Báo cáo khoa học: Molecular characterization of gonad-inhibiting hormone of Penaeus monodon and elucidation of its inhibitory role in vitellogenin expression by RNA interference pptx

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... mechanism of RNA control in animals, the function of a diversenumber of RNA- binding proteins has been elucidated[1–4]. Despite this, translational control through thebinding of RNA- binding proteins ... poorly described in plants.PUF proteins are a large family of RNA- bindingproteins found in all eukaryotes. These proteins reducethe expression of mRNA targets by binding in 3¢ UTRregulatory ... repressing its translation and promoting germline stem cell development [12–14].C. elegans FBF also controls germline stem cell main-tenance by regulating gld-1 mRNA expression and sus-taining...

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... because in terms of func-tion, the fish intestine is highly regionalized both in thelarva and in the adult [38]. The posterior segment of the fish intestine is the absorption site of intact pro-teins, ... lining the digestive tract lumen[43,44]. Considering the importance of the intestinalbarrier in the process of oral prion infection, this find-ing might help clarify the entry and routing of ... residuesinstead of 15 residues, including strand S2, betweenhelices H1 and H2, and 84 residues instead of 10 resi-dues between helices H2 and H3 (Fig. 2). Molecular characterization of zebrafish...

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... allergen-induced histamine release was calculated as percent of thetotal amount of histamine determined after lysis of thebasophils by twofold freezing and thawing of the cells. Ahistamine release ... participating in an IgE-bindingepitope. The N-terminal sequencing of a 52-kDa glyco-protein of tomato extract gave hints for the existence of b-fructofuranosidase as a relevant allergen in tomato[15,16]. ... no inhibition atall (Fig. 5A). Binding to the contaminating 90-kDa bandwas also inhibited by MUXF, so this protein may be anIgE reactive glycoprotein as well. Preincubation of a non-CCD binding...

Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

... cassette, resulting in a Scpep1 ⁄ b-galactosidase ⁄ neo-mycin phosphotransferase fusion protein. The fusion protein is devoid of the C-terminal half of Scpep1, including two amino acids of the assumedcatalytic ... 1. Molecular forms of Scpep1. (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and ... (Pharmacia) and eluted in 20 lL fractions at a ﬂow rate of 40 lLÆmin)1, which were analysed by western blot using the rabbit a-Scpep1 antibody. A mixture of molecular mass standard proteins, including...

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... structures. At 10 min,punctate staining diminished and vacuolar staining in- creased, and ﬁnally punctate staining was almost lostwith predominant vacuolar staining at 30 min. Com-pared with ... of Medicine, National University of Singapore, Republic of Singapore2 Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republic of Singapore3 Institute ... phosphatidylinositolphosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,PtdIns(3,4)P2 and PtdIns(3,5)P2with the strongestbinding to PtdIns(5)P (Fig. 1). The ORD domainshowed a very similar lipid binding pattern as...

Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... thequantification of microgram quantities of protein utilizing theprinciple of protein-dye binding. Anal. Biochem. 72, 248–254.19. Weiss, R. (1970) The solubility of nitrogen, oxygen and neon in seawater. ... Sprinzl, M. (1992) Molecular cloning and nucleotide sequence of the gene encoding aH2O2-forming NADH oxidase from the extreme thermophilicThermus thermophilus HB8 and its expression in ... Also, in thepresence of FMN instead of FAD, or NADPH instead of NADH no NoxC activity was found For this reason,further purification and characterization of NoxC wasabandoned.Purification of the...

Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... : 26 of the original volume using Centricon-10 (Amicon).Proteins were determined by the modiﬁed method of alkaline copper (Lowry) protein assay [23] using BSA as thestandard.Transglutaminase ... incorporation of monodansylcadaverine into N,N-dimethylc asein with Km and Vmaxvalues of 0.35 mM and 13.3 n molÆmin)1Æmg)1,respectively, indicating that the predicted protein is atransglutaminase.Subcellular ... ScientiﬁcResearch from the Ministry of Education, Science, Sports and Culture,Japan, and by Special Coordination Funds for Promoting Science and Technology of t he Science and Technology Agency of the JapaneseGovernment.REFERENCES1....

Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... cascade involving multiple proteases. In endoparasites, aspartic, cysteine and metalloproteas-es are found in the intestine, and the analysis of Hbproteolysis by those recombinant proteases indicatesthat ... staining kit (Dai-ichi-Pure Chemical, Tokyo, Japan). The densities of stainedbands were measured using image j 1.36b (National Insti-tutes of Health) and the ratio of the density divided by ... gambiae: molecular cloning and biochemical characterization of two inducible chymo-trypsins. Eur J Biochem 268, 4027–4035.33 Akov S (1982) Blood digestion in ticks. In Physiology of Ticks (Obenchain...

Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... tivities and s omeproperties of 5¢-nucleotidase, adenosine kinase and adenosinedeaminase in tissues of vertebrates and invertebrates in relation tothe control of the concentration and the physiological ... screenwas by n o means exhaustive, and although t he proteinkinases tested in this study represent most of the p rincipalclasses of protein serine/threonine kinases, the possibilityremains that ... of the deduced aminoacid sequence of mAK-S. In order to study the function and regulation of mouseAK in vitro, both isoforms were s ubcloned into a pET expression vector encoding an N-terminal...

Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx

... unfolding of the proteins is required for dissociation of the complex and membrane binding. In the presence of membranemimetics, the a-helical contents of Rv3619c and Rv3620 increased by 42% and ... respectively. In mice, the immune response against Rv3619c pro-tein is characterized by increased levels of interferon-c, interleukin-12 and IgG2a, indicating a dominant Th1 response, which is mandatory ... Rv3020ccontains histidine in place of tryptophan in the WXGmotif, which induces the formation of a small helixthat joins the N-terminal and C-terminal domains [13].The ESAT-6 ⁄ CFP-10 homologs in Staphylococcusaureus...

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