Báo cáo khoa học: "INTEGRATING SEMANTICS KNO FLEXIBLE SYNTAX BY EXPLOITING ISONORPHISM BETWEEN GRAIElATICAL AND SEMANTICAL RELATIONS" pot

Báo cáo khoa học: Design of expression vectors for RNA interference based on miRNAs and RNA splicing potx

... constructed by clo-ning the miR30 arms from the pSM2 (cut by SalI and MfeI) (provided by G Hannon) into the XhoI and EcoRIsites of pcDNA3.1 ⁄ myc-His(–)A (all sites were destroyed), and subsequently ... standard deviation (n ¼ 3).Fig. 4. Knockdown of endogenous PLD2 using pmiR155 and pSM155. HeLa cells were transfected with artiﬁcial miRNAs againstluciferase (control) and PLD2 in pmiR155 and ... transfection.PLD2 and a-tubulin were detected by a polyclonal antibody and amouse monoclonal antibody, respectively, followed by goat anti-rabbit secondary IgG conjugated to Alexa 680 and goat anti-mouseconjugated...

Báo cáo khoa học: Do bacterial genotoxins contribute to chronic inﬂammation, genomic instability and tumor progression? pot

... Cam-pylobacter sp. and Helicobacter sp, and S. enterica [4].CDT is generally a exotoxin, and the active holotox-in is a tripartite complex encoded by a single operon[5,6], formed by the CdtA, CdtB and CdtC ... inactivated by transposon mutagenesis. Thisresponse was characterized by an increase in transcrip-tion levels of pro-inﬂammatory (TNF-a, IFN-c and Cox-2, IL-6 and TGF-a) and anti-apoptotic (Bcl-2 and Bcl-XL) ... four-layeredfold of the DNase I family: a central 12-stranded b-sandwich packed between outer a-helices and loops oneach side of the sandwich [7]. The crystal structureconﬁrms previous data...

Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf

... wasconﬁrmed by DNA sequencing.ImmunoblottingCells were harvested by scraping from culture dishes in hot1· SDS sample buffer, and the lysates were separated by SDS–PAGE and analyzed by immunoblotting. ... model ofneuronal growth and differentiation. We found that expression of miR-221 and miR-222 expression were induced by nerve growth factor (NGF) stim-ulation in PC12 cells, and that this induction ... cycloheximide (B) and inhibition of protein synthesis by cycloheximide (C) were conﬁrmed by immunoblotting. a-Tubulinwas used as a loading control. DMSO, dimethylsulfoxide.NGF induces miR-221 and 222...

Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death docx

... proteins Bax and Bak, caspaseactivation and cell death [2,3]. In addition, the physi-ological platelet agonist thrombin also induces Bid,Bax and Bak translocation to the mitochondria and endogenous ... various time points (5, 10, 20,30 and 90 min and 6, 8 and 24 h) after treatment withnon-opsonized zymosan A or OPZ. The main changeswere detected after 30, 90 min and 24 h: only the resultsobtained ... pointsafter treatments (T0, 30, 90 min and 24 h).Mitochondrial membrane potential in living cellsThe mitochondrial membrane potential (MMP) of control and treated cells was studied using 5-5¢,6-6¢-tetrachloro-1,1¢,3,3¢-tetraethylbenzimidazol-carbocyanine...

Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death potx

... [7]. L(1–2); loop between sheets b-1 and b-2; L(2–3), loop between sheets b-2 and b-3. Asp57, Glu197 and Glu201 are residues involved in chitosan substrate binding at)2, )1 and +2 subsite, respectively ... 0, 0.22, 0.65, 1.6 and 2.6 m, and that of D40G was 8.5 lm. When D36A + D40G was usedinstead of D40G, the ﬁnal concentrations of the doublemutant and sodium azide were 26 lm and 2.3 m, respec-tively. ... consisting of twoa helices and a b sheet [8]. This structure is sharedwith GH24 enzymes, and is also found in GH19chitinases and GH22 or GH23 lysozymes, representedrespectively by the extensively...

Báo cáo khoa học: Re-evaluation of intramolecular long-range electron transfer between tyrosine and tryptophan in lysozymes Evidence for the participation of other residues doc

... containing the HEWL dimers and samplesof irradiated solutions were hydrolysed by trypsin and aminogroups were ethylated. Then fragments were separated by HPLC and identiﬁed by their amino acid sequences. ... proteinconcentration was varied between 0.05 and 0.5 mmolÆL)1.All reactions, monitored by changes in the UV–visibleregion, were functionally ﬁrst order, and the rate constantswere obtained by nonlinear least-squares ... times,dialyzed and lyophilized), and turkey egg white lysozyme(TEWL) were supplied by Sigma. Other lysozymes wereprepared using known procedures [23]. The purity of theprotein preparations was tested by...

Tài liệu Báo cáo khoa học: "Integrating Syntactic Priming into an Incremental Probabilistic Parser, with an Application to Psycholinguistic Modeling" ppt

... difference in performance between the Between and Within models and the Copymodel when beam search was used. We there-fore also state the performance for Between and Within models with binarization ... Corpus+ Adapt, Randomized Corpus- Adapt, Untouched Corpus- Adapt, Randomized CorpusFigure 3: Log of number of words between ruleinvocationstogram for both the non-randomized and random-ized ... non-randomized and randomized corpora.5.2 Results and DiscussionThe resulting plot for the Within model is shownin Figure 3. For both the positive and negativelyadapted rules, we ﬁnd that randomization...

Tài liệu Báo cáo khoa học: "Integrating Information Extraction and Automatic Hyperlinking" docx

... couple of standard operators. Con-catenation is expressed by consecutive items. Dis-junction, Kleene star, Kleene plus, and optionality are represented by the operators |, *, +, and ?, resp. ... a recognition pattern on the left-hand side, written as a regular expression, and an output description on the right-hand side. A named label serves as a handle to the rule. Regular expressions ... develop-ment and use of multilingual text processing components. By combining finite-state and unification-based formalisms, the grammar formalism used in SProUT offers both pro-cessing efficiency and...

Tài liệu Báo cáo khoa học: "INTEGRATING WITH WORD BOUNDARY IDENTIFICATION SENTENCE UNDERSTANDING" docx

... fragments in italics in (1), (2), and (3), ben- ten sheng, shi .fen, and he shang, will be wrongly identified as: ben rensheng, shi.fen, and heshang, respectively, by statistical approaches. ... understanding. Rather, the former is re- garded as one of the tasks an NLP system must handle within the understanding phase. This perspective allows us to devise a more systematic and natural ... difference between this knowledge and the one mentioned in footnote 2. Both are used to disambiguate the fragment shi .fen. The former is more ad hoc while ours comes in naturally as part and parcel...

Tài liệu Báo cáo khoa học: "Integrating Symbolic and Statistical Representations: The Lexicon Pragmatics Interface" pot

... issues we consider here are the integration of the statistical and symbolic components, and the division of labour between se- 1Here and below we base our frequency judgements on semi-automatic ... (Las- carides and Asher. 1993). This framework has already been successful in accounting for other phenomena on the interface between the lexicon and pragmatics, e.g Asher and Lascarides ... Elaboration(a, 3) + e3 C ea Subtype and Elaboration encapsulate clues about rhetorical structure given by knowledge of subtype relations among events and objects. Coherence Constraint on...

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