. of Pt or Pt-D, aswell as by varying Pt or Pt-D concentrations at satur-ating NADP concentrations (data not shown). Thekinetic parameters of each data set were obtained fromfitting the data. University of Illinois at Urbana-Champaign, Urbana, IL, USA2 Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA3 Department of Biochemistry,. temperature of the var-ious stock solutions and the observation cell maintained at 25 °C by a recirculating water bath. The reaction was initi-ated by addition of 1.8–2.5 lg of WT or PTDH-E175A...