Báo cáo khoa học: Identiﬁcation, characterization and activation mechanism of a tyrosine kinase of Bacillus anthracis docx

... of Bacillus anthracis FEBS Journal 275 (2008) 6237–6247 ª 2008 The Authors Journal compilation ª 2008 FEBS 6247 Identiﬁcation, characterization and activation mechanism of a tyrosine kinase of ... as Walker A, A and B,with some exceptions [4]. The majority of the bacterial tyrosine kinases possess a transmembrane domain and an intracellular catalytic domain [3,4]. These twodomains are ... proteins also lack tyrosine kinase activity. However, the blast search of McsB and its activator McsA in the B. anthracis databaserevealed two orthologs, BAS0080 (81% similarity) and BAS0079 (69%...

Báo cáo khoa học: Identiﬁcation, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... I-D-AEA-K R-DD-QNTIVK-VGATAASASA 122 A Active siteR101BNRI/MHvTrxh1HvTrxh2Ta a Os a OsbTdTabTacTadHv a ScLpHbOscCKN A ZmOseOsdLcPcAtaAtbAtcAtdAteAtfAtgBnaBoBnbBraBrbPmFeCmNtaPtAthAtiRcNtbNtcPpPsaPsbPscPsd*Fig. ... purifiedprotein was missing methionine and alanine from theN-terminus. This was confirmed by the N-terminalsequences of HvTrxh2, determined to be AASATAAAVA and ASATAAAVAA. Multiple peaks were observed ... foramplification of the HvTrx2 coding sequence as above. A second PCR with Pfu DNA polymerase and primers trxh1(TTCATATGGCGGCGTCGGCAACGGCG) and trxh2(GGGGATCCTGAGCGGCAATTTTATTTAGGCG)was used...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... 710–728RpCAtrRq a TCA CAA ATG TCC AGT GCC AGT T 757–778Full-length sequencing of RpCAbrRpCAbrF TAC AAG GAT GCC ATT AGC 613–630RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839RpCAbrR2 AGA GCA GCA GAC CTT ACG 706–723RpCAbrR3 ... 706–723RpCAbrR3 GTT ACT TCC GCA GCT AGG 466–483Probe ampliﬁcation for FISHRpCAbrF TAC AAG GAT GCC ATT AGC 613–630RpCAbrR1 CGT AGC AGT ATC AGC AGT 822–839RpCAtrFprobe TAC AAA GAT CCA ATC CAG C ... sequenced RpCAtr (BPNJ¼ 100 and BPMP¼ 99). Fungia scutaria (FCA -a and FCA-b) and Caenorhabditis elegans (CA1 and CA2) sequences falloutside of clade I and are more closely related to eachother...

Báo cáo khoa học: Puriﬁcation, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx

... proenzyme of phenol oxidase A 1 of Drosophila melanogaster. Proc. Natl Acad.Sci. USA 92, 7769–7773.13. Kawabata, T., Yasuhara, Y., Ochiai, M., Matsuura, S. & Ashida,M. (1995) Molecular cloning of ... bp of the 5¢untranslated regions and the 3¢ untranslated regionscontaining polyadenylation signals (AATAAA) at threepositions and the poly (A) -tails. The criteria for a consensustranslation ... limpet (a marine gastropod, Megathura crenulata)as an immunotherapeutic agent for the treatment of bladdercarcinoma [27], and other observations suggesting thatCorrespondence to T. Naraoka, Aomori...

Báo cáo khoa học: Identiﬁcation, structure and differential expression of novel pleurocidins clustered on the genome of the winter ﬂounder, Pseudopleuronectes americanus (Walbaum) ppt

... CATCGTCATGTTTGAACCRTWF5. 1a/ 3¢ PFIKPR a CCTGGGTTTAATAAATGGActin ActF(WF) AALVVD TCGCTGCCCTCGTTGTTGACActR(WF) VLLTEAP a GGAGCCTCGGTCAGCAGGA a Primer based on complement.Table 2. Properties of ... estimate the net charge K and R were assumed to have a value of + 1, H of + 1/2, D and E of )1, and C-terminal amidation was counted as an additional +1.Label Residues Amino acid sequence MrChargeWF1-like a 24 ... SFDDNP a GGGTTGTCATCGAATGAGWFX RTWFX RSTEDI CGTTCTACAGAGGACATCRTWFX/3¢ DDDDSP a GGGGCTGTCATCATCATCWFY (Y1) RTWF5.1/5¢ IVMFEP CATCGTCATGTTTGAACCRTWF5.1/3¢ GYLNAA a GGCCGCATTGAGATAACCWFZ (Y1)...

