... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and
5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers
for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT
ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT
ACTT-3¢ (antisense).
Caspase 3 activity
Cells ... intensity was determined using imagemaster
2d elite software 4.01 (Amersham Bioscience, Uppsala,
Sweden).
Statistical analysis
Data in bar graphs are expressed as...
... residues, vari-
able oxidation of methionine and variable deamidation of
asparagine and glutamine. Parent and fragment mass toler-
ances were set to 1 Da. Up to two missed cleavages and
half tryptic ... may
speculate that hmeprin has activity similar to BMP-1 ⁄
TLD-like metalloendopeptidases in that it acts as a
procollagen C protease as well as an activator of lysyl
oxida...
... light adaptation of the eye. Light adaptation also
induced the decrease of all-trans isomer both in the distal
and proximal portions of the retina. In contrast, the increase
of 11-cis ligand was ... using an ATTO AE6905C
Image Saver, and quantified with
NIH IMAGE
program. The
gel was then stained with Coomassie Brilliant Blue, and
the protein content was measured vi...
... Both end-plate potentials of the ion
trap were set at 1.5 V and the duration of the electron
pulse was 100 ms.
Data acquisition and handling
Primary data analysis was performed on a workstation
running ... ethanol, sonicated
and extracted overnight at +4 °C [23]. After centri-
fugation, the pellet was discarded and a sample of the
supernatant was withdrawn for radi...
... TSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYAN-PALKVNPGHSSEKAAPGT 335
E.
coli 277 TSIPGVFAAGDVMDHI YRQAITSAGTGCMAALDAERYLD GLADAK 321
A.
pernix 283 TSIPGIFAAGDCTSMWPGFRQVVTAAAMGAVAAYSAYTYLQEKGLYKPKPLTGLK ... of the FNR (Fig. 5A) . The
Fdx ⁄ CYP17 5A1 ratio was saturated at 8 : 1, and the
turnover rate at an Fdx ⁄ CYP17 5A1 ratio of 8 : 1 was
4.9-fold greater than that at a rat...
... 8,
459–467.
10 Kanda A, Iwakoshi-Ukena E, Takuwa-Kuroda K &
Minakata H (2003) Isolation and characterization of
novel tachykinins from the posterior salivary gland of
the common octopus Octopus vulgaris. ... in the common octopus (Octopus vulgaris).
Biochem J 387, 85–91.
25 Kanda A, Takahashi T, Satake H & Minakata H (2006)
Molecular and functional characterization...
... steady-state kinetics of 6-CySeCD
catalysis and investigated the antioxidant ability of
6-CySeCD using a mitochondria injury system.
Results and Discussion
Synthesis and characterization of 6-CySeCD
The ... sulfate ⁄ ascorbate-induced mito-
chondrial damage and the swelling is decreased by
addition of 6-CySeCD.
The absorbance at 520 nm for the control group
was bas...
... & Brade, H. (1994) Preparation
and structural analysis of oligosaccharide monophosphates
obtained from the lipopolysaccharide of recombinant strains of
Salmonella minnesota and Escherichia ... OX1 is a Gram-negative bacterium
isolated from the activated sludge of a wastewater treatment
plant, and endowed with unusual metabolic capabilities for
the degradation of...
... [6,8,27] or to
any other sequences available in FASTA and BLAST
database programs at the DNA Data Bank of Jap an.
Recently, we reported the cloning and s equencing of the
gene encoding 4-amino-3-hydroxybenzoate ... release of ammonia, are a key enzyme in
the metabolic pathways of 2-amino phenol and its deriva-
tives. However, little is known about the metabolic steps...