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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx

Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx

... domain of t-bet, the STAT protein inter-action domain, STAT protein all-alpha domain, STATprotein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipperdomain and ... proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which arethought to help mediate DNA binding and may beinvolved in the induction of dimerization [43]. Each of these ... expression of T-bet has beenseen within a wide variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... genes from other species. For example, the loca-tion of the intron within the MH1-encoding region isconserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1Bis ... representing the 5¢- and 3¢-UTRs areshown in white in the genomic gene and the cDNA. Intron size in bp, domain size in bp and domain size in amino acids are indicated at the bottom of each schematic ... amplification of the SmSmad1BcDNA flanking the linker region did not produce mul-tiple PCR products (data not shown), indicating the absence of alternative splicing in this region. The beginning of exon...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... released from infec-Fig. 11. DNA binding of SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro. DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 ... (containing 20 amino acids at the 5¢ end of DBD, DBD and 40 amino acids at the 3¢ end of DBD), SmRXR1 and SmRXR1(Glu251-Asn376) (con-taining 20 amino acids at the 5¢ end of DBD, DBD and 40 amino ... only in RARs (NR1B) [17] (Fig. 4C). The splice site of ADNA binding domain B Ligand binding domain Fig. 1. Sequence alignment. (A) Alignment of DNA binding domain (C domain) and itsC-terminal...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

... clarified in detail and could playa key role in the detoxification processes. In this study we examined the role of Bcp2 in orderto increase the knowledge of the enzymatic activityinvolved in the ... examined the involvement of the peroxiredoxin Bcp2 in oxidative stress in the hyper-thermophilic aerobic archaeon Sulfolobus solfataricus.Furthermore, we report the cloning, the expression and ... expression and the characterization of the recombinant proteinrBcp2 in order to shed light on its role in the detoxifi-cation process and on its catalytic mechanism. Results Identification of the bcp2...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

... M.,Fujisawa,H.,Kleihues,P.&Walter,G.(2001)Reducedexpres-sion of the Aa subunit of protein phosphatase 2A in humangliomas in the absence of mutations in the Aa and Ab subunitgenes. Int.J.Cancer93, 798–804.31. Calin, G.A., De Lasio, M.G., Caprini, ... kDa instead of the calculated 72 964 Da, we verified the correctness of the expected initiation methionine by translating the fusionprotein GST-XN73 and His6-XN73. As can be seen in Fig. 2B, these ... fusion proteins run at the expected height,proving that no internal methionine was used as initiationstart in the translation of the wild type mRNA of XN73 and that the XN73 protein runs with...
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Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx

Báo cáo khoa học: Identification and characterization of plasma kallikrein–kinin system inhibitors from salivary glands of the blood-sucking insect Triatoma infestans pptx

... thattriafestin-1 and triafestin-2 inhibit the activation of the kallikrein–kinin sys-tem by inhibiting the reciprocal activation of factor XII and prekallikrein, and subsequent release of bradykinin. Binding ... of bradykinin during blood-feeding by a mechanism sim-ilar to that of hamadarin. In this study, we report two potent inhibitors of the plasma kallikrein–kinin system in salivary glands of the kissing ... pathway.Triafestin inhibits FXII and PK reciprocalactivation and bradykinin generationTo investigate inhibition of the kallikrein–kinin systemby triafestin-1 and triafestin-2, the effects of triafestin-1and...
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Báo cáo khoa học: Identification and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx

Báo cáo khoa học: Identification and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx

... different.Therefore, we investigated the relative binding proper-ties of these two types of HSA. We investigated the binding of L(small)-Trp as an endogenous ligandwhich binds to site II, and of cefazolin ... synthesis and release at the brain, and that might contribute to the pathogenesis of cisp-latin-induced emesis. These findings indicate that the state of Cys34 of HSA affects the l-Trp binding affinityon ... of cefazolin (site I-ligand) and verapamil (site I and II-ligand) as exogenous lig-ands. All these ligands are known for their high bind-ing efficiencies to HSA. The binding affinity of each compound...
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Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

Báo cáo khoa học: Identification and characterization of four novel peptide motifs that recognize distinct regions of the transcription factor CP2 doc

... 2005 FEBSRecognition of proteins containing CP2-bindingmotifs and verification of the CP2-binding ability of REST and YY1 that contain the HXPR motifAs stated above, none of the 12-mer peptide ... well charac-terized. Of the proteins that have CP2-binding motifs, wefurther investigated the CP2 binding of the twoHXPR-motif-containing proteins, REST and YYI.Both REST and YY1 function as ... backgroundGST binding from that of GST–CP2 binding. The results are presented as mean ± standard error. (C) CP2-binding activity is proportionate to the number of phage. The extent of binding to GST–CP2...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf

Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf

... recognized the 47 kDa band in T-cells, and the 40- and 47 kDa protein bands in Jurkat cells. (C) Lin-ear depiction of Ca1, Cb1 and Cb2 amino acid sequence and the localization of the domains ⁄ antigens ... thatcould be inhibited by protein kinase inhibitor (PKI). In the presence of cAMP approximately one-third of the PKI-inhibited activity was released into the extract, indi-cating that the precipitated ... antibody-immobilized Cb2 isoform wouldremain in the pellet in the absence of 8-CPT-cAMPwhile they would be released to the supernatant in the presence of 8-CPT-cAMP. The precipitates and supern-atants...
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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx

Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx

... 2779–2790 ª 2010 The Authors Journal compilation ª 2010 FEBS Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis Louise ... catalytic residues, indicating a role in binding the nucleotideinto the active site [15,16]. The crystal structure of CNS from Neisseria meningitidis crystallized in the presence of the substrate ... stereo-view of the residues of interest (blue) in the active site of the CNS from Neisseria meningitidis containing CDP(yellow) (PDB 1EYR) [12] superimposed with their equivalent residues (green) of the...
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