... GTA GGTTGA TTT CAT GTC GAA TG-3¢; additional XbaI siteunderlined) and OB 3 (5¢-AAA AGA ATT CTT AGAAGT CCC AGT CAT CGT C-3¢; additional EcoRI siteunderlined).The amplified PCR fragment (Taq ... protein molecular weight stan-dards (Amersham Pharmacia, Piscataway, NJ, USA). Poly-clonal rabbit antiserum specific against the C. ammoniagenesRF2 protein served for immunostaining in a western ... was sequenced by a primer walkingapproach. For DNA analysis, dnastar software (DNAS-TAR Inc., Madison, WI, USA) and clone manager 5.0(Scientific & Educational Software, Cary, NC, USA) wereused....