... organization of the human AbH-J-J
locus and identification of the transcription
initiation sites proximal to the P1 promoter
The structural organization of the human AbH-J-J locus
is shown in Fig. 1. The ... attention on the activity
of binding of nuclear extract to these boxes.
Binding of nuclear factors to GC-rich boxes
of the Ab...
... sero-
type E and L
2
were of similar activity and both less active
than smooth LPS by a factor of 100 whereas, LPS from
Chl. psittaci was of lower activity than the two other
chlamydial LPS. Nevertheless, ... ratio of 5 : 5 : 0.01 (v/v/v) and
sprayed at a flow rate of 2 lLÆmin
)1
.
MALDI-TOF MS of LPS and free lipid A were
performed on a Reflex III (Bruker-Daltonik,...
... added to the cotton meal,
respectively (not shown). The time dependency of the
entomotoxic effect was similar for both proteins, with a lag-
phase of 3–4 days for death of the first insects, and reaching
maximal ... characterize and to study the mode
of action of entomotoxic ureases in order to establish their
biotechnological potential against phytophagous insect...
... subunit, and one of a
variety of r egulatory B subunits. These diverse B subunits in
the P P2A heterotrimer allow the phosphatase to localize t o
distinct regions of the cell and to dephosphorylate speci®c
substrates, ... level of nonspeci®c adsorption of the
B56a polypeptides to the beads, and suboptimal binding in
the absence of cotranslation of the...
... Snail and SP- 1, but not the association of Snail
with SP- 1.
Chromatin immunoprecipitaion assay for in vivo
DNA -binding activity of the candidate
transcriptional factors
To further examine whether ... transcriptional factors induced by TPA.
Taken together, by examining the pattern of SII we
suggest that the in vitro DNA -binding activity of all
three transcr...
... with the
higher inhibitory activity of the cEL peptide and the very
low inhibitory activity of the cYT peptide compared to
other peptides.
The flanking residue of the b-turn, i.e. Tyr3 of the cVY
peptide ... suggestive of a b-turn in the peptide and
the proximity of amide protons due to compact nature of
the structure. However, t he coupling consta...
... between the two isoforms of the protein,
only the molecular mass of 50 kDa would suggest that we
had purified the truncated isoform.
Cloning of the cDNA of two isoforms of tomato
b-fructofuranosidase ... b-fructofuranosidase of tomato was
further confirmed by Foetisch et al. [22]. The aim of the
present study was to analyze the role of N-linked glycans in
the...
... any reactivity with the Toll-like receptors TLR-
2 and -4, in contrast to control lipopeptides and LPS. These
data confirm the applicability of our conformational concept
of endotoxicity to nonlipid ... assay
The LAL test is based on the property of lysates of
amebocytes of the horseshoe crab Limulus polyphemus ,to
form a solid gel in the presence of minute amo...
... affect the precise positioning of
the aromatic ring of Tyr456 in the closed state. In the
absence of a closed complex of E542K P2Ox, the effect
of the loss of this hydrogen bond on the packing of
the ... from the internal
cavity of the homotetramer. Concomitantly, Phe454
rotates and moves some 7 A
˚
to fill the active site and
pack against the FAD...
... alter the routing of the
receptor to the cell surface, resulting in loss of basal activity
and loss of agonist induced cAMP production [14–16].
In order to study the mechanism of activation of the
TSHr ... corresponds to the major unliganded form of the
receptor; the active conformation capable of promoting
GDP/GTP exchange on the G-protein is achieve...