Báo cáo khoa học: Mouse RS21-C6 is a mammalian 2¢-deoxycytidine 5¢-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine pdf

... methylation. Proc Natl Acad SciUSA 95, 8727–8732.37 Matsumoto M, Hatakeyama S, Oyamada K, Oda Y,Nishimura T & Nakayama KI (2005) Large-scale analy-sis of the human ubiquitin-related proteome. ... 37965–37973. A mammalian dCTPase that prefers 5-iodocytosine M. Nonaka et al.1666 FEBS Journal 276 (2009) 1654–1666 ª 2009 The Authors Journal compilation ª 2009 FEBS Mouse RS21-C6 is a mammalian 2¢-deoxycytidine 5¢-triphosphate ... were measured and areshown as percentages of the highest value. Each point and errorbar indicates the mean and standard deviation of three reactions. A mammalian dCTPase that prefers 5-iodocytosine...

Tài liệu Báo cáo khoa học: Human Cdc45 is a proliferation-associated antigen pdf

... in histological material is a valuable component of conventional histopathologi-cal analysis and may be of major prognostic import-ance [56]. Proliferation in immunohistochemicalsections can ... antibody on formalin-ﬁxed andparafﬁn-embedded tissues (Fig. 9). Antibodies againstPCNA and Cdc45 stained malignant cells in a compar-able manner, e.g. on invasive-lobular mamma carci-noma sections ... ⁄ rab-bit ⁄ rat IgG) conjugated with Cy2 ⁄ Cy3, goat anti-(rat IgG)conjugated with horseradish peroxidase (HRP) (allDianova, Hamburg, Germany) and goat anti- (mouse ⁄ rab-bit IgG) conjugated...

Tài liệu Báo cáo khoa học: KIPase activity is a novel caspase-like activity associated with cell proliferation doc

... substrate for caspase 1;Ac-IETD-AMC is a substrate for caspase 8 and 10;Ac-LEHD-AMC is a substrate for caspases 2, 4, 5 and 9.Ac-DVPD-AMC, Ac-DPSD-AMC and Ac-ESQD-AMCare tetra peptide substrates representing ... follows: caspase 1, WEHD-AMC; caspase 2, VDVAD-AMC; caspase 3, DEVD-AMC; caspase 4, WEHD-AMC; caspase 5,WEHD-AMC, caspase 6, VEID-AMC; caspase 7, DEVD-AMC; ca-spase 8, IETD-AMC; caspase 9, LEHD-AMC; ... human caspasestested (1–10) are able to cleave a speciﬁc KIPase substrate(Ac-DPSD-AMC) and that an aldehyde modiﬁed derivativeof the DPSD tetra peptide is unable to inhibit caspases, but is a...

Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt

... TAT ATG T Weak 3 aa2 ⁄ 4 55067 TCA ATG C Weak 58 aa3 33 39% 609296093360939GTA ATG CTGC ATG TTCC ATG GGAdequateWeakAdequate13 aa33 aa31 aa3¢ 76 49% –4¢ 56 61% 61041 GGA ATG T Adequate ... [13].PositionKozak consensus A GCC ATG GGContextATG1330 AAG ATG A AdequateATG2345 CTG ATG T WeakATG3396 CCC ATG A WeakATG4489 CTG ATG A WeakHu-K4 A. Munck et al.1722 FEBS Journal 272 (2005) ... obviousretrieval signal is missing.The human phospholipases D1 and D2 are mainlyassociated with the plasma membrane or with themembranes of intracellular organelles although theylack a transmembrane...

Báo cáo khoa học: Xenopus Rbm9 is a novel interactor of XGld2 in the cytoplasmic polyadenylation complex pptx

... RNA helicase in translational repression is anRNA-mediated oligomer. Nucleic Acids Res 32, 1325–1334.5 Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y,Nagahama Y & Yamashita M (2003) ... J & WickensM (2004) Mammalian GLD–2 homologs are poly (A) polymerases. Proc Natl Acad Sci USA 101, 4407–4412.18 Nakanishi T, Kubota H, Ishibashi N, Kumagai S,Watanabe H, Yamashita M, Kashiwabara ... England Biolabs (Ipswich, MA,USA) (9106S). The b-tubulin and HA antibodies wereobtained from E7 (Iowa Hybridoma Bank, Iowa City,IA, USA) and 12CA5 (Abcam, Cambridge, MA, USA)hybridomas, respectively....

