Báo cáo khoa học: Crystal structure of RNase A tandem enzyme

Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx

... dimeric RNases. Abbreviations BS -RNase, bovine seminal RNase; ds -RNase A, domain-swapped RNase A; FAM-AUAA-TAMRA, 6-carboxyﬂuorescein-dArU(dA) 2 -6- carboxytetramethylrhodamine; RATE, RNase A tandem ... monomeric RNase A. On the other hand, the decreased activity of the RATEs as compared with RNase A ([16] and Table 1) indicate a negative inﬂuence of the...

Ngày tải lên : 22/03/2014, 16:21

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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... atoms) and r j is the standard deviation of B factors. The normalized B factors have a zero mean and unit variance. All atoms that satisfy B z ‡ 4 are treated as outliers and discarded. After ... residue at P4, with a contact area of 13.5 A ˚ 2 and the Lys residue at P3 with a contact area of 10.2 A ˚ 2 . Residues outside the central binding area have signi...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... oligonucleotides: a sense primer 5¢-CAACAAATTTG CATATGACTATT AAAG-3¢ containing an NdeI site (underlined) next to the start codon of the T. brucei gGAPDH gene; an anti- sense primer 5¢-CAGCCAAGCG CCTAGGGAGCGAGA AC-3¢, ... Mikaelian & Sergeant [33] and using the Vent DNA polymerase. The T. brucei gGAPDH Thr196 ACA codon was changed into the Ala codon GCA, and the Thr225...

Ngày tải lên : 20/02/2014, 02:21

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Báo cáo khoa học: Identiﬁcation of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx

... microsomal rat liver carboxylesterases and their activity with retinyl palmitate Sonal P. Sanghani, Wilhelmina I. Davis, Natividad G. Dumaual, Alan Mahrenholz and William F. Bosron Department of Biochemistry ... protein with coomassie blue and the duplicate half was stained for activity as described for nondenaturing PAGE. The pI for each isoenzyme was calculated from the...

Ngày tải lên : 08/03/2014, 10:20

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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... both the size and the shape of the pocket (Fig. 5A, B). Leu297 in TaLcc1 (Ala297 in MaL) narrows the cavity as compared with MaL, whereas Pro195 and Val428 (Phe194 and Phe427 in MaL) make the cavity ... (asco-laccases) have been rather unexplored, and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We have now solv...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... trigonal bipyramidal coordination with ﬁve ligands: four side chain atoms and a water oxygen. One aspartate and two histidine side chains ligated to the central metal on the equatorial plane. In the ... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... and hydrolyze metal-free phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two domains [4,5]. ... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... and the human airway trypsin (HAT) ⁄ HAT-like ⁄ DESC subfamily. Members of the hepsin and matriptase subfamilies are known structurally and here we present the crystal structure of DESC1 as a ... regions of these proteinases medi- ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main c...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al. (2003) Genome sequence of Bacillus cereus and comparative analysis with ... hydrogen-bon- ded antiparallel b-strands (b6–b7), an a- helical hairpin (helices a5 , a6 ) and a C-terminal strand (b8). Helix a5 and strand b7 partially cover one side o...

Ngày tải lên : 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... extreme thermal motion. As a response, they have evolved enzymes that are highly stable against heat and other denaturants. The increased stability of enzymes from thermo- and hyper- thermophiles ... positions and gave a root mean square deviation of 0.84–1.21 A ˚ (Table 2, Fig. 2). The structural resemblance with regard to root mean square deviation, fraction of c...

Ngày tải lên : 19/02/2014, 16:20

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