... localizedfashion as revealed by mutational and X-raycrystallographic analysesMuhammad S. Rohman1, Takashi Tadokoro1, Clement Angkawidjaja1, Yumi Abe1,Hiroyoshi Matsumura2,3, Yuichi Koga1, ... extension: tailor-made genes using thepolymerase chain reaction. Biotechniques 8, 528–535.34 Kanaya S, Katsuda C, Kimura S, Nakai T, Kitakuni E,Nakamura H, Katayanagi K, Morikawa K & IkeharaM ... coun-terparts in a localized fashion by increasing the number of amino acidresidues unfavourable for protein stability.Abbreviations4·-RNase HI, So-RNase HI derivative with Asn29 fi Lys, Asp39...