Báo cáo khoa học: The crystal structure of a xyloglucan-speciﬁc endo-b-1,4glucanase from Geotrichum sp M128 xyloglucanase reveals a key amino acid residue for substrate speciﬁcity potx

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... picolinic acid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, i.e. transformation ... by the action of ACMS decarboxylase (ACMSD, also known as picoli-nate carboxylase; EC 4.1.1.45) into a- aminomuconicKeywordscerebral malaria; kynurenine pathway;metal-dependent amidohydrolase; ... only a key precursor of NAD, but also a potent neurotoxin that acts by acti-vating the N-methyl-d-aspartate subtype receptor for glutamate [4]. QA imbalance was reported to beassociated with a...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCATGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTTACGCAGCTTATGC-3¢ for the c subunit. The segmentswere digested with NdeIandBglII ... Poznanskaya, A. A., Yakusheva, M.I. & Yakovlev, V .A. (1977)Study of the mechanism of action of adenosylcobalamin-depen-dent glycerol dehydratase from Aerobacter aerogenes. II. The inactivation ... suggesting again that the bound cobalamin is actuallycob(II)alamin. Substrate- and K+-binding sites Substrate propane-1,2-diol and the essential cofactor K+are bound inside the TIM barrel of the a...

Báo cáo khoa học: The crystal structure of NlpI A prokaryotic tetratricopeptide repeat protein with a globular fold potx

... 5¢-aataatccatggggagtaatacttcctggcgtaaaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢.3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgggggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttccttatccgatttttcgaagtgc-3¢. ... responsible for the sharp curvature of the array andreduced superhelical pitch, leading to the formation of a globular structure. Helix a Residues Deviationb,c A ˚AB pair W°cAB W°cAA¢Rotationd(AB) (A B¢)°Helix ... repeats are the most common number seen. The AB and AA¢Wpacking angles are responsible for curvature andsuperhelicity of the motif. (B) Amino acid sequence of NlpI from the translation of the nlpI...

Báo cáo khoa học: The crystal structure of annexin Gh1 from Gossypium hirsutum reveals an unusual S3 cluster Implications for cellulose synthase complex formation and oxidative stress response potx

... [1–4].Mammalian annexins A1 [5], A5 , and A 6 [6], as well asplant annexins from Medicago sativa [7] and Arabidop-sis thaliana [8,9], have been implicated in oxidative stressresponse. In particular, ... annexin Anx24(Ca32), but significant differencesare seen when compared to the structure of nonplantannexins. A comparison with the structure of the mamma-lian annexin AnxA5 indicates that canonical ... relatives.Calcium binding has been identified as a landmarkfeature of animal, plant, and metazoan annexin proteins. Asstructurally established for annexin A5 [16], the canonicaltype II calcium...

Tài liệu Báo cáo khoa học: The solution structure of reduced dimeric copper zinc superoxide dismutase doc

... on relaxationmatrix calculations, was used to back calculate the NOESYcross-peaks from the calculated structure to assess the quality of the structure. The ﬁnal family was made up of 30 structures ... and compared with that of oxidized human SOD. All the metal ligands are well deﬁnedin a single conformation. For all the ligands, the rmsd valuecalculated for all heavy atoms is smaller than ... dihedral angles provided a f amily of 30conformers with an rmsd from the average of 0.78 ± 0.11and 1.15 ± 0.09 A ˚ for the backbone and heavy atoms,respectively. When the rmsd is calculated for...

Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt

... Tsuruga and Shikama [21] conﬁrmedthat the fast phase of oxidation was due to the a chains and the slow phase was due to the bchains. Tsuruga et al. foundthat the beta chain of the tetramer does ... auto-oxidation of Hb A 0has been the subject of several studies. Mansouri and Winterhalter [5]reported that the oxidation of the a chains of Hb A 0was 10times faster than that of the beta chains and ... to an increase in the rate of the initial fast phase, i.e. oxidation of the a chains. The rates of oxidation are reduced in the presence of chelators of heavy metal cations in most cases. An...

Tài liệu Báo cáo khoa học: The pro-form of BMP-2 interferes with BMP-2 signalling by competing with BMP-2 for IA receptor binding pptx

... phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties of the growth factorby modulating the ability of the mature part to interact with the ... [16]. In the case of BMP-9, the pro-pep-tide appears not to alter signiﬁcantly the activity of the mature part [17]. For the pro-peptide of BMP-7, a tar-geting role to the extracellular matrix ... was assessed at a single timepoint (30 days), and neither the pharmacokinetics of proBMP-2 nor the kinetics of bone formation were anal-ysed. Thus, the fate of administered proBMP-2 in the animal...

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

... Edmonton, AB, CanadaType IIa bacteriocins, which are isolated from lactic acid bacteria that are useful for food preservation, are potentantimicrobial peptides with considerable potential astherapeutic ... speciﬁcity similar to the mature type IIa bacteriocin. The leader peptide, which consists of the ﬁrst 18 amino acids of PreCbnB2(1–64), forms a 10 -amino acid a- helix, from Val15 to Gln5, as we had previously ... Theseinvestigations were supported by the Natural Sciences and EngineeringResearch Council of Canada, the Alberta Heritage Foundation for Medical Research, CanBiocin Ltd (Edmonton, AB), and the CanadaResearch...

Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

... mixture of species atboth concentrations. The average Mrwas calculated bylinear regression using ultrascan data analysis softwareVersion 5 (Beckman Coulter), and an (assumed) partialspecific ... replication of the leading strand and synthesis of the Okazaki fragments is initiated by RNA primersmade by the specialized RNA polymerase, primase. The first primase to be identified and characterizedwas ... stearothermophilus[10] and for the monomeric DnaG-C structure repor-ted here. These data and the uniformity of the relaxa-tion rates along the amino acid sequence (Fig. 1)supported the notion of DnaG-C being a...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... iMDsoft,Sassenheim, The Netherlands) and searched the hospitalinformation system for the following: orders for sputum cul-tures or performance of a bronchoalveolar lavage for culture,or start ... clinical decisionmaking in the ICU. In that study, a questionnaire was com-pleted for each radiograph, addressing the indication for the radiograph and whether it changed the patient's management. Of ... in the design and statistical analysis of the study. MS conceived and coordinated the study and wasinvolved in the interpretation of the data and manuscript revi-sion. All authors read and approved...

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