Báo cáo khoa học: Syndecan-4 is a signaling molecule for stromal cell-derived factor-1 (SDF-1)/ CXCL12 pptx

... stromalcell-derived factor (SDF) -1alpha. Glycobiology 10, 21–29.34 Valenzuela-Fernandez A, Palanche T, Amara A, Mage-rus A, Altmeyer R, Delaunay T, Virelizier JL, BaleuxF, Galzi JL & Arenzana-Seisdedos ... byT-cell-line-adapted HIV-1. Nature 382, 833–835.7 Pablos JL, Amara A, Bouloc A, Santiago B, Caruz A, Galindo M, Delaunay T, Virelizier JL & Arenzana-Seis-dedos F (1999) Stromal-derived factor is ... glycosaminogly-cans activates a p44 ⁄ p42 mitogen-activated protein kin-ase-dependent signaling pathway and enhances humanSyndecan-4 is an auxiliary receptor for SDF-1/CXCL12 N. Charnaux et al.1950...

Báo cáo khoa học: Cytochrome b6f is a dimeric protochlorophyll a binding complex in etioplasts doc

... indicating a structural or a functional role for bothpigments. A structural role was indicated in a Chl-less mutant that was reported to lack accumulation of theCyt b6f complex in Clamydomonas ... phyty-lated chlorophyll precursor protochlorophyll a, and not chlorophyll a ,is associated with subunit b6. The data imply that a phytylated tetrapyrrol is an essential structural requirement for assembly ... performedTLC analysis of chromophore standards against anorganic extract isolated from the dimeric Cyt b6fcomplex for chromophore identiﬁcation (Fig. 4). Inparallel, the standards and pigment extracts...

Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

... Bacterial IscU is a well folded and functional single domain proteinSalvatore Adinolﬁ1, Francesca Rizzo1, Laura Masino1, Margie Nair1, Stephen R. Martin1, Annalisa Pastore1and ... here a structural characterization of thisprotein and demonstrate that E. coli IscU can be obtainedas a recombinant well-folded protein. We demonstrate that our construct can function as a scaffold ... mMNaCl and 10 mM2-mercaptoethanol). Each meas-urement was repeated after 6 h to ensure that equilibriumhad been reached and that proteolysis was not occurring. Inall datasets, the absorbance...

Báo cáo khoa học: Intermittent hypoxia is a key regulator of cancer cell and endothelial cell interplay in tumours pot

... the last few years, a newconcept has arisen, showing ﬁrst that changes in po2level are not always sustained in tumours but that theycan be transient, and second that intermittent hypoxiacan ... radiation.Cancer Res 59, 3374–3378.101 Kanaan A, Farahani R, Douglas RM, Lamanna JC &Haddad GG (2006) Effect of chronic continuous orintermittent hypoxia and reoxygenation on cerebralcapillary ... extracellular matrix degra-dation (matrix metalloproteases) [75–78]. In addition,NF-jB activation was reported as an early event inmalignant transformation in vitro [79], and continuousactivation...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... consideration for important pro-teomics research.AcknowledgementsWe thank Barbara Baird, Ian Jardine, Neil Kelleher,Harold Scheraga and Klaas van Wyck for valuablediscussions, and the General ... (229 kDa).AbbreviationsBCA, bovine carbonic anhydrase; CAD, collisionally-activated dissociation; ECD, electron-capture dissociation; HAD, 3-hydroxyanthranilate-3,4-dioxygenase; IRMPD, infrared ... on a peptide containing these amino acids,these data would have been ignored in most protocols.However, even measuring a molecular mass value that is the same as that predicted is not a guaranteethat...

Xem thêm