Báo cáo khoa học: Control of transferrin expression by b-amyloid through the CP2 transcription factor pdf

... and 3). The presence of CP2 in the protein–DNA complexwas veriﬁed by adding CP2 antibody. Addition of the CP2 antibody supershifted a portion of the CP2 com-plex (lane 6), but use of IgG as ... that CP2 isable to activate the transcription of transferrin. The transferrin proximal promoter contains a CP2- response elementTo determine the responding element for the CP2- med-iated expression ... 2Oct-1p53 CP2 AP-4p300 CP2 CP2p53 CP2 AP-2+1Fig. 1. Highly conserved regions of the transferrin promoter containing putative CP2 binding motifs. (A) The highly conserved region of the human transferrin...

Báo cáo khoa học: Role of peroxidase inhibition by insulin in the bovine thyroid cell proliferation mechanism pdf

... thisassumption. On the other hand, the absence of the inhibitorin free follicle cultures, may be due to the lack of cellularproliferation, absent in this type of culture. These factssupport the hypothesis ... K, on the 105000·gsupernatant from the thyroid monolayer cell culture. None of these proteolytic enzymes affected the inhibitory power.These results were confirmed by the HPLC analysis of the semipurified ... demonstrating the absence of inhibitory effect on TPO in the protein fractions (data notshown). The absence of lipids in the structure of the peroxidaseinhibitor, discernible throughout the purification...

Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx

... some transcription factors, either at the level of their activa-tion (mainly by their ability to bind DNA) or at the level of their expression (mRNA or protein). In the lat-ter case, the speciﬁc ... presence of aminoacid(s) resulted in inhibition of the DNA binding of the involved transcription factors. By contrast, the presence of amino acid may also result in stimulation of the DNA binding of ... Families of transcription factors modulated by glutamine toregulate physiological processes. Comparison with the effects of other amino acids. The families of transcription factors modulated by glutamine...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis-cussed on the basis of the distribution of flux control for PFK, PK and LDH.ResultsPFK has no control ... and the pyruvate bran-ches. The sum of control coefficients for the three enzymes individuallywas comparable with the control coefficient found for the entire operon; the strong positive control ... no control over these fluxes at increased expression. Decreased expression of the entirelas operon resulted in a strong decrease in the growth rate and glycolyticflux; at 53% expression of the...

Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx

... intermediate by the unique action of the serpin,whereby the RCL of the serpin inserts into the majorA b -sheet causing the protease to be rapidly translo-cated from the top to the bottom of the inhibitor ... polymers of serpins, where the insertion of another molecule’s RCL takes place [8]. Both of theseresult in the irreversible inactivation (generally) of the serpins, and, in the case of the anticoagulant ... [6].In the process, the structure of the protease appears tobe deformed by being ‘crushed’ against the bottom of the inhibitor [7]. In particular, this deformation of the protease affects the geometry...

Báo cáo khoa học: Control of transforming growth factor b signal transduction by small GTPases pot

... homology 1 domain of all Smads binds to importin-b in the case of Smad3and importin-a in the case of Smad4, while mutation of the NLS blocks the ability of these proteins to enter the nucleus [95–98]. ... established through the nuclear pore with high Ran–GDP concentrations in the cytoplasmic phase of the pore which graduallydecrease along the pore [101]. In the nuclear phase of the pore, the Ran-speciﬁc ... [8–10]. The R-Smads, in turn, oligomerize with the commonpartner Smad4 and rapidly translocate to the nucleuswhere they bind to the promoters of a large variety of target genes and regulate their expression...

Báo cáo khoa học: Control of nuclear receptor function by local chromatin structure doc

... achieved. The next step isto prove their functionality and to characterize them by the presence of specific chromatin signatures. The results of the first attempts have been published during the last ... diverse.This allows the conservation of loci to serve as a goodpredictor of occupancy by the receptor in vivo [47].Furthermore, the variety of GRE sequences providesanother level of selective regulation. ... to the receptortakes place either in the cytoplasm or in the nucleusand is followed by ligand-specific changes in receptorconformation. These changes are accompanied by dis-sociation of the...

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... referencelevel for these parental enzymes. On the other hand,compared to LAC3, as the steady-state kinetic parame-ters for all the enzymes are of the same order of mag-nitude, the ratio of the total ... preserved the integrity of domain D1and the very end part of domain D3, two regions thatinteract precisely in the natural laccase fold (Fig. 5).Because of the bias introduced by linearization of the receptor ... coloured in light blue; the surface of the parts of hybrids originating from eitherLAC1, LAC2 or LAC5 but identical to LAC3 is coloured in dark blue; the surfaces of the parts of hybrids corresponding...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... affect sterol biosynthesis dur-ing the formation of a mating projection. Nevertheless, the phenotypes of mutants defective in ergosterolsynthesis clearly demonstrate the importance of sterolsfor ... beensuggested that the degree of sterol biosynthesis mayincrease in response to pheromone [28]. To test thishypothesis, we analyzed sterols from cells grown in the presence of a -factor and the solvent ... cleave off the reporter RUra3,which is fused to the PAK. Released RUra3, a modiﬁed version of the enzyme Ura3 with an arginine at the extreme N-terminus, istargeted for degradation by the enzymes...

Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... cysteine and the P-loop in the structures of PhyAsr.Table S3. Least squares superposition of main chainatoms of the P-loop (HCX5RS ⁄ T) of PTP structuresdetermined in the absence of an active ... S4). The average B-factors of the P-loop residues in the unoxidized and oxidized structures were 14.3 A˚2and15.4 A˚2, respectively. This is 4–5 A˚2lower than the overall B-factors of the ... contact with the carbonyl oxygen of Gly255.These movements away from the oxidized cysteineincrease the space inside the P-loop to accommodate the large sulfonic acid group. The movements in the P-loop...

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