... 353–729 of G. glabra GgbAS1 b-amyrin synthase [6], was prepared byPCR using GgbAS1 as a template, Taq DNA polymerase(Takara Shuzo, Kyoto, Japan), the primers 50-GAAGCATATCCACTATGAAGATGA-30 and ... theinternal standard. Sterol content was the total content of stigmasta-7,22-dien-3b-ol, stigmasta-7,25-dien-3b-ol, and stigmasta-7,22,25-trien-3b-ol, which are D7-phytosterolscharacteristic of ... Hiraoka2, Yasumasa Ikeshiro2, Kazufumi Yazaki3,Shigeo Tanaka4, Tetsuo Kushiro5, Masaaki Shibuya5 and Yutaka Ebizuka51Gifu Pharmaceutical University, Japan;2Niigata College of...