Báo cáo khoa học: Puriﬁcation, characterization and biosynthesis of parabutoxin 3, a component of Parabuthus transvaalicus venom pptx

... C-terminal arginine,was designed as follows (Fig. 3A) . Two overlappingoligonucleotide pairs 5¢-GAGGTCGACATGCGCTGCAAGTCGTCGAAGGAGTGCCTGGTCAAGTGCAAGCAG-3¢,3¢-CTCCAGCTGTACGCGACGTTCAGCAGCTTCCTCACGGACCAGTTCACGTTCGTCCGCTGCCCGGCC-5¢ ,and5 ¢-GCGACGGGCCGGCCGAACGGCAAGTGCATGAACCGGAAGTGCAAGTGCTACCCGTGAG-3¢,3¢-GGCTTGCCGTTCACGTACTTGGCCTTCACGTTCACGATGGGCACTCCTAG-5¢,respect-ively, ... 5¢-GAGGTCGACATGCGCTGCAAGTCGTCGAAGGAGTGCCTGGTCAAGTGCAAGCAG-3¢,3¢-CTCCAGCTGTACGCGACGTTCAGCAGCTTCCTCACGGACCAGTTCACGTTCGTCCGCTGCCCGGCC-5¢ ,and5 ¢-GCGACGGGCCGGCCGAACGGCAAGTGCATGAACCGGAAGTGCAAGTGCTACCCGTGAG-3¢,3¢-GGCTTGCCGTTCACGTACTTGGCCTTCACGTTCACGATGGGCACTCCTAG-5¢,respect-ively, ranging in ... vector-related fragments. In a parallelexperiment with AgTx2, chromatographic proﬁles of rAgTx2 and commercially available rAgTx2 (AlomoneLaboratories) under the same conditions were comparedand...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... CCATGTCTGCAGATGGTCGAGG 5¢-RACEZf5¢stat6-R2 GGACTGACATTGCTCCAGAGC 5¢-RACEZf3¢stat6-F3 GCTTCAGTGACTCAGAAATTGG 3¢-RACEZf3¢stat6-F4 GTCCAGAATATTCAGCCTTTCACC 3¢-RACEZftbet-F1 CTCCCTCAAACAAACCAGAGTC Initial PCRZftbet-R1 ... CGAGCAGGAGATGGGAACC Real-time PCRZfbactin-R CAACGGAAACGCTCATTGC Real-time PCRZfgapdh-F CGCTGGCATCTCCCTCAA Real-time PCRZfgapdh-R TCAGCAACACGATGGCTGTAG Real-time PCRZFil4-F CATCCAGAGTGTGAATGGGA ... CTGCTTTTCTGGGGACTTCA Initial PCRZf3¢foxp3-F1 TGAAGTCCCCAGAAAAGCAG 3¢-RACEZf3¢foxp3-F2 GTGCTTTGTGCGTGTTGAAG 3¢-RACEZf5¢foxp3-R1 TGTATGATGGAAAAGGTGGCA 5¢-RACEZf5¢foxp3-R2 GGAACACACAGAGGGGATGATA 5¢-RACEOligo...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... transcriptional co-activator P ⁄ CAF potenti-ates TGF-beta ⁄ Smad signaling. Nucleic Acids Res 28,4291–4298.39 Kahata K, Hayashi M, Asaka M, Hellman U,Kitagawa H, Yanagisawa J, Kato S, Imamura ... DeMarco R, Martins EA,Guimaraes PE, Ojopi EP, Paquola AC, Piazza JP,Nishiyama MY Jr, Kitajima JP, Adamson RE et al.(2003) Transcriptome analysis of the acoelomate humanparasite Schistosoma ... day and 35 day para-sites, adult worm pairs, separated adult female and male worms, and eggs. cDNA from uninfected B. glabrata snails served as a neg-ative control.J. M. Carlo et al. SmSmad1B,...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide ... bp wasproduced by PCR ampliﬁcation with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), puriﬁed from agarose ... Extraction kit (Qiagen, Valencia, CA, USA) and randomly labeled with32P using a Metaprime kit (Amer-sham Pharmacia Biotech Inc., Piscataway, NJ, USA). ForBAC DNA sequencing, the BAC clone was...

Báo cáo khoa học: Identiﬁcation and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

... 25–29.12 Kawakami R, Sakuraba H, Kamohara S, Goda S,Kawarabayasi Y & Ohshima T (2004) Oxidative stressresponse in an anaerobic hyperthermophilic archaeon:presence of a functional peroxiredoxin ... the ﬁrst of all Prxs analysedso far in archaea that has only one cysteine residue inthe sequence.Transcriptional analysis of bcp2 under oxidativestress and characterization of mRNA 5¢ endIn ... Garrett RA (2005) Diver-gent transcriptional and translational signals inArchaea. Environ Microbiol 7, 47–54.17 Bell SD (2005) Archaeal transcriptional regulation – vari-ation on a bacterial theme?...

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