... ¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent
cationic lipids for gene delivery – synthesis, in vitro
transfection activity, and physicochemical characterization
Michael Spelios and Michalakis Savva
Division of Pharmaceutical ... PL
(1994) Enhanced gene delivery and mechanism studies
with a novel series of cationic lipid formulations. J Biol
Chem 269
,...
... ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and
related metabolic diseases, and discuss the possibility that both could func-
tion as molecular targets for the prevention and treatment of obesity and
metabolic ... focus on
the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes-
ity and related metabolic diseases, and discuss whether
these ANGPTLs could be targets for the prevention
and...
... The K
m
value for NADH was determined in the pre-
sence of FNR (200 n
M) and NADH (0. 5–7 .0 mM), and the K
m
value
for NADPH was determined in the presence of FNR (20 n
M) and
NADPH ( 2–1 00 l
M).
NADH ... vector, and the
construct was designated pET–FNR. E. coli BL21(DE3)
Codon Plus cells were transformed with pET–FNR. The
transformant was grown in 2 · YT medium containing
c...
... template for a second cycle of mutagenesis
and selection (10 000 mutants). It afforded 351 novel
candidate strains. After rescreening, 10 mutant proteins
were purified and characterized, and their ... were
supplied by Fluka (Buchs, Switzerland). LB (Luria–Bertani)
medium was from Becton Dickinson (Basel, Switzerland).
Generation of mutant libraries
For the generation of the ribA muta...
... attributed to mutations in
helicase genes [ 1–4 ]. Helicases act on double-stranded
nucleic acid substrate and thus can be designated
DNA–DNA, RNA–DNA or RNA–RNA helicases
depending on the composition ... been
suggested for a number of the conserved motifs [1 0–
12]. For example, motif I (A ⁄ GxxGxGKT), motif II
(VLDEAD), motif III (SAT) and motif VI
(HRIGRxxR) are responsible f...
... 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former,
and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢
and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCA
GGGG-3¢ for the latter. The PCR products were cloned into
the EcoRV site of pZErO-2 (Invitrogen). ... 200 lm. (B) Prox–1 and VEGFR–3 were also expressed in
Lyve–1-positive ECs, confirming that they maintained lymphatic
endothelial properties. (C) VEGF–A and VEG...
... bonded pairs have different C
a
and
C
b
distances than hydrogen bonded pairs and therefore may
have different residue preferences and potential for side
chain–side chain interactions. Initial ... varied (for instance, core
residues 3 and 39, and boundary residues 25 and 29 that
were all changed in a hyperstable GB1 variant [12]). Based
on the computational studies, core positio...
... Association for Computational Linguistics, pages 38 3–3 87,
Jeju, Republic of Korea, 8-14 July 2012.
c
2012 Association for Computational Linguistics
Unsupervized Word Segmentation:
the case for Mandarin ... II (Emerson, 2005)
and define a more precise topline for the task
using cross-trained supervized system available
off-the-shelf (Zhang and Clark, 2010; Zhao and
Kit, 2008;...
... 77 1–7 77.
Stem–loop padlock oligonucleotides for dsDNA B. Ge
´
ron-Landre et al.
5352 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEBS
Stem–loop oligonucleotides as tools for labelling
double-stranded ... TÆAxT and CÆGxG triplets with an antiparallel
TG TFO.
Stem–loop padlock oligonucleotides for dsDNA B. Ge
´
ron-Landre et al.
5344 FEBS Journal 272 (2005) 534 3–5 352 ª 2005 FEB...
... the
in-domain gold standard classification result. For
example, the in-domain goal standard for the Book
domain is 79.96%. For adapting from DVD to Book,
baseline achieves 72.25% and JST gives 76.45%.
The ... 257859).
References
R.K. Ando and T. Zhang. 2005. A framework for learn-
ing predictive structures from multiple tasks and un-
labeled data. The Journal of Machine Learning...