Báo cáo khoa học: Characterization of inhibitory mechanism and antifungal activity between group-1 and group-2 phytocystatins from taro (Colocasia esculenta) pdf

... Journal compilation ª 2008 FEBS 4989 Characterization of inhibitory mechanism and antifungal activity between group-1 and group-2 phytocystatins from taro (Colocasia esculenta) Ke-Ming Wang1, Senthil ... peptide (group-2) (Fig. 2B).Although antifungal activity of phytocystatins from taro, strawberry and chestnut has been reported previ-ously [7,9,17], the mechanism of inhibitory activity of phytocystatins ... and Nt (group-1) peptides on papain activity, an inhibitory mechanism of group-2 phytocystatins and a regulatory mechanism of extended Ct pep-tide have each been proposed. By contrast, the antifungal...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade.AcknowledgementsThanks are due to Prof G.L. Sottocasa and Dr Anto-nella Bandiera (University of Trieste) ... – University of Trieste) for the immunization and bleeding of rabbits; to Silvia Zezlina for the afﬁn-ity puriﬁcation of antibody A from rabbit sera; to DrPaolo Ermacora and Prof Giorgio Honsell ... ÀðkA=k0Þ=½Að1Þwhere kA and k0are the inactivation rate constantseither in the presence or in the absence of variousconcentrations of a ligand A, k2 and k1are therate constants of the inhibition of the...

Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot

... r an ging from 5.9 to 6 .1 (Fig. 3).Fractions e and f have at leas t one unique isoform e ach ( pI5.9 for e and pI 6 .1 for f), while two bands of pI 5.95 and pI6.0werecommontoeachofthemajorCLAPfractions,although ... otemperatures and pH normally inconsistent with CL activity. Primary sequence analysis of CLAP and cDNAclones coding for CLAP s how it to be a cell adhesionprotein and member of the fasciclin I family of ... standard. Cysteine protease activity of column fractions was determined using protaminesulfate as substrate and the trinitrobenzene sulfonic acid(TNBS) method [23]. One unit of protease activity...

Báo cáo khoa học: Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identiﬁcation of kinase substrates doc

... level activity (100 %) and compared with the activity of mutated enzyme StkPK42R(lane 2) and StkP in the presence 0.1 mM,1mM and 10 mM of staurosporine (lanes 3, 4, and 5, respectively), a ... of RNAP and S1 protein has already been described inE. coli and resulted in signiﬁcant stimulation of theRNAP transcriptional activity from a number of pro-moters in vitro [24].Table 1. List of ... Fig. 3. Transcriptional and functional coupling of StkP and PhpP. (A) RT-PCR analysis of stkP and phpP expression. Total RNA was extracted from cells grown in CTM medium and harvested in precompetent...

Báo cáo khoa học: Characterization of testis-speciﬁc serine–threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling doc

... Burgering3 and Lech Trzeciak11 Department of Molecular Biology, International Institute of Molecular and Cell Biology in Warsaw, Poland2 Laboratory of Molecular Medicine, Institute of Biochemistry and ... Ser166 and Thr168 locatedwithin a T-loop play a signiﬁcant role in the regulation of TSSK3 activity and suggest a similar mechanism of activation to that of the AGC kinase family.For a number of ... control of phosphorylation. Mid-dle: bands of phosphorylated MBP by TSSK3 mutants were excised from gel and their radioactivity was measured by scintillation counting.Data are representative of...

Báo cáo khoa học: Characterization of a Cry1Ac-receptor alkaline phosphatase in susceptible and resistant Heliothis virescens larvae potx

... was a result of decreased H vALP protein l evels, we compared HvALP from YHD2, YDK, and larvae from the F1 generation of backcrosses between YDK and YHD2 adults, usingimmunodetection and alkaline ... membrane-bound form of alkaline phosphatase from B. mori recognized HvALP in BBMV from YDK, YHD2 and F1larvae. No d ifferences in intensity of recognitionwere observed between HvALP from YDK and F1vesicles,while ... Comparison of HvALP levels and alkaline phosphatase activity between BBMV from susceptible and resistant H. virescens larvae.BBMV proteins from YDK (lane 1), YHD2 (lane 2), F1generation of YDK...

Báo cáo khoa học: Characterization of sequence variations in human histone H1.2 and H1.4 subtypes pptx

... and 0.1%(v ⁄ v) trifluoroacetic acid). The concentration of solvent B wasincreased linearly from 15 to 23% during a time-period of 25 min, from 23 to 70% during a time-period of 45 min and from ... and 0.1% (v ⁄ v) trifluoroaceticacid]. The concentration of solvent B was increased linearly from 15 to 23% during a time-period of 25 min, from 23 to70% during a time-period of 45 min and from ... acetonitrile and 0.1% (v ⁄ v) trifluoroacetic acid]. The concentra-tion of solvent B was increased linearly from 5 to 20% during atime-period of 65 min and from 20 to 100% during a time-period of 25...

Báo cáo Y học: Characterization of four substrates emphasizes kinetic similarity between insect and human C-domain angiotensin-converting enzyme pptx

... CarmonaKaraoglanovic2, Liliane Schoofs1, Arnold De Loof1 and Roger Huybrechts11Zoological Institute of the Catholic University of Leuven, Laboratory of Developmental Physiology and Molecular Biology, ... (yp3) of the bluebottle fly Calliphora vicina,yp3 and yp2 of the housefly Musca domestica and yp1 of Table 1. Purification procedure of several ACE-competitive peptides. HPLC purification of several ... yp3 of Musca domestica andyp1andyp 2of several Drosophila species. Finally, LEQIYHL was mostsimilar to Drosophila yp1.A multiple alignment of the purified peptides with yp1, yp2 and yp3 of Musca...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... PC and increased GPC, indicating an imbal-ance between PL synthesis and degradation and, con-sequently, an increase of TG. Other studies suggestthat the simultaneous accumulation of ML and ... extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D1H NMR spectra of extracted lipids (B) and PCA extracts (B¢) from one representative sample of MCF-7 cells. ... 1.22 and 1.40 p.p.m. (Table 1 and Figs 2 and 3). These signals most likely arise from the aggregation of large molecules because they are characterized bylarge linewidths (Table 1 and Figs 2 and...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... origin and a high con-servation of this class of proteins at both the structural and functional levels. Given the multiplicity of CLPsin humans and insects [14], we hypothesized thathomologues of ... sequence of about 90 amino acids of unknown function. The high degree of identity of theCg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden-tity) argues for a conservation of the tertiary structure and ... gigas.In this article, we report the characterization of anew CLP, named Cg-Clp2, from the oyster C. gigas.The tissue distribution and temporal pattern of expres-sion of the gene encoding Cg-Clp2...

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