Báo cáo khoa học: Hatching enzyme of the ovoviviparous black rockﬁsh Sebastes schlegelii – environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene docx

... FEBS Hatching enzyme of the ovoviviparous black rockfish Sebastes schlegelii – environmental adaptation of the hatching enzyme and evolutionary aspects of formation of the pseudogene Mari Kawaguchi1, Masahiro ... University of Tokyo, JapanAt the time of hatching of oviparous fish embryos, the hatching enzyme is secreted from hatching gland cells of the embryos to digest the egg envelope (chorion) [ 1– 3]. The hatching ... envelope (chorion) at the time of embryo hatching. In the present study, we investigated the hatching of embryos of the ovoviviparous black rockfish Sebastes schlegelii. The chorion-swelling activity,...

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... bombyxin and insulin,which are of the order of 100 pm [23,24]. The veryhigh titer of 8K-BLP may be due to a remarkably highlevel of gene expression in the fat body and to the large volume of this ... instead of as aheterodimer. Second, 8K-BLP was predominantly pro-duced by the fat body, a functional equivalent of the liver and adipose tissue of vertebrates, and the liver is the major source of ... Protein contents in the female (C) and male (D) disks before and after cultivation for 5 days. Values are the means and standard errors of the mean (n = 6). (E–H) Confocal images of tissues with...

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... browning of fruits and vegetables, and morphogenesis and fruiting body formation in fungi.All of these processes involve melanin formation. In the bacterial kingdom there are some examples of well-characterized ... be part of the defense sys-tem against wounding and infection by the pathogen[16]. Thus, the timing and location of melanizationseems to be essential for the prevalence of one of thesetwo ... in the PPO genesRSc0337 and RSc1501. TH activity was determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS.Fig. 3. Dependence of the TH activity of extracts of R3 and the two...

Tài liệu Báo cáo khoa học: "Discovering Global Patterns in Linguistic Networks through Spectral Analysis: A Case Study of the Consonant Inventories" pdf

... example, the prin-cipal eigenvector (i.e., the eigenvector correspond-ing to the largest eigenvalue) is the direction inwhich the sum of the square of the projections of the row vectors of the ... we are aware of that analyzes the eigenvectors to obtain a two dimensional visualize of the network. Nevertheless, the work does notstudy the spectrum of the graph. The aim of the present work ... threetaken together. The individual contribution of the other eigenvalues to the spectrum is signiﬁcantlylower than that of the top three. Third, the eigen-values on either ends of the spectrum...

Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx

... pharmacological,inﬂammatory and toxic effects with those of the parentmolecule MDP. The results reported establish the safety of MB, the absence of undesirable effects on the central nervous system, and the lack of ... by the Institutional Animal Care and Use Com-mittee of the University of Balamand and complied with the principles of laboratory animal care.Chemicals and reagentsMuramyl peptides (MDP and ... elec-trons through the respiratory chain, increasing the time of interaction between these electrons and molecularoxygen and facilitating the formation of ROS.Activation of innate immune cells...

Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus inﬂuenzae Identiﬁcation of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx

... H-7BHepI fi3,4)-L-a-D–Hepp-(1fi 5.0 5–5 .18a4.0 0–4 .06a4.0 1–4 .04a4.25b – c4.13b – 97. 4–9 8.8a71. 1–7 1.2a73.8 74.3 – 68.5 – HepII fi2)-L-a-D–Hepp-(1fi 5.6 2–5 .73a4.18 3.94 3.96 3.77 ... to the partial occurrence of PEtn attached to the phosphate groupat O-4 of Kdo [11,12,15].Structure of the core region of the Hep3-glycoforms. In the 1H NMR spectra of OS-1¢-A (Fig. 5A) and ... (loss of t-Hex), 739.3 (loss of t-Hex–Hep) and 693.3 (corresponding to t-Hex–Hep-Hex),which showed the trisaccharide element Hex–Hep-Hex tobe attached to HepI. The topology of the other glycoformswere...

Báo cáo khoa học: A chloroplast RNA binding protein from stromal thylakoid membranes speciﬁcally binds to the 5¢ untranslated region of the psbA mRNA potx

... 22fractions of 500 lL; 10 lL of these fractions were used forUV cross-linking experiments.In vitrosynthesis of RNA and UV cross-linking of RNAwith proteinsTemplates for the in vitro synthesis of ... reported on a set of chloroplastRBPs, which interact with the 5¢ UTR of the psbD mRNAencoding the D2 protein of PS II of C. reinhardtii. Amongstthose, a protein of 63 kDa (RBP63) cofractionated ... involved in the light and/ or redox regulation of D1 synthesis. RB47 was localized to the LDM system [9] and, in addition, RB60 was shown to be partitionedbetween the stroma and the membrane...

Báo cáo khoa học: "TENSE GENERATION IN AN INTELLIGENT TUTOR FOR FOREIGN LANGUAGE TEACHING: SOME ISSUES IN THE DESIGN OF THE VERB EXPERT" pot

... identify the errors, if any, present in the former and to formulate some hypotheses about their causes. On the basis of these hypotheses, the Tutor selects the next exercise which will test the ... example, the kind of clause (main, coordinate, subordinate), whether the clause has a verb to be solved, the voice and form of the clause, the kind of event described by the clause, the time ... illustrates the ET general architecture and mode of operation. Section four constitutes the core of the paper and presents the design re- quirements, knowledge bases and reasoning algorithms of the...

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... Examination of the kinetics of the reaction revealed a signiﬁcantlyhigher afﬁnity of the soybean synthetase for canavanine(69 lm) compared with that of the jack bean enzyme (1.3 mm), and the corresponding ... isachieved by the E. coli enzyme, the soybean enzyme reveals a discriminatory ability that has characteristicsapproaching those of the jack bean enzyme. In view of the distinct role of conformational ... comparison of amino acid utilization by the plant arginyl-tRNA synthetases. The aminoacylation level attained in the pres-ence of L-arginine was compared to that in the presence of 1 mM of the analogue...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

... in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERbwas increased in both ERE1 and ERE2 of the HOXC13 promoter (Fig. 5A, lanes 1–4 ). The levels of E2-induced ... with the control, HOXC13 expression wasfour-fold to ﬁve-fold higher in the presence of 100 and –2 288 –2 152 –2 000 –1 788 –1 260 –3 000 – 234ERE6ERE5ERE4ERE3ERE2ERE1+1 – 234ERE6ERE5ERE4ERE3ERE2ERE1+1Fig. ... Interestingly, the kinetics of E2-dependent binding of ERa and ERb to both ERE1 and ERE2 are differ-ent. The binding of ERa is very low in the absence of E2, and is signiﬁcantly enhanced in the presence of...

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