Báo cáo khoa học: Identiﬁcation of an osteopontin-like protein in ﬁsh associated with mineral formation pot

Báo cáo khoa học: Identiﬁcation of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockﬁsh Sebastes schlegeli pot

... and a mature protein starting with Ile59, in accord with the result of N-terminal protein sequence analysis. It is likely that the mature protein iscomposed of 496 amino acids with a calculatedmolecular ... 2006)doi:10.1111/j.1742-4658.2006.05570.xFish skin mucus contains a variety of antimicrobial proteins and peptidesthat seem to play a role in self defense. We previously reported an antibac-terial protein in the skin secretion of the ... & Yonehara S (2000) Puriﬁcationand cloning of an apoptosis-inducing protein derivedfrom ﬁsh infected with Anisakis simplex: a causativenematode of human anisakiasis. J Immunol 165,1491–1497.25...

Báo cáo khoa học: Identiﬁcation of an osteopontin-like protein in ﬁsh associated with mineral formation pot

... Osteopontin and osteopontin-like gene and protein struc-ture. (A) Structural organization of osteopontin-like (tetraodon andzebrafish) and osteopontin (chicken, bovine, mouse and human)coding sequences ... OP-L protein is indeed the fish equivalent of mammalian osteopontin.OP-L protein plays a role in the process of mineralizationOsteopontin is a multifaceted protein [23–25], whichhas been associated ... mamma-lian osteopontin and fish osteopontin-like proteins was indicated through an in silico analysis of available sequences showing similar gene and protein structures and was further demonstrated...

Báo cáo khoa học: Identiﬁcation of substrates for transglutaminase in Physarum polycephalum, an acellular slime mold, upon cellular mechanical damage ppt

... Aeschlimann P, Langley M,Blain E, Errington R, Kipling D & Aeschlimann D(2004) Cross-linking and G -protein functions of trans-glutaminase 2 contribute differentially to fibroblastwound healing ... by CBB staining (right) and immunoblotting analysis using each polyclonal antibody (left): (A) anti-CBP40;(B) anti-Physarum actin; (C) anti-PpANT. The closed arrows indicate each protein. The ... Caenorhabiditiselegans ERp60 homolog protein disulfide isomerase-3has disulfide isomerase and transglutaminase-like cross-linking activity and is involved in the maintenance of body morphology....

Báo cáo khoa học: Identiﬁcation of an atypical insect olfactory receptor subtype highly conserved within noctuids potx

... E, Bohbot J, Francois MC, Cain AH &Nagnan-Le Meillour P (2000) Characterization of thegeneral odorant-binding protein 2 in the molecularcoding of odorants in Mamestra brassicae . Eur JBiochem ... found in beneficial insectssuch as the honeybee [17], thus precluding the use of molecules interfering with OR83b receptors.Another subfamily of insect ORs, restricted tomoths, can also be distinguished ... loop of certain types of GPCR may be critical for ligandbinding and affinity [38]. Thus, if the classical GPCRtopology of OR18 were confirmed, OR18 EC2 mightalso play an important role in ligand...

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... membrane proteins of theINM. The INM proteins form complexes, transientlyor stably, with lamins, chromatin proteins and a vari-ety of regulatory proteins, including transcriptionalregulators and ... with theactin-binding spectrin-repeat proteins nesprin 1a andnesprin 2 [28,29].Since the different binding regions overlap at leastpartially, a simultaneous binding of some binding part-ners ... emerin.Emerin is the product of a gene linked to EDMD[19,21]. An integral membrane protein speciﬁcally loca-ted at the inner nuclear membrane, emerin, like otherINM proteins, binds to lamins....

Báo cáo khoa học: Identiﬁcation of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx

... of interacting partners andthe coordination of their activities. MAGUK contain anumber of protein protein interaction domains, suchas PSD-95, disc-large, ZO-1 (PDZ), Src-homology 3and guanylate ... wereidentiﬁed as containing the C-terminal region of thehuman MEK2 cDNA. To analyze in more detail theinteracting sites within hDlg and MEK2, mutant deriv-atives of PGKBT7-PDZ-1-2 and pACT2-MEK2 ... activating domain. (C) The PDZ-1-2 domains of hDlg fused to the GAL4 DNA-bind-ing domain were co-expressed in yeasts with the C-terminus of MEK2, encompassing the PDZ binding sequence, which was intact...

Báo cáo khoa học: Identiﬁcation of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx

... lane 5, 5 lg of nonprecipitatedproteins; lane 6, 5 lg of protein from washing step 1; lane 7, 5 lg of protein from washing step 2; lane 8, half volume of the elutedproteins; lane 9, 30 lg of ... arrow indicates IB4-binding activity): lane 1, native lightmembranes; lane 2, light membranes after a 2 min incubation with proteinase K; lane 3, light membranes after a 5 min incubation with proteinase ... precipitated) proteins;lane 5, combined washing fractions; lane 6, half of all proteins elu-ted under Ca2+withdrawal; lane 7, half of all proteins eluted with 4· sample buffer; lane 8, half of all proteins...

Báo cáo khoa học: Identiﬁcation of sodium salicylate as an hsp inducer using a simple screening system for stress response modulators in mammalian cells pptx

... blot of Luc protein in the supernatant of cells treated with or without 30,45 and 60 mMSA at 37 °C.Table 1. Eﬀect of geldanamycin, curcumin, or geranylgeranylacetone onHsp105 promoter in pGL105/C3H ... showed that SA induced the activation of HSF, the transcription of hsp genes and the accumu-lation of Hsps in various mammalian cells and a concomi-tant increase of thermoresistance of cells. Thus, ... activity in cells, suggesting that theincrease of Luc activity by SA reﬂect the levels of transcription and translation of Hsp105, not due to an indirect effect of SA on the basal activity of Luc....

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... DNA-binding motif in nucleolin [21–27]. The RGG domain of FMRP, which is an RNA-binding protein involved in nerve cell differentiation,interacts with the G-quartet forming RNA [22–25]. In addition, ... 4 and 6) was incubated with (lanes 1, 2, 5 and 6) or without (lanes 3 and 4)PRMT3 in a potassium buffer with (lanes 3–6) or without (lanes 1 and 2) AdoMet. (B) Schematic illustration of amino ... RBD and the RGG domain of nucleolin,a DNA-binding protein contributing to the transcrip-tion of ribosomal RNA, bind to the G-quadruplexforming ribosomal DNA [26]. Moreover, nucleolinbinds...

Tài liệu Báo cáo khoa học: Identiﬁcation of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc

... Zeocin resistance cassette InvitrogenpSlpxX-zeo Zeocin resistance gene inserted into pSlpxX This studypUC4K Kanamycin resistance cassette AmershampSlpxL-kan Kanamycin resistance gene inserted ... pWW115, Shengqing Yu for advice onthe pulmonary clearance assay, R. Morell for help with DNA sequencing, Yandan Yang for help with Southern blotting, and Lina Zhu and Yili Chen forhelp in manuscript ... LpxX and LpxL revealedthat both contained membrane-spanning regionsanchoring the proteins to the inner membrane but not in the cytoplasm of the bacterium (data not shown),which is consistent with...

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