... Sulfide : quinone oxidoreductase (SQR) from the
lugworm Arenicola marina shows cyanide- and
thioredoxin-dependent activity
Ursula Theissen and William Martin
Institute ... A. marina can use elec-
trons from sulfide for mitochondrial ATP production. In bacteria, electron
transfer from sulfide to quinone is catalyzed by the membrane-bound flavo-
pro...
... reference, the
position of the sugar and of the metal in the crystallo-
graphic template 1YOE. The two ligands were modelled in
each binding site of SsCU-NH, and then the structure of
the resulting ... and
FUGUE [60] and gave the same results, therefore confirm-
ing the correctness of the chosen templates. The sequences
of target and templates were aligned to ea...
... terms from the corpus by using Naka-
gawa’s method. These extracted terms be-
come the candidates for the final step. The
final step, filtering step, removes inappro-
priate terms from the candidates ... explanation of the term.
4. There are several technical terms that are re-
lated to the term.
We have implemented the checking program of the
first two conditions in the s...
... MA, USA), and the band of
interest was excised from the 1% agarose gel. DNA was
extracted from the gel with the QIAquick Gel Extraction kit
(Qiagen, Hilden, Germany), and cloned with the Zero ... calculated from the obtained measurements,
the background fluorescence was subtracted, and the
resulting value was compared with that of the AtxC
sample. The influence o...
... present in large quantities in the
extract, could be favored. On the other hand, the
coincidence between the N-terminal sequences of
SmCP and those from several other MCPs included in
alignments ... presence of other domains in
addition to the CP domain [2,3]. On the other hand,
SmCP shows sequence homology with some CPs iso-
lated from different vertebrates and inverteb...
... b-glucosidase, the subunit
interfaces involve fewer electrostatic interactions such
as salt bridges and ion pairs than in the case of the
enzymes from the hyperthermophiles S. solfataricus
and Thermosphaera ... clear,
as there was a difference in activity after 10 and
70 min. Here, the enzyme was still inactivated in time.
Figure 1 also shows the extent of the inacti...
... (8 8:5 : 2:5 ,
v ⁄ v), 5 mLÆ0.2Æg
)1
of resin at room temperature for 2 h.
After filtration of the exhausted resin, the solvent was con-
centrated in vacuo and the residue triturated with ether.
The ... donation and all were
non-smokers. The study was approved by the local Ethics
Committee, and informed consent was obtained from all
participants.
Random locomotion an...
... problems and follows the
same basic folding rules in the cytosol and ER. The
chaperones that assist the nascent chains in these two
compartments are related: members of the Hsp70 fam-
ily and their ... circumstances.
The dynamic nature of complexes in the ER, the
continuous dynamic restructuring of the organelle as a
whole, and the presence of sensors that detect w...
... could be directed at the donor, the harvested organ
ex vivo or the recipient and would clearly need to be
efficacious, be specific, lack side-effects and be easily
deliverable to the organ in question. ... inhibition and Nrf2 gene silencing. The
fold-increase in both HO-1 protein and mRNA in
response to insulin was consistent, as well as being
time and concentration dependen...
... ligands: the three histidines and the first gluta-
mate ⁄ glutamine of the motif. When using multiple
clustalx [9] alignments of the MCA0715-encoded
protein with members of the Hr (Fig. 4A) and ... pointing towards the inside
of the bundle, and Cys128 was located to the random
coil of the C-terminal end of the protein (C-terminal
to helix IV) (not shown). The long d...