... Australia;2Department of Biochemistry, Lund University,SwedenDuring biosynthesis of chlorophyll, Mg2+ is inserted intoprotoporphyrin IX by magnesium chelatase. This enzymeconsists of three different ... performthe ATP hydrolysis. In addition, kinetic analysis had shownbinding of Mg2+ to this subunit [21]. An integrin I domain,suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding ... D207N, R289K and L111F, to bindtothe70kDaBchDproteinwithanN-terminalHis-tag.His-tagged proteins have affinity to immobilized Ni2+andusually remain bound to the column when it is washed2184 V....