Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot
Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot
... RNA binding and protein folding in RNA UDE.Interestingly, treatment of RNA UDE with RNa- se A did not result in significant changes of the meltingcurve, indicating that the characteristics of the ... are in excel-lent agreement with the hypothesis of the two confor-mational states presented in Fig. 4, and are also in line with the findings of the thermal unfolding and CDstudies. RNA binding ... with strongly bound RNA. On the basis of this finding, wedecided to investigate the effects of RNA binding on protein structure and stability in detail. RecombinantUDE is eluted in two distinct...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... Vg1RBP-associated proteins,such as the embryonic poly(A) binding protein, the Y-box RNA- packaging protein 2B and the oocyte-specific version of the elongation factor 1a(42Sp50). Incorporation of 42Sp50 into ... Vg1 mRNA in the context of the late vegetal RNA transport pathway. In the nucleus, Vg1 is recognized by the RNA- binding pro-teins Vg1RBP and hnRNP I [7,21]; the export of thiscomplex into the ... were indistinguishablefrom each other. Proteins specifically interacting with Vg1RBP were isolated and subjected to MS protein identification. Several of the protein species identifiedby this means...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
... change with tissuesource and age, but within a single chain there is vari-ability. The chain cap of CS is a GalNAc or GlcAresidue; a 4,6-disulfated GalNAc residue, rare in the repeat region of ... diverse tissue sources, fromtissues of differing ages and also within a singlechain. Enhanced availability of data to facilitate the characterization of these structures is therefore of value.GAGs ... 6-O-sulfation, is observedwhereas in C. elegans the chondroitin is unsulfated[13,14].Within a tissue, the sulfation profile and levels of epimerization of GlcA to IdoA change with age. Forexample, the...
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Tài liệu Báo cáo khoa học: Association of mammalian sterile twenty kinases, Mst1 and Mst2, with hSalvador via C-terminal coiled-coil domains, leads to its stabilization and phosphorylation doc
... severalproteins. The growth inhibitory proteins, Rassf1 and Nore1 can form complexes with and inhibit Mst1activity in an interaction involving their conservedC-termini [15,25]. Interestingly, ... advantages in mutant versus normal tissue[17,18]. The Salvador protein has domains that permit protein protein interaction, including a WW domain and a predicted coiled-coil motif in its C-terminus, ... that of Mst2 D437, is also shown. The structures of wild-type and mutant Sav constructsused in this study are shown indicating the location of the coiled-coil domain and the proline binding WW...
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Tài liệu Báo cáo khoa học: Association of feather colour with constitutively active melanocortin 1 receptors in chicken docx
... expressed in the central regions of the brain,including the hypothalamus where it is an importantregulator of food intake. The MC4 receptor shares 60% of the amino acids with the MC1 receptor, but has ... centrifuged. The intracel-lular cAMP produced was measured in 50 lLofthesupernatant after addition of [3H]cAMP and bovineadrenal binding protein and incubation for 2 h at 4 °C.Standards containing ... stimulatetyrosinase, the rate-limiting enzyme in the synthesis of bothclasses of melanin pigments, eumelanin and phaeomelanin.A low level of tyrosinase expression leads to increasedphaeomelanin synthesis,...
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Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt
... 455 The position of the AU rich region at the 5¢ end is not critical for Hfq binding The idea that stimulation of poly(A) synthesis is cor-related with the association of Hfq to the 3¢ end of RNA ... suggestedthat Hfq binding is independent of the location of the AU rich region in the RNA. This conclusion has been verified by comparing the affinity of Hfq for3¢rpsO RNA harboring a stretch of 18 A residuesappended ... containing a UUUUAAAAUGUsequence and an 11 nucleotide long linker containing 6Us and 3 As [36]. It is interesting that this RNA frag-ment, which is not known to interact with Hfq in the cell, is...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... evidence in support of binding of ATP to the ligand-free hGK, resulting in a change of protein conformation. The MD simulations indicatethat the binding triggers molecular motion of the flexi-ble ... associated with ATP binding; (c)obtaining insights into the active site contact residues involved in ATP binding; and (d) relating this informa-tion to steady-state enzyme kinetic data. To achievethese ... site of the hexokinase family of enzymes, positioned in the phosphate-binding loop and part of a classical ATP-binding motif (phosphate 2 site) in hexokinases and homologous proteins [22]. In the...
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Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx
... determine the area of the peaks after elutionfrom the column. These analyses showed that, within the accuracy of the area determination, there was a linear rela-tionship between peak areas and the ... cleft is tunnel-like, permitting tightinteraction with the polymeric substrate. The aglycon-side of the substrate-binding groove is extended by the aromatic surface of a putative chitin-binding ... can be determined as the degree of scission (a), i.e. the fraction of glycosidiclinkages in the chitosan that has been cleaved by the enzyme, by monitoring the increase in reducing endresonances...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... observablefluorescence band and a change in electrophoretic mobilitydue to the drug binding. This is attributable to the property of DAPI that favours folding of the random coil ATT strandinto a fast-migrating ... of the freemonomeric random coil structure of (ATT)6 in comparison with its hairpin conformer bound to DAPI [16].Binding mechanisms of DAPI and their effects on the stability of template intramolecular ... of Klenow synthesis is present in the lane containing the reaction products of AAT-template. In contrast at the 16 : 1DAPI/template molar ratio, DAPI inhibits synthesis of the 55-base product in the...
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Báo cáo khoa học: Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNASer potx
... increased (Fig. 2B).Pex21p increases tRNA binding by SerRSTo further investigate the in uence of Pex21p on tRNAbinding by SerRS, we compared the extent of tRNAbinding by isolated enzyme and ... associate with aaRSs. YeastTyrRS interacts with Knr4, a protein involved in cellwall synthesis [29], and heat shock protein 90 interacts with human glutamyl-prolyl-tRNA synthetase and medi-ates protein protein ... whether the positively charged C-terminal region of the synthe-tase, a characteristic of all eukaryotic SerRSs, is involved in protein binding. Our studies revealed that the removal of 13 amino...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘITÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