... enhances the CREB-repression activity of SIK1,indicating that the complete c ombination of phospho-domain 3A and intact domain 3B is important for the modulation of CREB-repression activities of ... underlines. The similaritybetween SIK1 and SIK2 in domain 3A, the N-terminal half of domain 3, is higher thanthat in domain 3B, the C-terminal half.(C) The intracellular distribution of chimeras in ... and mutant SIKs using 8 lLofLipofectAMINE 2000. After 36 h of incubation, cells werelysed in 0.7 mL of lysis buffer [50 m MTris/HCl (pH 8.0),300 m MNaCl, 5 m MEDTA, 5 m MEGTA, 2 m Mdithio-threitol,...