The a and b adapters are used as priming sites for both amplification
The a and b adapters are used as priming sites for both amplification
... B adapters are ligated to the < /b>
blunt ends using DNA ligase
•
The < /b> strands are denatured using sodium
hydroxide to release the < /b> ssDNA template
library (sstDNA).
The < /b> Adapters
•
The < /b> A < /b> and < /b> B adapters ... ssDNA bead to be loaded into a < /b>
well.
•
Enzyme beads and < /b> packing beads are
added. Enzyme beads containing sulfuras...
Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf
... extracellular signal-regulated kinase-5 (ERK5)
mitogen-activated protein (MAP) kinase [18].
GR assays indicated that human Hsp9 0a < /b> and
< /b> Hsp9 0b, as well as the < /b> native yeast Hsp90s, were all
capable ... mammalian
clients are able to be activated by both Hsp9 0a < /b> and
< /b> Hsp9 0b. Whether Hsp9 0a < /b> or Hsp9 0b is expressed in
the < /b> yeast, however,...
Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc
... and < /b> four
Cu(I) loaded a-< /b> and < /b> b- domains, no interaction was seen between the < /b> two
species. There was neither any indication for a < /b> net transfer of Cu(I)
between the < /b> two domains nor for the < /b> formation ... vivo, and < /b> are believed to be important for d
10
-metal
control. To date, structural information is available for the < /b> Zn(I...
Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... (3) and < /b> (4), the < /b> dissociation rate con-
stant, k, and < /b> the < /b> O
2
affinity, K , can be derived for both
the < /b> a < /b> and < /b> b subunits from the < /b> averaged parameters of
HbA oxygenation (Table 1, Average). The < /b> association
and < /b> ... HbA in
quite different ways. The < /b> decrease in the < /b> association
rate constant of BR for the...
Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... transferase) activity of the < /b> enzyme product and < /b> allowed
detection of a < /b> small UDP-Gal:Fuca1,2GalaGal transferase
(B transferase) activity. The < /b> presence of the < /b> mRNA and < /b> of
the < /b> A < /b> and < /b> B antigens was searched ... analysis.
Enzyme Species GenBank/EBI
A < /b> transferase Human J05175
A < /b> transferase Rat AF264018
A < /b> tr...
Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC
AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG
AGCCTTACAAGAAGGGTCCAAAGA-3¢). The < /b> PCR
product was purified, treated with T4 exonuclease to
create vector-compatible overhangs and < /b> annealed ... LlCBP3 3A,< /b> the < /b> fast
initial phase was maintained longer than in the < /b> absence
of LlCBP3 3A,< /b> indicating that LlCBP3 3A < /b> acts synergi...
Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... isoform-specific antibodies confirm that CnAa and
< /b> CnAb proteins are expressed by the < /b> appropriate recombin-
ant CnAa and < /b> CnAb baculoviruses (Fig. 1B, C).
Kinetic assays of CaNa and < /b> CaNb phosphatase activity
Kinetic ... titered by plaque assay as
described [10]. The < /b> coinfection, expression and < /b> purification
of baculovirus-expressed CaN containing the <...
Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt
... stromal processing peptidase (SPP)
has been characterized, and < /b> a < /b> preferred consensus
sequence for cleavage between a < /b> basic amino acid
(arginine or lysine) and < /b> a < /b> C-terminal alanine was
described ... Sequence alignment of pPORA and < /b> pPORB from barley and < /b> pPOR from pea. Arrowheads indicate the < /b> cleavage sites between the
< /b> transit pe...
Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... serving as a < /b> control. Thereafter, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as mean
relative luciferase activity, in arbritary units (RLU) ± SEM(n ¼ 6). (D) As a < /b> complementary ... cells
and < /b> HEK 293 cells. Thereafer, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as mean...
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