min of incuba-tion, surface binding recovered to 76%. However, incu-bation of ... subtypes in their inter-action with arrestins indicate the existence of internali-zation and trafficking pathways that are specific for the SSTR subtypes.Determining the fate of the internalized ... Stimulation of SSTR2Awith octreotide induced long-lasting sequestration of the intact ligand into early endosomes.Here, we investigated the intracellular trafficking of SSTR3 and the processing of internalized...
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Từ khóa: Nghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roBT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam