... a linearized plasmid containing a 553-bpfragment of b3Gal-T5 cDNA, that includes the initial360 bp of the coding sequence and 192 bp of the 5¢untranslated region of the gene, placed in the ... obtained by Bio-Gel P-4 purification of the included volume of the column in (A), largeO-glycans (j), obtained as the included volume of the column in (C),and small O-glycans (s), obtained as the ... O-glycans, recovered in the includedvolume of the Bio-Gel P4 column, and large O-glycans,collected with the excluded volume of the Bio-Gel P4 and the included volume of the Sephadex G-50 column(Fig....