Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC

Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot

... 5¢-AAGGTGCAGAAGACGCA CTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢; cdk4-F, 5¢-TACAACAGCACCGTGGACAT-3¢; cdk4-R, 5¢-TGGGCATCGAGACTATAGGG-3¢; rp49-F, 5¢-CGG ATCGATATGCTAAGCTG-3¢; and rp49-R, 5¢-GAACG CAGGCGACCGTTGGGG-3¢. Acknowledgements We ... Takeuchi T, Yamazaki Y, Katoh-Fukui Y, Tsuchiya R, Kondo S, Motoyama J & Higashinakagawa T (1995) Gene trap capture of a novel mouse gene, jumonj...

Ngày tải lên : 23/03/2014, 07:20

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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

... 5¢-GGTGGTA GATGATCGGCAAGGTTTGC-3¢), C130S (forward 5¢- CCAATTGGTGTGCTAAAGACTTTG-3¢/reverse 5¢-AA GGATAAATATGTGAGAAGATATTC-3¢), C133 (forward 5¢-CTTTGAAAAATATATCAGAGAAAATG-3¢/ reverse 5¢-TCTTTAGCAGACCAGTTGTAAGG-3¢), C159S ... (forward 5¢-GCCCACAATAAAGTCAATAAG AAAT-3¢/reverse 5¢-CTCAGACATCCACCTCCCAAG TTCTT-3¢),C176S(forward5¢-CTCCAATTTCTGG GAAAAAAGATGGAAG- 3¢/reverse 5¢-TCAAATTTGG GCTTCCTCAA...

Ngày tải lên : 17/03/2014, 10:20

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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... reactions. The first round of PCR reaction was performed with a forward primer UPM (a mixture of primers 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAAT ACGACTCACTATAGGGC-3¢) and ... products then were used as the templates for the second round of PCR reaction. The fragment named CaLP2 was amplified with a nested forward specific primer LS12 (5¢-CACAGACGGCAAT...

Ngày tải lên : 16/03/2014, 23:20

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Tài liệu Báo cáo khoa học: The alr2505 (osiS) gene from Anabaena sp. strain PCC7120 encodes a cysteine desulfurase induced by oxidative stress docx

... (5¢-to3¢) rnpB Forward: AGG GAG AGA GTA GGC GTT GC Reverse: GGT TTA CCG AGC CAG TAC CTC T isiA Forward: GCC CGC TTC GCC AAT CTC TC Reverse: CCT GAG TTG TTG CGT CGT TA alr2495 Forward: AAA ACG GCT GCA GTT ... in Anabaena remains unanswered. On the basis of the data obtained for the Anabaena genome presented in Fig. 4, it is tempting to speculate that the SUF system [alr2492(SufB)...

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... they can increase the amount of available phosphate by interacting together. Additionally, fusing PhyH-DI to a single-domain phytase appears to be an efﬁcient way to improve the activity of the ... phytases (his- tidine acid phosphatase (HAP), cysteine phytase and purple acid phosphatase) by having a neutral (pH 7.0) rather than acidic pH optimum. Previous studies have shown that...

Ngày tải lên : 14/02/2014, 15:20

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Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... amidohydrolase that participates in picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzyme stands at a branch point of the tryptophan to NAD pathway, and deter- mines the ﬁnal ... solution [18]. Therefore, the available structural data suggest that the minimal functional unit in the ACMSD enzyme is a monomer. and the biological signiﬁcance, i...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation docx

... Arabidopsis A 4 -GAPDH The sequence encoding the putative mature form of the A. thaliana plastidial A 4 -GAPDH isoform (GapA-1 cDNA At3g26650 provided by TAIR, Stanford, CA, USA) was ampliﬁed by PCR using the ... the molecular mass of A 4 -GAPDH shifted back to the mass of the untreated protein. These results clearly demonstrate that Arabidopsis A 4 -GAPDH can underg...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: "The Eﬀect of Corpus Size in Combining Supervised and Unsupervised Training for Disambiguation" pdf

... over all MI values of the lattice as a measure of the a nity of attachment phrase and attachment node. The intuition is that we are looking for the strongest evidence available for the attachment. ... mutual informa- tion values for Example 1. The highest values are 12.2 for high attachment (mechanism) and 3 for low attachment (System). The algorithm therefore...

Ngày tải lên : 20/02/2014, 12:20

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Báo cáo khoa học: The hinge region operates as a stability switch in cGMP-dependent protein kinase Ia doc

... illustrated in Fig. 5A, B. Overall, it was observed that the D1-77 degradation was much slower, indicating that the formation of PKG D1-201 from PKG D1-77 is slower than the cleavage at R77. Formation ... indicating that a confor- mational change has occurred that increases the solvent exposure of this region [22]. Crystal structures of a similar enzyme from the AGC-fa...

Ngày tải lên : 07/03/2014, 09:20

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Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf

... spectra identical to that of the WT protein. Only these vari- ants of GM2AP were examined in all further studies. To assess the potential of the variant GM2AP forms for the stimulation of HexA catalysed ... C112 forms a ‘clamp’ that structurally restrains the activator’s helical domain. It is therefore most likely that the mutation C138R distorts the orien- tation o...

Ngày tải lên : 07/03/2014, 12:20

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