Báo cáo khoa học: Cell death-inducing DFF45-like effector, a lipid droplet-associated protein, might be involved in the differentiation of human adipocytes pdf
... levels
during the differentiation of fetal adipose tissues and
with the maturation of human primary adipocytes,
suggesting that CIDEC might be a target of PPARc
transactivation. These data suggest that ... Authors Journal compilation ª 2010 FEBS 4183
Cell death-inducing DFF45-like effector, a lipid
droplet-associated protein, might be involved in...
... sec-
tions incubated with preadsorbed primary antiserum
(Fig. 6B). The brown 3,3¢-diaminobenzidine (DAB)
staining was granular and confined to the cytoplasm
of the cells. Based on their size and shape the ... epithelial cells and
vascular endothelial cells. These data suggest an important role for Cyp4x1
in the brain.
Abbreviations
DAB, 3,3¢-diaminobenzidine; TCDD, 2,3,7,8 tetrac...
... 1 Plasmid constructs are derivatives of pDA3480 [9,10]. The 5¢ end of the transcript is 5¢-AAUUGUGAGCGGAUAACAAUUUCA-
CCAGGUAAUAAAUU
AAAUAAAAUUUAAAUAUG-3¢ for the gene variants that lack a functional ... expression in
the cases of the arginine codons CGG and AGG, such
effect is not observed for the other analysed arginine
codons AGA, CGC, CGA and CGU. The amino acid
seq...
... interactor.
Nucleolin was first described as a major nuclear pro-
tein consisting of a negatively charged N-terminal
domain, an RNA-binding domain and a C-terminal
domain rich in RGG motifs [54]. Nucleolin has been
reported ... sig-
nal. The low yields of the AP probe and of nucleolin,
together with the inevitable partial degradation of
nucleolin, mean that calculations...
... Yokogawa
T, Okuni T, Nakayama H, Takio K, Yabuki T, Kigawa
T, Kodama K, Yokogawa T, Nishikawa K &
Yokoyama S (2002) An unnatural base pair for incor-
porating amino acid analogs into proteins. ... incorporation of unnatural amino acids, have
taken advantage of advances in cell- free translation
systems. The use of tRNA, mischarged with an un-
natural amino acid through a chem...
... CCTTTGAGCACATTTCGGCAA-3¢
P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 289
5¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢
b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 665
5¢-3222 AGCCATGCCAAATGTCTCAT-3¢
Fig. 1. Dose-related ... CT, USA).
DNA quanti®cation
The DNA content of the cell layer at the end of incubation
was quanti®ed by the method of West et al .[31].After
solubilization of the...
... 1743–1752.
36 Harada H, Tamaoka A, Ishii K, Shoji S, Kametaka S,
Kametani F, Saito Y & Murayama S (2006) Beta-site
APP cleaving enzyme 1 (BACE1) is increased in
remaining neurons in Alzheimer’s disease ... diagram of the alternative processing pathways of APP. The transmembrane
APP undergoes two alternative and competing pathways of metabolism. The major and non-amyloidog...
... are
translated by a complex mixture that contains ribo-
somes and a full complement of initiation, elongation
and termination factors, as well as a full set of amino-
acyl-tRNA synthetases and other required ... other applications. In the E. coli
system programmed with plasmid DNA, the cell
extract contains or is supplemented with an RNA po-
lymerase to transcribe the gene...
... protein was
subcellularly located mainly in the Golgi apparatus and at the plasma
membrane. Pulse–chase metabolic cell labelling studies revealed that GFP–
PrP
C
was initially synthesized as a 45-kDa ... 402–409.
57 Beggah A, Mathews P, Beguin P & Geering K (1996)
Degradation and endoplasmic reticulum retention of
unassembled alpha- and beta-subunits of Na,K–ATPase
correlat...