... Thisregion of the LSR gene is deleted in the mutant allele. The Fig. 1. Generation of the LSR null allele. (A) Structure of the mouse LSR gene (top), the linearized LSR targeting vector (middle) and the ... partic-ularly that the liver lane was overloaded. Quantitation of the amount of LSR mRNA was thus performed after normal-ization of the radioactivity of the LSR bands to that of the 1.35 kb GAPDH ... by the intestine and synthe-sized endogenously by the liver, are secreted into the circulation as lipoproteins for their transport to tissues, where they are used mainly for membrane synthesis,steroidogenesis...